Sildenafil, a type-5 CGMP phosphodiesterase inhibitor, specifically amplifies endogenous cGMP-dependent relaxation in rabbit corpus cavernosum smooth muscle in vitro

Journal of Urology

Volumn 160, Issue 1, 1998, Pages 257-261

  Chuang, Alex T ^a   Strauss, John D ^a   Murphy, Richard A ^a   Steers, William D ^a  

^a UNIVERSITY OF VIRGINIA HEALTH SYSTEM   (United States)

Author keywords

Cyclic GMP; Impotence; Penis; Phosphodiesterase inhibitor; Relaxation

Indexed keywords

CYCLIC GMP; MYOSIN LIGHT CHAIN; NITRIC OXIDE; NITROPRUSSIDE SODIUM; PHENYLEPHRINE; PHOSPHODIESTERASE INHIBITOR; SILDENAFIL;

ANIMAL TISSUE; ARTICLE; CONTROLLED STUDY; CORPUS CAVERNOSUM; DRUG ACTIVITY; ERECTILE DYSFUNCTION; IMPOTENCE; IN VITRO STUDY; MALE; NONHUMAN; PENIS ERECTION; PHOSPHORYLATION; PRIORITY JOURNAL; RABBIT; SIGNAL TRANSDUCTION; SMOOTH MUSCLE; SMOOTH MUSCLE RELAXATION;

EID: 0032323162     PISSN: 00225347     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0022-5347(01)63100-8     Document Type: Article

References (26)

1. Lue, T. F. and Tanagho E. A.: Functional anatomy of penile erection. In: Contemporary Management of Impotence and Infertility. Edited by Tanagho E. A., Lue T. F., and McClure R. D. Baltimore: Williams and Wilkins, pp. 39-50, 1988.
2. Andersson, K.-E.: Pharmacology of lower urinary tract smooth muscles and penile erectile tissues. Pharmacol. Rev., 45: 253, 1993.
3. Andersson, K.-E. and Wagner G.: Physiology of penile erection. Physiol. Rev., 75: 191, 1995.
4. Holmquist, F., Hedlund, H. and Andersson, K.-E.: Characterization of inhibitory neurotransmission in the isolated corpus cavernosum from rabbit and man. J. Physiol. (Lond. ), 449: 295, 1992.
5. Bush, P. A., Aronson, W. J., Buga, G. M., Rajfer, J. and Ignarro, L. J.: Nitric oxide is a potent relaxant of human and rabbit corpus cavernosum. J. Urol., 147: 1650, 1992.
6. Ignarro, L. J., Bush, P. A., Buga, G. M., Wood, K. S., Fukuto, J. M. and Rajfer, J.: Nitric oxide and cyclic GMP formation upon electrical field stimulation cause relaxation of corpus cavernosum smooth muscle. Biochem. Biophys. Res. Commun., 170: 843, 1997.
7. Kim, Y. C., Kim, J. H., Davies, M. G., Hagen, P. O. and Carson, C. C. I.: Modulation of vasoactive intestinal polypeptide (VIP)-mediated relaxation by nitric oxide and prostanoids in the rabbit corpus cavernosum. J. Urol., 153: 807, 1995.
8. Kim, Y. C., Choi, H. K., Ahn, Y. S., Kim, K. H., Hagen, P. O. and Carson, C. C. I.: The effect of vasoactive intestinal polypeptide (VIP) on rabbit cavernosal smooth muscle contractility. J. Androl., 15: 392, 1994.
9. Sparwasser, C., Drescher, P., Will, J. A. and Madsen, P. O.: Smooth muscle tone regulation in rabbit cavernosal and spongiosal tissue by cyclic AMP- and cyclic GMP-dependent mechanisms. J. Urol., 152: 2159, 1994.
10. Bowman, A. and Drummond, A. H.: Cyclic GMP mediates neurogenic relaxation in the bovine retractor penis muscle. Br. J. Pharmacol., 81: 665, 1984.
11. Lincoln, T. M., Komalavilas, P. and Cornwell, T. L.: Pleiotropic regulation of vascular smooth muscle tone by cyclic GMP-dependent protein kinase. Hypertension, 23: 1141, 1994.
12. Boolell, M., Allen, M. J., Ballard, S., Gepi-Attee, S., Muirhead, G. J., Naylor, A. M., Osterloh, I. H. and Gingell, C.: Sildenafil: an orally active type 5 cyclic GMP-specific phosphodiesterase inhibitor for the treatment of penile erectile dysfunction. Int. J. Impot. Res., 8: 47, 1996.
13. Goldstein, I., Lue, T., Padma-Nathan, H., Rosen, R., Steers, W. and Wicker, P.: Oral sildenafil in the treatment of erectile dysfunction. NEJM, in press 1998.
14. Strauss, J. D. and Murphy, R. A.: Regulation of cross-bridge cycling in smooth muscle. In: Biochemistry of Smooth Muscle Contraction. Edited by Bárány, M. New York: Academic Press, Inc., pp. 341-353, 1996.
15. i and uncoupling stress from myosin phosphorylation. Am. J. Physiol. Cell Physiol., 263: C461, 1992.
16. Van Riper, D. A., McDaniel, N. L. and Rembold, C. M.: Myosin light chain kinase phosphorylation in nitrovasodilator induced swine carotid artery relaxation. Biochem. Biophys. Acta Mol. Cell Res., 1355: 323, 1997.
17. de Lanerolle, P., Strauss, J. D., Felsen, R., Doerman, G. E. and Paul, R. J.: Effects of antibodies to myosin light chain kinase on contractility and myosin phosphorylation in chemically permeabilized smooth muscle. Circ. Res., 68: 457, 1991.
18. Matthews, J. N. S., Altman, D. G., Campbell, M. J. and Royston, P.: Analysis of serial measurements in medical research. Br. Med. J., 300: 230, 1990.
19. Lerner, S. E., Melman, A. and Christ, G. J.: A review of erectile dysfunction: new insights and more questions. J. Urol., 149: 1246, 1993.
20. Burnett, A. L., Lowenstein, C. J., Bredt, D. S., Chang, T. S. K. and Snyder, S. H.: Nitric oxide: a physiologic mediator of penile erection. Science, 257: 401, 1992.
21. Virag R.: Intracavernous injection of papaverine for erectile failure. Lancet II: 938, 1982.
22. G-nitro arginine inhibits non-adrenergic, non-cholinergic relaxation of human isolated corpus cavernosum. Acta Physiol. Scand., 141: 441, 1991.
23. Jeremy, J. Y., Ballard, S. A., Naylor, A. M., Miller, M. A. W. and Angelili, G. D.: Effects of sildenafil, a type-5 cGMP phosphodiesterase inhibitor, and papaverine on cyclic GMP and cyclic AMP levels in the rabbit corpus cavernosum in vitro. Br. J. Urol., 79: 958, 1997.
24. Filo, R. S., Bohr, D. F. and Rüegg, J. C.: Glycerinated skeletal and smooth muscle: calcium and magnesium dependence. Science, 147: 1581, 1965.
25. Zhao, W. and Christ, G. J.: Endothelin-1 as a putative modulator of erectile dysfunction. II. Calcium mobilization in cultured human corporal smooth muscle cells. J. Urol., 154: 1571, 1995.
26. Taher, A., Meyer, M., Stief, C. G., Jonas, U. and Forssmann, W. G.: Cyclic nucleotide phosphodiesterase in human cavernous smooth muscle. World J. Urol., 15: 32, 1997.