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Growth in cell length in the fission yeast Schizosaccharomyces pombe
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Mitchison JM, Nurse P: Growth in cell length in the fission yeast Schizosaccharomyces pombe. J Cell Sci 1985, 75:357-376.
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Localization of F-actin through the cell division cycle of Schizosaccharomyces pombe
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Marks J, Hyams JS: Localization of F-actin through the cell division cycle of Schizosaccharomyces pombe. Eur J Cell Biol 1985, 110:417-425.
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Growth polarity and cytokinesis in fission yeast: The role of the cytoskeleton
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Marks J, Hagan IM, Hyams JS: Growth polarity and cytokinesis in fission yeast: the role of the cytoskeleton. J Cell Sci Suppl 1986, 5:229-241.
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Marks, J.1
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The use of cell division cycle mutants to investigate the control of microtubule distribution in the fission yeast Schizosaccharomyces pombe
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Hagan IM, Hyams JS: The use of cell division cycle mutants to investigate the control of microtubule distribution in the fission yeast Schizosaccharomyces pombe. J Cell Sci 1988, 89:343-357.
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Hagan, I.M.1
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The fission yeast microtubule cytoskeleton
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Hagan I: The fission yeast microtubule cytoskeleton. J Cell Sci • 1998, 111:1603-1612. A good review presenting an up-to-date description of the microtubule cytoskeleton in fission yeast and a discussion of its role during the cell cycle.
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Hagan, I.1
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Cell cycle specificity of certain microtubular drugs in Schizosaccharomyces pombe
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Walker GM: Cell cycle specificity of certain microtubular drugs in Schizosaccharomyces pombe. J Gen Microbiol 1982, 128:61-71.
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Walker, G.M.1
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Cold-sensitive nuclear division arrest mutants of the fission yeast Schizosaccharomyces pombe
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Toda T, Umesono K, Hirata A, Yanagida M: Cold-sensitive nuclear division arrest mutants of the fission yeast Schizosaccharomyces pombe. J Mol Biol 1983, 168:251-270.
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Toda, T.1
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8
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0029957226
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Microtubules mediate mitochondrial distribution in fission yeast
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Yaffe MP, Harata D, Verde F, Eddison M, Toda T, Nurse P: Microtubules mediate mitochondrial distribution in fission yeast. Proc Natl Acad Sci USA 1996, 93:11664-11668.
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Proc Natl Acad Sci USA
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Yaffe, M.P.1
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Toda, T.5
Nurse, P.6
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9
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0031817512
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Identification of novel temperature-sensitive lethal alleles in essential beta-tubulin and nonessential alpha2-tubulin genes as fission yeast polarity mutants
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Radcliffe P, Hirata D, Childs D, Vardy L, Toda T: Identification of novel temperature-sensitive lethal alleles in essential beta-tubulin and nonessential alpha2-tubulin genes as fission yeast polarity mutants. Mol Biol Cell 1998, 9:1757-1771.
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Mol Biol Cell
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Radcliffe, P.1
Hirata, D.2
Childs, D.3
Vardy, L.4
Toda, T.5
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10
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0030611667
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Mal3, the fission yeast homologue of the human APC-interacting protein EB-1 is required for microtubule integrity and the maintenance of cell form
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Beinhauer JD, Hagan IM, Hegemann JH, Fleig U: Mal3, the fission • yeast homologue of the human APC-interacting protein EB-1 is required for microtubule integrity and the maintenance of cell form. J Cell Biol 1997, 139:717-728. This paper describes the cloning and characterization of mal3, which encodes a microtubule associated protein, mal3 mutants display shortened microtubules and an abnormal cell shape. Overexpression of Mal3 compromizes the formation of the mitotic spindle. Interestingly, Mal3 is homologous to EB-1, a human protein found to associate with the tumor suppressor protein APC. EB-1 can substitute for loss of Mal3 in fission yeast cells, suggesting that the two proteins might have similar functions.
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J Cell Biol
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Beinhauer, J.D.1
Hagan, I.M.2
Hegemann, J.H.3
Fleig, U.4
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11
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0032472940
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Essential role of tubulin-folding cofactor D in microtubule assembly and its association with microtubules in fission yeast
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Hirata D, Masuda H, Eddison M, Toda T: Essential role of tubulin-folding cofactor D in microtubule assembly and its association with microtubules in fission yeast EMBO J 1998, 17:658-666.
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EMBO J
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Hirata, D.1
Masuda, H.2
Eddison, M.3
Toda, T.4
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12
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0029610382
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Fission yeast cell morphogenesis: Identification of new genes and analysis of their role during the cell cycle
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Verde F, Mata J, Nurse P: Fission yeast cell morphogenesis: identification of new genes and analysis of their role during the cell cycle. J Cell Biol 1995, 131:1-10.
