Purification and characterization of an endopeptidase from Pseudomonas fluorescens ATCC 948

Journal of Food Biochemistry

Volumn 22, Issue 1, 1998, Pages 17-35

  Gobbetti, Marco ^a   Smacchi, Emanuele ^a   Stepaniak, Leszek ^b   Crea, Francesca ^a   Fox, Patrick F ^c  

^a Institute of Dairy Microbiology   (Italy)

^b Norway Agricultural University   (Norway)

^c UNIVERSITY COLLEGE CORK   (Ireland)

Author keywords

[No Author keywords available]

Indexed keywords

PSEUDOMONAS; PSEUDOMONAS FLUORESCENS;

EID: 0032327503     PISSN: 01458884     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1745-4514.1998.tb00228.x     Document Type: Article

References (34)

1. ATLAN, D., LALOI, P. and PORTALIER, R. 1989. Isolation and characterization of aminopeptidase-deficient Lactobacillus bulgaricus mutants. Appl. Environ. Microbiol. 55, 1717-1723.
2. BAANKREIS, R. 1992. The role of lactococcal peptidases in cheese ripening. Ph.D. thesis. University of Amsterdam, the Netherlands.
3. BARRET, A.J. and RAWLINGS, N.D. 1992. Oligopeptidases, and the emergence of the prolyl oligopeptidase family. Biol. Chem. Hoppe-Seyler. 373, 353-360.
4. BRADFORD, M.M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72, 248-254.
5. CHOPRA, A.H., CHANDER, H. and SINGH, J. 1982. Lipolytic activity of Syncephalastrum racemosum. J. Dairy Sci. 65, 1890-1895.
6. FOX, P.F., SINGH, T.K. and MCSWEENEY, P.L.H. 1995. Biogenesis of flavour compounds in cheese. In Chemistry of Structure-Function Relationships in Cheese, (E.L. Malin and M.H. Tunick, eds.) pp. 59-97, Plenum Press, New York.
7. GOBBETTI, M. 1995. Purification and characterization of intracellular carboxypeptidase from Pseudomonas fluorescens ATCC 948. Ann. Microbiol. Enzymol. 45, 13-26.
8. GOBBETTI, M., CORSETTI, A. and FOX, P.F. 1995a. Purification and characterization of an intracellular aminopeptidase from Pseudomonas fluorescens ATCC 948. J. Dairy Sci. 78, 44-54.
9. GOBBETTI, M. and FOX, P.F. 1996. Purification and characterization of a dipeptidase from Psueodomonasfluorescens ATCC 948. J. Dairy Sci. 79, 357-365.
10. GOBBETTI, M., SMACCHI, E., CORSETTI, A. and BELLUCCI, M. 1997. Inhibition of proteolytic enzymes from Pseudomonas fluorescens ATCC 948 and angiotensin I-converting enzyme by peptides from zein, hordein and gluten hydrolyzates. J. Food Prot. 60(3), 499-504.
11. GOBBETTI, M., STEPANIAK, L., FOX, P.F. SØRHAUG, T. and TOBIASSEN, R. 1995b. Inhibition of endo- and aminopeptidase activities in cytoplasmic fractions of Lactococcus, Lactobacillus and Propionibacterium by peptides from different cheeses. Milchwissenschaft. 50, 565-569.
12. GUIGOZ, Y. and SOLMZ, J. 1974. Isolation of a bitter tasting peptide from "Alpkäse," a Swiss mountain cheese. Lebensm. u. Technol. 7, 356-357.
13. KUNJI, R.S., MIERAU, I., HAGTING, A., POOLMAN, B. and KONINGS, W.K. 1996. The proteolytic systems of lactic acid bacteria. Ant. van Leeuwen. 70, 187-221.
14. LAEMMLI, U.K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 227, 680-685.
15. s1-casein. J. Dairy Res. 60, 401-412.
16. MIERAU, G., TAN, P.S.T., HAANDRIKMAN, A.J., KOK, J., LEENHOUTS, K.J., KONINGS, W.N. and VENEMA, G. 1993. Cloning and sequencing of the gene for a lactococcal endopeptidase, an enzyme with sequence similarity to mammalian enkephalinase. J. Bacteriol. 175, 2087-2096.
