메뉴 건너뛰기




Volumn 10, Issue 18, 1998, Pages 1536-1540

Bioelectronic glasses: Electrical addressability of sol-gel immobilized biomolecules

Author keywords

[No Author keywords available]

Indexed keywords

ENZYMES; MOLECULAR STRUCTURE; POROUS MATERIALS; PROTEINS; REDOX REACTIONS; SOL-GELS;

EID: 0032309725     PISSN: 09359648     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1521-4095(199812)10:18<1536::AID-ADMA1536>3.0.CO;2-4     Document Type: Article
Times cited : (10)

References (25)
  • 15
    • 85034469686 scopus 로고    scopus 로고
    • note
    • 2O in 4 mL of deionized water followed by the addition of 1.55 g of tetramethyl orthosilicate (TMOS). The mixture was sonicated for 15 min to obtain sol. The freshly prepared sol (1 mL) was mixed with the same amount of deionized water and poured into polystyrene cuvettes. Gelation takes place in ca. 2 days. These sol-gels were allowed to age for 2-4 days.
  • 16
    • 85034463212 scopus 로고    scopus 로고
    • note
    • Monolithic configurations were obtained by the formation of sol-gels in standard 4 mL polystyrene cuvettes. Films were formed by spin coating sol onto glass microscope slides. Typical film thickness was ca. 150 μm. The slides were first cleaned with water. The clean slides were then used for depositing films.
  • 18
    • 85034475629 scopus 로고    scopus 로고
    • note
    • The samples containing FMN as a dopant were prepared by first making sol as described earlier [15]. The sol-gels containing FMN were obtained by mixing 2 mL of freshly prepared sol with 0.67 mL of deionized water and 0.25 mL of 0.001 M aqueous solution of FMN. The mixture was poured into a cuvette and covered with parafilm. Gelation takes place in about 4 days. These sol-gels were allowed to age for 1 week under ambient conditions prior to use.
  • 20
    • 85034470329 scopus 로고    scopus 로고
    • note
    • 2O in 3 mL of acetate buffer (0.1 M, pH 4.6) followed by the addition of 1.55 g of TMOS. The mixture was sonicated for 10 min. To 0.5 mL of this sol, 20 μL of 8.25 M NaOH solution was added followed by the addition of 0.1 mL of acetate buffer (pH 4.6). To this mixture, 45 μL of 0.05 M FMN solution was added. Finally, 100 μL of polyvinyl alcohol (2.5 wt.-% in water) was added to enhance the mechanical properties of the films. For film formation, 100 μL of the mixture was used on a clean glass slide.
  • 21
    • 85034484079 scopus 로고    scopus 로고
    • note
    • 3.


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.