Application of Polymerase Chain Reaction for Determination of Toxins in Escherichia coli Strains Isolated from Pigs with Diarrhea

Acta Microbiologica Polonica

Volumn 47, Issue 4, 1998, Pages 409-413

  Osek, Jacek ^a  

^a NATIONAL VETERINARY RESEARCH INSTITUTE   (Poland)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL DNA; BACTERIAL TOXIN; ENTEROTOXIN; SHIGA TOXIN;

ANIMAL; ANIMAL DISEASE; ARTICLE; DIARRHEA; ENTEROBACTER INFECTION; ESCHERICHIA COLI; GENETICS; ISOLATION AND PURIFICATION; METABOLISM; METHODOLOGY; MICROBIOLOGY; POLYMERASE CHAIN REACTION; SWINE; SWINE DISEASE;

ANIMALS; BACTERIAL TOXINS; DIARRHEA; DNA, BACTERIAL; ENTEROTOXINS; ESCHERICHIA COLI; ESCHERICHIA COLI INFECTIONS; POLYMERASE CHAIN REACTION; SHIGA TOXINS; SWINE; SWINE DISEASES;

EID: 0032250513     PISSN: 01371320     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (17)

1. Calderwood S.B., D.W.K. Acheson, G.T. Keusch, T.J. Barret, P.M. Griffin, N.A. Stockbine, B. Swaminathan, J.B. Kaper, M.M. Levine, H. Karch, A.D. O'Brien, T.G. Obrig, Y. Takeda, P.I. Tarr and I.K. Wachsmuth. 1996. Proposed new nomenclature for SLT (VT) family. ASM News, 62: 118-119.
2. Celemin C., P. Rubio, P. Echeverria and S. Suarez. 1995. Gene toxin patterns of Escherichia coli isolated from diseased and healthy piglets. Vet. Microbiol. 45: 121-127.
3. Gannon V.P.J., L.C. Gyles and R.W. Friendship. 1988. Characteristics of verotoxigenic Escherichia coli from pigs. Can. J. Vet. Res. 52: 331-337.
4. Holland R.E. 1990. Some infectious cases of diarrhea in young farm animals. Clin. Microbiol. Rev. 3: 345-375.
5. Imberechts H., H. De Greve and P. Lintermans. 1992. The pathogenesis of edema disease in pigs. Vet. Microbiol. 40: 219-230.
6. Karmali M.A. 1989. Infection by verocytotoxin-producing Escherichia coli. Clin. Microbiol. Rev. 2: 15-38.
7. MacLeod D.L. and C.L. Gyles. 1990. Purification and characterizatioon of an Escherichia coli shiga-like toxin II variant. Infect. Immun. 58: 1232-1239.
8. Moseley S.L., J.W. Hardy, M.I. Huq, P. Echeverria and S. Falkow. 1983. Isolation and nucleotide sequence determination of a gene encoding a heat-stable enterotoxin of Escherichia coli. Infect. Immun. 39: 1167-1174.
9. Nagy B., T.A., Casey T.A. and H.W. Moon. 1990. Phenotype and genotype of Escherichia coli isolated from pigs with postweaning diarrhoea in Hungary. J. Clin. Microbiol. 28: 651-653.
10. O'Brien A.D. and R.K. Holmes. 1987. Shiga and shiga-like toxins. Microbiol. Rev. 51: 206-220.
11. Ojeniyi B., P. Ahrens and A. Meyling. 1994. Detection of fimbrial and toxin genes in Escherichia coli and their prevalence in piglets with diarrhoea. The application of colony hybridization assay, polymerase chain reaction and phenotypic assays. J. Vet. Med. B 41: 49-59.
12. Olive D.M. 1989. Detection of enterotoxigenic Escherichia coli after polymerase chain reaction amplification with termostable DNA polymerase. J. Clin. Microbiol. 27: 261-265.
13. Picken R.N., A.J. Mazatis, W.K. Maas, M. Rey and H. Heyneker. 1983. Nucleotide sequence of the gene for heat-stable enterotoxin II of Escherichia coli. Infect. Immun. 42: 269-275.
14. Sears C.L. and J.B. Kaper. 1996. Enteric bacterial toxins: Mechanisms of action and linkage to intestinal secretion. Microbiol. Rev. 60: 167-215.
15. Woodward M.J. and C. Wray. 1990. Nine DNA probes for detection of toxin and adhesin genes in Escherichia coli isolated from diarrhoeal disease in animals. Vet. Microbiol. 25: 55-65
16. Woodward M.J., P.J. Carroll and C. Wray. 1992. Detection of entero- and verocyto-toxin genes in Escherichia coli from diarrhoeal disease in animals using the polymerase chain reaction. Vet. Microbiol. 31: 251-261.
17. Wray C. and J.A. Morris. 1985. Aspects of colibacillosis in farm animals. J. Hygiene, 95: 577-593.