Purification and characterization of avian glycolipid: β-Galactosyltransferases (GalT-4 and GalT-3): Cloning and expression of truncated βGalT-4

Acta Biochimica Polonica

Volumn 45, Issue 2, 1998, Pages 451-467

  Basu, Shib S ^b   Dastgheib, Sara ^a   Ghosh, Sujoy ^c   Basu, Manju ^a   Kelly, Patrick ^a   Basu, Subhash ^a  

^a UNIVERSITY OF NOTRE DAME   (United States)

^b DUKE UNIVERSITY SCHOOL OF MEDICINE   (United States)

^c GLAXOSMITHKLINE   (United States)

Author keywords

Cloning of galactosyltransferases; Galactosyltransferases; Ganglioside biosynthesis; Glycolipid biosynthesis; Glycolipid biosynthesis regulation; Glycolipid glycosyltransferases; Glycosyltransferases

Indexed keywords

ANIMALIA; AVES; GALLUS GALLUS;

COMPLEMENTARY DNA; GALACTOSYLTRANSFERASE; GLYCOLIPID;

ANIMAL; ARTICLE; CHICKEN; GENETICS; HUMAN; ISOLATION AND PURIFICATION; MOLECULAR CLONING; PLASMID;

ANIMALS; CHICKENS; CLONING, MOLECULAR; DNA, COMPLEMENTARY; GALACTOSYLTRANSFERASES; GLYCOLIPIDS; HUMANS; PLASMIDS;

EID: 0032254036     PISSN: 0001527X     EISSN: None     Source Type: Journal    
DOI: 10.18388/abp.1998_4239     Document Type: Article

References (67)