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(1995)
J Cell Biol
, vol.131
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Verde, F.1
Mata, J.2
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13
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0031012576
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A role for the actin cytoskeleton of Saccharomyces cerevisiae in bipolar bud-site selection
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Yang S, Ayscough KR, Drubin DG: A role for the actin cytoskeleton of Saccharomyces cerevisiae in bipolar bud-site selection. J Cell Biol 1997, 136:111-123.
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J Cell Biol
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Yang, S.1
Ayscough, K.R.2
Drubin, D.G.3
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14
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0030815426
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Microtubule function in morphological differentiation: Growth zones and growth cones
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Vega LR, Solomon F: Microtubule function in morphological differentiation: growth zones and growth cones. Cell 1997, 89:825-828.
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Cell
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Vega, L.R.1
Solomon, F.2
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15
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0028285110
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Genetic analysis of cell morphogenesis in fission yeast - A role for casein kinase II in the establishment of polarized growth
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Snell V, Nurse P: Genetic analysis of cell morphogenesis in fission yeast - a role for casein kinase II in the establishment of polarized growth. EMBO J 1994, 13:2066-2074.
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(1994)
EMBO J
, vol.13
, pp. 2066-2074
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Snell, V.1
Nurse, P.2
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16
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0030773728
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Tea1 and the microtubular cytoskeleton are important for generating global spatial order within the fission yeast cell
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Mata J, Nurse P: tea1 and the microtubular cytoskeleton are •• important for generating global spatial order within the fission yeast cell. Cell 1997, 89:939-949. This paper describes the cloning of tea1 and localization of its product both cell tips during the cell cycle. Tea1 is also shown to accumulate preferentially on microtubule ends, and to be dependent on the microtubule cytoskeleton, but not on actin, for localization to the cell tips. Evidence is also presented that suggests a role for Teal in the regulation of microtubule length. Moreover, Tea1 protein levels are shown to decrease during exposure to pheromone; it is suggested that a decrease of Tea1 levels may allow the cell tips to grow following an extracellular pheromone signal.
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Cell
, vol.89
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Mata, J.1
Nurse, P.2
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17
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0040839027
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Pom1p, a fission yeast protein kinase that provides positionalinformation for both polarized growth and cytokinesis
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Bahler J, Pringle JR: Pom1p, a fission yeast protein kinase that •• provides positionalinformation for both polarized growth and cytokinesis. Genes Dev 1998, 12:1356-1370. This paper describes the isolation of pom1 mutants and the cloning of the pom1 gene, pom1 mutants display mislocalization of the growth sites, asymmetric septa and NETO defects, pom1 encodes a putative ser/thr kinase, with similarity to rat Dyrk, S. cerevisiae Yak1, and Drosophila and human Minibrains, which belong to a novel kinase family. Pom1 localizes to both cell tips in most cells, albeit it seems to be more strongly concentrated at the non-growing tip. Its localization is dependent on microtubules but not on actin.
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Genes Dev
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Bahler, J.1
Pringle, J.R.2
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18
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0032572528
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Regulation of cell polarity by microtubules in fission yeast
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Sawin KE, Nurse P: Regulation of cell polarity by microtubules in •• fission yeast. J Cell Biol 1998, 142:457-471. This paper describes in greater detail the process of cell branching induced by the microtubule-depolymerizing drug thiabendazole (TBZ). Interestingly, cell branching is observed at high frequency only when cells have not activated bipolar growth and are therefore in a pre-NETO state, suggesting that microtubules may have an important role during transition from monopolar to bipolar growth. Moreover, microtubule disruption causes redistribution of the Cdc42-associated Scd2/Ral3 protein from the actively growing cell tips to the cell middle, preceding the appearance of actin. Scd2/Ral3 is proposed to be part of the cue that specify the site of branch formation.
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(1998)
J Cell Biol
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, pp. 457-471
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Sawin, K.E.1
Nurse, P.2
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19
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0031890697
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Discovering the poles in yeast
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Mata J, Nurse P: Discovering the poles in yeast. Trends Cell Biol • 1998, 8:163-167. This review compares mechanisms of control of cell morphology in the two yeast, the budding yeast Saccharomyces cerevisiae and the fission yeast Schizosaccharomyces pombe.
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Trends Cell Biol
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Mata, J.1
Nurse, P.2
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20
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0027892957
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Investigations into the control of cell form and polarity: The use of morphological mutants in fission yeast
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Snell V, Nurse P: Investigations into the control of cell form and polarity: the use of morphological mutants in fission yeast. Dev Suppl 1993, 289-299.
