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85030366335
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note
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Absorption of nucleic acids to the walls of the tubes has only been observed for sample reaction times greater than 2 min. The acquisition of time points on the order of minutes is not required for progress curves with millisecond time scales. However, because parts of the Tetrahymena ribozyme fold with time scales on the order of minutes, the acquisition of slow folding data with the rapid mixer is desirable in order to assure the consistency of the fast and slow folding progress curves. Unpublished data have established the consistency of manual and rapid mixing methods for reactions such as the slow folding of the core of the Tetrahymena ribozyme.
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43
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85030368430
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note
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Beam scattering can also be minimized by the insertion of an argon-filled or vacuum "flight tube" in the path of the beamline in situations where the presence of other equipment precludes placing the shutter and mixer close to the front port.
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46
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85030361301
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note
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The sample holder can be a temperature-regulated block in order to conduct experiments at temperatures other than ambient.
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47
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85030360523
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The -OH cleavage reaction may need to be quenched depending on the time scale of the transition, see earlier. Control experiments for the RNA folding reaction conducted with and without a thiourea quench solution in syringe B were indistinguishable (unpublished data)
-
The -OH cleavage reaction may need to be quenched depending on the time scale of the transition, see earlier. Control experiments for the RNA folding reaction conducted with and without a thiourea quench solution in syringe B were indistinguishable (unpublished data).
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48
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85030362895
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85030361741
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note
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9
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85030360283
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note
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The ImageQuant software is integrated with the Microsoft spreadsheet. Other analysis programs can be substituted through the implementation of the appropriate export and conversion functions.
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