1. Roseman, S. (1970) The synthesis of complex carbohydrates by multiglycosyltransferase systems and their potential function in intracellular adhesion. Chem. Phys. Lipids 5, 270-297.
2. Schachter, H. & Roseman, S. (1980) Mammalian glycosyltransferases. Their role in the synthesis and function of complex carbohydrates and glycolipids; in The Biochemistry of Glycoproteins and Proteoglycans (Lennarz, W.J., ed.) pp. 85-160, Plenum Press, New York.
3. Basu, S., Moscal, J.R. & Gardner, D.A. (1976) Scanning electron microscopic and glycosphingolipid biosynthetic studies of differentiating mouse neuroblastoma cells. Symposium Volume on "Biochemical and Pharmacological Implications of Ganglioside Function". (Porcellati, G., ed.) vol. 71, pp. 45-64, Plenum Press, New York.
4. Basu, S., Basu, M., Chien, J.-L. & Prosper, K.A. (1980) Biosynthesis of gangliosides in tissues; in Structure and Function of Gangliosides (Svennerholm, L., Dreyfus, H. & Urban, P.-F., eds.) pp. 213-226, Plenum Publishing Corp.
5. Basu, S. & Basu, M. (1982) Expression of glycosphingolipid glycosyltransferases in development and transformation; in The Glycoconjugates (Horowith, M., ed.) vol. 3, pp. 265-285, Academic Press, New York.
6. Basu, M., De, T., Das, K.K., Kyle, J.W., Chon, H.C., Schaeper, R.J. & Basu, S. (1987) Glycosphingolipids. Methods Enzymol. 138, 575-607.
7. Basu, S., Das, K., Schaeper, R.J., Banerjee, P., Daussin, F., Basu, M., Khan, F.A. & Zhang, B.J. (1988) Biosynthesis in vitro of neuronal and non-neuronal gangliosides; in New Trends in Ganglioside Research: Neurochemical and Neuroregenerative Aspects (Ledeen, R., ed.) pp. 259-273, Fidia Research Series, Italy.
8. Basu, S., Schultz, A., Basu, M. & Roseman, S. (1971) Enzymatic synthesis of galactocerebroside by a galactosyltransferase from embryonic chicken brain J. Biol. Chem. 243, 4272-4279.
9. Basu, S., Basu, M. & Basu, S.S. (1995) Biological specificity of sialyltransferase; in Biology of Sialic Acids (Rosenberg, A., ed.) chapter 3, pp. 69-94, Plenum Press, New York.
10. Basu, S., Basu, M., Dastgheib, S., Hawes, J.W. (1998) Biosynthesis and regulation of glycosphingolipids; in Comprehensive Natural Products Chemistry (Barton, D.H.R., Nakanishi, K. & Meth-Cohn, O., eds.) vol. 3. Carbohydrate, Pergamon Press (in press).
11. Kaufman, B., Basu, S. & Roseman, S. (1967) Studies on the biosynthesis of gangliosides; in Inborn Disorders of Sphingolipid Metabolism (Aronson, S.M. & Volk, B.W., eds.) pp. 193-213, Pergamon Press, New York.
12. Kishimoto, Y. (1983) Sphingolipid formation; in The Enzymes (Boyer, R.D., ed.) vol. 16, pp. 357-407, Academic Press, New York.
13. van Echten, G. & Sandhoff, K. (1993) Ganglioside metabolism: Enzymology, topology and regulation. J. Biol. Chem. 268, 5342-5344.
14. Jungalwala, F.B. (1994) Expression and biological functions of sulfoglucuronyl glycolipids the nervous system - a review. Neurochem. Res. 19, 945-957.
15. Broadbeck, U., Denton, W.L., Tanahashi, N. & Ebner, K.E. (1967) The isolation and identification of the B protein of lactose synthetase as α-lactalbumin. J. Biol. Chem. 242, 1391-1397.
16. Trayer, I.P. & Hill, R.L. (1971) The purification and properties of the A protein of lactose synthase. J. Biol. Chem. 246, 6666-6675.
17. Fujita-Yamaguchi, Y. & Yoshida, A. (1981) Purification and characterization of human serum galactosyltransferase (lactose synthase A protein). J. Biol. Chem. 256, 2701-2706.
18. Basu, M. & Basu, S. (1972) Enzymatic synthesis of a tetraglycosyl-ceramide by a galactosyltransferase from rabbit bone marrow. J. Biol. Chem. 247, 1489-1495.
19. Basu, M. (1972) Study of the biosynthesis of a blood group B specific glycosphingolipid, D.Sc. Thesis, University of Calcutta, Calcutta.
20. Moskal, J. (1977) Structure of a pentaglycosyltransferase from bovine erythrocytes and its biosynthesis in vitro in cultured cells and serum. Ph.D. Thesis, University of Notre Dame, Notre Dame.
21. Basu, S., Basu, M., Moskal, J.R., Chien, J.L. & Gardner, D.A. (1976) Biosynthesis in vitro of neutral glycosphingolipids in normal tissues and cultured cells; in Glycolipid Methodology (Witting, L.A., ed.) pp. 123-139, American Oil Chemists' Society Press, Champaign, IL.