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Dev Suppl
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Snell, V.1
Nurse, P.2
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21
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0024206818
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Isolation and characterization of Schizosaccharomyces pombe mutants phenotypically similar to ras1-
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Fukui Y, Yamamoto M: Isolation and characterization of Schizosaccharomyces pombe mutants phenotypically similar to ras1-. Mol Gen Genet 1988, 215:26-31.
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Yamamoto, M.2
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Cooperative interaction of S. pombe proteins required for mating and morphogenesis
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Chang EC, Barr M, Wang Y, Jung V, Xu HP, Wigler MH: Cooperative interaction of S. pombe proteins required for mating and morphogenesis. Cell 1994, 79:131-141.
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Chang, E.C.1
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Jung, V.4
Xu, H.P.5
Wigler, M.H.6
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23
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0028052984
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Cdc42p GTPase is involved in controlling polarized cell growth in Schizosaccharomyces pombe
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Miller PJ, Johnson DI: Cdc42p GTPase is involved in controlling polarized cell growth in Schizosaccharomyces pombe. Mol Cell Biol 1994, 14:1075-1083.
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Miller, P.J.1
Johnson, D.I.2
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24
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0031263586
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The small GTP-binding protein Rho1 is a multifunctional protein that regulates actin localization, cell polarity, and septum formation in the fission yeast Schizosaccharomyces pombe
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Nakano K, Arai R, Mabuchi I: The small GTP-binding protein Rho1 is a multifunctional protein that regulates actin localization, cell polarity, and septum formation in the fission yeast Schizosaccharomyces pombe. Genes Cells 1997, 2:679-694.
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Genes Cells
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Nakano, K.1
Arai, R.2
Mabuchi, I.3
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25
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0031931284
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Genes that cause aberrant cell morphology by overexpression in fission yeast: A role of a small GTP-binding protein Rho2 in cell morphogenesis
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Hirata D, Nakano K, Fukui M, Takenaka H, Miyakawa T, Mabuchi I: • Genes that cause aberrant cell morphology by overexpression in fission yeast: a role of a small GTP-binding protein Rho2 in cell morphogenesis. J Cell Sci 1998, 111:149-159. This paper describes the identification of Rho2 using a screen for genes that cause aberrant cell morphology when overexpressed. Rho2 null mutant cells are mishapen, suggesting a role for Rho2 in the control of cell form. Rho2 is found localized at the growing ends during interphase and the site of septation during mitosis. Reciprocal suppression analysis of Rho1 and Rho2 suggests that their functions are distinct.
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J Cell Sci
, vol.111
, pp. 149-159
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Hirata, D.1
Nakano, K.2
Fukui, M.3
Takenaka, H.4
Miyakawa, T.5
Mabuchi, I.6
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26
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0027212463
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Two novel protein kinase C-related genes of fission yeast are essential for cell viability and implicated in cell shape control
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Toda T, Shimanuki M, Yanagida M: Two novel protein kinase C-related genes of fission yeast are essential for cell viability and implicated in cell shape control. EMBO J 1993, 12:1987-1995.
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Toda, T.1
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0029814062
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Rho 1 GTPase activates the (1-3)beta-D-glucan synthase and is involved in Schizosaccharomyces pombe morphogenesis
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Arellano M, Duran A, Perez P: Rho 1 GTPase activates the (1-3)beta-D-glucan synthase and is involved in Schizosaccharomyces pombe morphogenesis. EMBO J 1996, 15:4584-4591.
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Arellano, M.1
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Perez, P.3
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Localisation of the Schizosaccharomyces pombe rhol p GTPase and its involvement in the organisation of the actin cytoskeleton
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Arellano M, Duran A, Perez P: Localisation of the • Schizosaccharomyces pombe rhol p GTPase and its involvement in the organisation of the actin cytoskeleton. J Cell Sci 1997, 110:2547-2555. This paper describes the localization of Rho1, which is found at the cell tips during interphase and at the site of septation during mitosis. Moreover, Rho1 depletion induces disappearance of actin patches and cell lysis, suggesting a role for Rho1 in the control of the actin cytoskeleton.
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J Cell Sci
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Arellano, M.1
Duran, A.2
Perez, P.3
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Shk1, a homolog of the Saccharomyces cerevisiae Ste20 andmammalian p65PAK protein kinases, is a component of a Ras/Cdc42 signaling module in the fission yeast Schizosaccharomyces pombe
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Marcus S, Polverino A, Chang E, Robbins D, Cobb MH, Wigler MH: Shk1, a homolog of the Saccharomyces cerevisiae Ste20 andmammalian p65PAK protein kinases, is a component of a Ras/Cdc42 signaling module in the fission yeast Schizosaccharomyces pombe. Proc Natl Acad Sci USA 1995, 92:6180-6184.