17. MONNET, V., NARDI, M., CHOPIN, A., CHOPIN, M.C. and GRIPON, J.C. 1994. Biochemical and genetic characterization of the PepF, an oligopeptidase from Lactococcus lactis. J. Biol. Chem. 51, 32070-32076.
18. MORIHARA, K. 1974. Comparative specificity of microbial proteases. Adv. Enzymol. 41, 179-243.
19. MUSET, G., MONNET, V. and GRIPON, J.C. 1989. Intracellular proteinase of Lactococcus lactis NCDO 763. J. Dairy Res. 56, 765-778.
20. NIVEN, G. 1991. Purification and characterization of aminopeptidase A from Lactococcus lactis subsp. lactis NCDO 712. J. Gen. Microbiol. 137, 1207-1212.
21. NOVAK, P., RAY, P.H. and DEV, I.K. 1986. Localization and purification of two enzymes from Escherichia coli capable of hydrolyzing a signal peptide. J. Biol. Chem. 261, 420-427.
22. ORLOWSKI, M. and WILK, S. 1981. Purification and specificity of a membrane-bound metalloendopeptidase from bovine pituitaries. Biochemistry 20, 4942-4950.
23. PRITCHARD, G.G. and COOLBEAR, T. 1993. The physiology and biochemistry of the proteolytic system in lactic acid bacteria. FEMS Microbiol. Rev. 12, 179-206.
24. SASAKI, M., BOSMAN, B.W. and TAN, P.S.T. 1995. Comparison of proteolytic activities in various lactobacilli. J. Dairy Res. 62, 601-610.
25. SORHAUG, T. and STEPANIAK, L. 1991. Microbial enzymes in the spoilage of milk and dairy products. In Food Enzymology, Vol. 1, (P.F. Fox, ed.) pp. 169-218, Elsevier Applied Science, London.
26. STEPANIAK, L. and FOX, P.F. 1995. Characterization of the principal intracellular endopeptidase from Lactococcus lactis subsp. lactis MG1363. Intern. Dairy J. 5, 699-713.
27. STEPANIAK, L., FOX, P.F., SØRHAUG, T. and GRABSKA, J. 1995. Effect of peptides from the sequence 58-72 of β-casein on the activity of endopeptidase, aminopeptidase, and X-prolyl-dipeptidyl aminopeptidase from Lactococcus lactis spp. lactis MG1363. J. Agric. Food Chem. 43, 849-853.
28. STEPANIAK, L., FOX, P.F., SØRHAUG, T., SLETTEN, K. and TOBIASSEN, R. 1993. Inhibition of endopeptidase from Lactococcus by peptides present in cheese. Proc. Progress in Food Fermentation, Vol. 1, (C. Benedito de Barber, C. Collar, M.A. Martinez-Anaya and J. Morell, eds.) pp. 385-390, Valencia, Spain.
29. STEPANIAK, L., GOBBETTI, M., SORHAUG, T., FOX, ,P.F. and HØJRUP, P. 1996. Peptides inhibitory to endopeptidase and aminopeptidase from Lactococcus lactis ssp. lactis MG1363, released from bovine β-casein by chymosin, trypsin or chymotrypsin. Z. Lebensm. Unters. Forsch. 202, 329-333.
30. TAN, P.S.T., POS, K.M. and KONINGS, W.N. 1991. Purification and characterization of an endopeptidase from Lactococcus lactis subsp. cremoris Wg2. Appl. Environ. Microbiol. 57, 3593-3599.
31. TWINNING, S. 1984. Fluorescein isothiocyanate-labeled casein assay for proteolytic enzymes. Anal. Biochem. 143, 30-34.
32. VIMR, E.C., GREE, L. and MILLERC, C.G. 1983. Oligopeptidase-deficient mutants of Salmonella typhimurium. J. Bacteriol. 153, 1259, 1265.
33. YAN, T.R., AZUMA, N., KAMINOGAWA, S. and YAMAUCHI, K. 1987a. Purification and characterization of a novel metalloendopeptidase from Streptococcus cremoris H61. Eur. J. Biochem. 163, 259-265.
34. YAN, T.R., AZUMA, N., KAMINOGAWA, S. and YAMAUCHI, K. 1987b. Purification and characterization of a substrate-size-recognizing metalloendopeptidase from Streptococcus cremoris H61. Appl. Environ. Microbiol. 53, 2296-2302.