22. Moskal, J.R., Chien, J.L., Basu, M. & Basu, S. (1975) Structure and biosynthesis in vitro of bovine erythrocyte pentaglycosylceramide. Federation Proc. 34, 645.
23. M2 ganglioside, β1-3 galactosyltransferase) from embryonic chicken brain. Glycoconjugate J. 12, 838-847.
24. Ghosh, S. (1992) Studies on the regulation of two galactosyltransferases from embryonic chicken brain, Ph.D. Thesis, University of Notre Dame, Notre Dame.
25. Kyle, J. (1985) Characterization of glycolipid galactosyltransferases from embryonic chicken brains. Ph.D. Thesis, University of Notre Dame, Notre Dame.
26. Basu, S., Basu, M., Kyle, J.W., De, T., Das, K. & Schaper, R.J. (1986) Biosynthesis of gangliosides and blood group glycolipids using solubilized glycosyltransferases; in Enzymes of Lipid Metabolism (Freysz, L. & Gatt, S., eds.) pp. 232-245, Plenum Press, New York.
27. Jenis, D.M. (1981) Levels of galactosyltransferase activities in model tumor systems. Ph.D. Thesis, University of Notre Dame, Notre Dame.
28. Jenis, D.M., Basu, S. & Pollard, M. (1982) Increased activity of a β-galactosyltransferase in tissues of rats bearing prostate and mammary adenocarcinomas. Cancer Biochem. Biophys. 6, 37-45.
29. Moskal, J.R., Gardner, D.A. & Basu, S. (1974) Changes in glycolipid glycosyltransferases and glutamate decarboxylase and their relationship to differentiation in neuroblastoma cells. Biochem. Biophys. Res. Commun. 61, 751-758.
30. Basu, M., Rossi, F., Young, N.M. & Basu, S. (1996) Glycolipid biosynthesis in human neuroblastoma IMR-32 cells. Glycoconjugate J. 6, 723.
31. X by α1-3 fucosyltransferases from colon carcinoma cells and embryonic brain tissues. Glycobiology 1, 527-535.
32. Kaufman, B. & Basu, S. (1966) Embryonic chicken brain sialyltransferase. Methods Enzymol 8, 365-368.
33. X glycolipids in metastatic human colon carcinoma cells. Ind. J. Biochem. Biophys. 30, 324-332.
34. Holmes, E. (1989) Characterization and membrane organization of β1-3 and β1-4 GalTs, from human Colo-205 and SW 403. Arch. Biochem. Biophys. 270, 630-646.
35. Holmes, E.H. (1992) Brefel din A induced inhibition of de novo globo- and neolacto-series glycolipid core chain biosynthesis in human cells. J. Biol Chem. 267, 25328-25336.
36. Basu, M., Basu, S. & Potter, M. (1980) Biosynthesis of blood group related glyco-sphingolipids in T- and B-lymphomas and neuroblastoma cells; in Cell Surface Glycolipids (Sweeley, C.C., ed.) vol. 128, 187-212, American Chemical Society Symposium.
37. Basu, M., Weng, S.-A., Tang, H., Khan, F., Zhang, B.J. & Basu, S. (1996) Biosynthesis in vitro of neolactotetraosylceramide by a galactosyltransferase from mouse T-lymphoma. Purification and kinetic studies; Synthesis of neolacto and polylactosamine core. Glycoconjugate J. 13, 423-432.
38. Khan, F.A., Basu, M., Das, K.K., Zhang, B., Graber, K.A., Suzuki, I. & Basu, S. (1988) Glycolipid:GalT-4 from mouse T-lymphoma and human colon carcinoma-purification and comparative studies. J. Cell Biol. 107, 209a.
39. Shaper, N.L., Shaper, J.H., Meuth, J.L., Fox, L., Chang, H., Kirsch, I.R. & Hollis, G.F. (1986) Bovine galactosyltransferase: Identification of a clone by direct immunological screening of a cDNA expression library. Proc. Natl. Acad. Sci. U.S.A. 83, 1573-1577.
40. Appert, H.E., Rutherford, T.J., Tarr, G.E., Wiest, J.S., Thomford, N.R. & McCorquodale, D.J. (1986) Isolation of a cDNA coding for human galactosyltransferase. Biochem. Biophys. Res. Commun. 139, 163-168.
41. Nakazawa, K., Ando, T., Kimura, T. & Narimatsu, H. (1988) Cloning and sequencing of a full-length cDNA of mouse N-acetylglucosamine (β1-4)galactosyltransferase. J. Biochem. (Tokyo) 104, 165-168.
42. Shaper, N.L., Hollis, G.F., Douglas, J.G., Kirsch, I.R. & Shaper, J.H. (1988) Characterization of the full length cDNA for murine β1-4 galactosyltransferase: Novel features at the 5′ end predict two translational start sites at two in frame AUGs. J. Biol. Chem. 263, 10420-10428.
43. Joziasse, D.H., Shaper, J.H., van den Eijnden, E.H., van Tunen, A.J. & Shaper, N.L. (1989) Bovine α1-3 galactosyltransferase: Identification and characterization of a cDNA clone. Identification of homologous sequences in human genomic DNA. J. Biol. Chem. 264, 14290-14297.