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Marcus, S.1
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Chang, E.3
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Cobb, M.H.5
Wigler, M.H.6
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30
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0028879613
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Fission yeast pak1+ encodes a protein kinase that interacts with Cdc42p and is involved in the control of cell polarity and mating
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Ottilie S, Miller PJ, Johnson DI, Creasy CL, Sells MA, Bagrodia S, Forsburg SL, Chernoff J: Fission yeast pak1+ encodes a protein kinase that interacts with Cdc42p and is involved in the control of cell polarity and mating. EMBO J 1995, 14:5908-5919.
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Ottilie, S.1
Miller, P.J.2
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Sells, M.A.5
Bagrodia, S.6
Forsburg, S.L.7
Chernoff, J.8
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31
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0032541080
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Characterization of Pak2p, a pleckstrin homology domain-containing, p21-activated protein kinase from fission yeast
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•] describe the cloning of a novel gene pak2/shk2, which encode a second Cdc42/Rac-activated protein kinase homolog in fission yeast. Cdc42 is shown to interact physically with Pak2/Shk2 and to stimulate its kinase activity. pak2/shk2 is not an essential gene and pak2/shk2 null mutants do not display morphological and mating defects. Pak2/Shk2 over-expression does restore viability and normal cell morphology to a pak1/shk1 null mutant, but does not suppress its mating defect, suggesting that these two kinases also have distinct functions.
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J Biol Chem
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Sells, M.A.1
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Cloning and characterization of shk2, a gene encoding a novel p21-activated protein kinase from fission yeast
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Yang P, Kansra S, Pimentai RA, Gilbreth M, Marcus S: Cloning and • characterization of shk2, a gene encoding a novel p21-activated protein kinase from fission yeast. J Biol Chem 1998, 273:18481-18489. In this paper, in addition to characterizing the gene pak2/shk2, the authors show that byr2 overexpression can can substantially increase the mating efficiency of a pak1/shk1 null mutant partially suppressed by pak2/shk2 over-expression, suggesting that Byr2 acts downstream of the Pak1/Shk1 kinase.
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J Biol Chem
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Yang, P.1
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Fission yeast orb6, a ser/thr protein kinase related to mammalian rho kinase and myotonic dystrophy kinase, is required for maintenance of cell polarity and coordinates cell morphogenesis with the cell cycle
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Verde F, Wiley DJ, Nurse P: Fission yeast orb6, a ser/thr protein •• kinase related to mammalian rho kinase and myotonic dystrophy kinase, is required for maintenance of cell polarity and coordinates cell morphogenesis with the cell cycle. Proc Natl Acad Sci USA 1998, 95:7526-7531. This paper describes the cloning of orb6 and its identification as a kinase belonging to the warts/Rho-kinase family. Orb6 is required for maintenance of cell polarity and for correct localization of the actin cytoskeleton. Changes of Orb6 protein levels alter the timing of mitotic onset, suggesting a role for Orb6 in the control of cell cycle progression. Moreover, orb6 is shown to interact genetically with orb2, which encodes the Cdc42-dependent Pak1/Shk1 kinase, indicating that Orb6 may function downstream of the Cdc42-dependent morphology control pathway.
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Proc Natl Acad Sci USA
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Verde, F.1
Wiley, D.J.2
Nurse, P.3
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The highly conserved skb1 gene encodes a protein that interacts with Shk1, a fission yeast Ste20/PAK homolog
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Control of cell size at division in fission yeast by a growth-modulated size control over nuclear division
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Stern B, Nurse P: Fission yeast pheromone blocks S-phase by inhibiting the G1 cyclin B-p34cdc2 kinase. EMBO J 1997, 16:534-544.
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The kinetics of H1 histone kinase activation during the cell cycle of wild-type and wee mutants of the fission yeast Schizosaccharomyces pombe
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Gould KL, Simanis V: The control of septum formation in fission • yeast. Genes Dev 1997, 11:2939-2951. This comprehensive review summarizes the results of the genetical dissection of cytokinesis in fission yeast, which has proven a successful undertaking in the past few years.
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Jwa M, Song K: Byr4, a dosage-dependent regulator of cytokinesis in S. pombe, interacts with a possible small GTPase pathway including Spg1 and Cdc16. Mol Cells 1998, 8:240-245.
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