Detection of non-covalent interaction of single and double stranded DNA with peptides by MALDI-TOF

Proteins: Structure, Function and Genetics

Volumn 33, Issue SUPPL. 2, 1998, Pages 12-21

  Lin, Shanhua ^a   Cotter, Robert J ^a   Woods, Amina S ^a  

^a JOHNS HOPKINS UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

DNA; Non covalent interaction; Peptides

Indexed keywords

DNA BINDING PROTEIN; DOUBLE STRANDED DNA; OLIGONUCLEOTIDE; SINGLE STRANDED DNA; SYNTHETIC PEPTIDE; DNA; OLIGODEOXYRIBONUCLEOTIDE; PEPTIDE; QUATERNARY AMMONIUM DERIVATIVE;

AMINO ACID SEQUENCE; ANALYTIC METHOD; ARTICLE; IONIC STRENGTH; MASS SPECTROMETRY; PEPTIDE ANALYSIS; PH; PRIORITY JOURNAL; PROTEIN DNA INTERACTION; X RAY CRYSTALLOGRAPHY; METABOLISM; METHODOLOGY;

DNA; DNA, SINGLE-STRANDED; HYDROGEN-ION CONCENTRATION; OLIGODEOXYRIBONUCLEOTIDES; PEPTIDES; QUATERNARY AMMONIUM COMPOUNDS; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION;

EID: 0032253727     PISSN: 08873585     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1097-0134(1998)33:2+<12::AID-PROT3>3.0.CO;2-C     Document Type: Article

References (10)

1. Tang, X., Callahan, J.H., Zhou, P., Vertes, A. Noncovalent protein-oligonucleotide interactions monitored by matrix-assisted laser desorption/ionization mass spectrometry. Anal. Chem. 67:4542-4548, 1995.
2. Lecchi, P., Pannell, L.K. The detection of intact double-stranded DNA by MALDI. J. Am. Soc. Mass. Spectrom. 6:972-975, 1995.
3. Isenberg, I. Histones. Annu. Rev. Biochem. 48:159-191, 1979.
4. McGhee, J.D., Felsenfeld, G. Nucleosome structure. Annu. Rev. Biochem. 49:1115-1156, 1980.
5. Woods, A.S., Buchsbaum, J.C., Worrall, T.A., Cotter, R.J. Matrix-assisted laser desorption/ionization of noncovalently bound compounds. Anal. Chem. 67:4462-4465, 1995.
6. Juhasz, P., Biemann, K. Mass spectrometric molecular-weight determination of highly acidic compounds of biological significance via their complexes with basic polypeptides. Proc. Natl. Acad. Sci. USA 91:4333-4337, 1994.
7. Bull, H.B., Breese, K. Surface tension of amino acid solutions: A hydrophobicity scale of the amino acid residues. Arch. Biochem. Biophys. 161:665-670, 1974.
8. Cheng, X., Harms, A.C., Goudreau, P.N., Terwilliger, T.C., Smith, R.D. Direct measurement of oligonucleotide binding stoichiometry of gene V protein by mass spectrometry. Proc. Natl. Acad. Sci. USA 93:7022-7027, 1996.
9. Cheng, X., Morin, P.E., Harms, A.C., Bruce, J.E., Ben-David, Y., Smith, R.D. Mass spectrometric characterization of sequence-specific complexes of DNA and transcription factor PU.1 DNA binding domain. Anal. Chem. 239:35-40, 1996.
10. Hermann T., Meier, T., Götte, M., Heumann, H. The 'helix clamp' in HIV-1 reverse transcriptase: A new nucleic acid binding motif common in nucleic acid polymerases. Nucleic Acids Res. 22:4625-4633, 1994.