44. Ghosh, S., Basu, S.S. & Basu, S. (1992) Isolation of cDNA clone for β1-4galactosyltransferase from embryonic chicken brain and comparison to its mammalian homologs. Biochem. Biophys. Res. Commun. 189, 1215-1222.
45. Basu, S., Ghosh, S., Basu, S.S., Kyle, J.W., Li, T. & Basu, M. (1993) Regulation of expression of cell surface neolactoglycolipids and cloning of embryonic chicken brain GalT-4. Ind. J. Biochem. Biophys. 30, 315-323.
46. Ghosh, S., Basu, S.S., Strum, J.C., Basu, S. & Bell, R.M. (1995) Identification of conditions that facilitate the expression of GST-fusions as soluble, full-length proteins. Anal. Biochem. 225, 376-378.
47. Basu, S.S. (1996) Cloning and expression of two glycolipid:glycosyltransferases, GalT-4 and SAT-3. Ph.D. Thesis, University of Notre Dame, Notre Dame.
48. Basu, S., Kaufman, B. & Roseman, S. (1965) Conversion of Tay-Sachs ganglioside to monosialoganglioside by brain uridine diphosphate D-galactose: Glycolipid galactosyltransferase. J. Biol. Chem. 240, 4114-4117.
49. Yip, G.B. & Dain, J.A. (1970) The enzymatic synthesis of ganglioside. II. UDP-galactose: N-acetylgalactosaminyl (-N-acetyl-neuraminyl) galactosyl glucosyl ceramide galactosyltransferase in rat brain. Biochim. Biophys. Acta 206, 252-260.
50. M1 synthetase activity on intact chick neural retinal cells. J. Cell. Biol. 93, 76-81.
51. Furukawa, K. & Roth, S. (1985) Co-purification of galactosyltransferases from chick embryo liver. Biochem. J. 227, 573-582.
52. M2 ganglioside from rat liver. J. Biol. Chem. 258, 770-776.
53. Basu, M., Presper, K.A., Basu, S., Hoffman, L.M. & Brooks, S.E. (1979) Differential activities of glycolipid glycosyltransferase in Tay-Sachs disease: Studies in cultured cells from cerebrum. Proc. Natl. Acad. Sci., U.S.A. 76, 4270-4274.
54. M2 β1:3galactosyltransferase from embryonic chicken brains). Glycoconjugate J. 5, S48.
55. Ghost, S., Li, Z., Basu, M. & Basu, S. (1992) Separation and purification of two galactosyltransferase activities from embryonic chicken brain and their inhibition by the anion transport inhibitor, DIDS. FASEB J. 6, A492.
56. M2β1-3galactosyltransferase) activity from embryonic chicken brain. Ind. J. Biochem. Biophys. 27, 379-385.
57. Das, K., Base, S. & Basu, M. (1984) A rapid preparative method for isolation of neutral and acidic glycosphingolipids by radial thin layer chromatography. Anal. Biochem. 125-134.
58. Basu, S., Basu, M., Das, K.K., Daussin, F., Schaeper, R.J., Banerjee, P. Khan, F.A. & Suzuki, I. (1988) Solubilized glycosyltransferases and biosynthesis in vitro of glycolipids. Biochimie 70, 1551-1563.
59. Merril, C.R., Goldman, D.A., Sedman, S.A. & Ebert, M.M. (1981) Ultrasensitive stain for proteins in polyacrylamide gels show regional variations in cerebrospinal fluid proteins. Science 211, 1437-1438.
60. Cleveland, D.W., Fischer, S.G., Kirschner, M.W. & Leammli, U.K. (1977) Peptide mapping by limited proteolysis in sodium dodecyl sulfate and analysis by gel electrophoresis. J. Biol. Chem. 252, 1102-1106.
61. Habib, W.H. & Jacoby, W.B. (1981) Assays of differentiation of glutathione β-transferase. Methods Enzymol. 77, 398-405.
62. Zielenski, J. & Koscielak, J. (1982) Enzymatic synthesis of neolactotetraosylceramide by the N-acetyllactosamine synthase of human serum. Eur. J. Biochem. 125, 323-329.
63. Dreyfus, H., Urban, P.F., Edel-Harth, S. & Mandel, P. (1975) Developmental patterns of gangliosides and phospholipids in chicken retina and brain. J. Neurochem. 25, 245-250.
64. Rosner, H. (1975) Changes in the contents of gangliosides and glycoproteins and in the ganglioside pattern of chicken brain, J. Neuro. Chem. 24, 815-816.
65. Ghosh, S., Lee, S., Brown, T.A., Basu, M., Hawes, J.W., Davidson, D.A. & Basu, S. (1991) Use of exoglycosidases from M. mercenaria (hard shell clam) as a tool for structural studies of glycosphingolipids and glycoproteins. Anal. Biochem. 196, 252-261.
66. Dastgheib, S. (1997) Characterization of β(1-3)galactosyltransferase and ceramide glycanase involved in biosynthesis and degradation of gangliosides. Ph.D Thesis, University of Notre Dame, Notre Dame.
67. M1/GA1. J. Biol. Chem. 272, 24794-24799.