Thermal profile with alternately raised and lowered annealing temperature improves the PCR amplification using highly degenerate primers

Acta Biochimica Polonica

Volumn 45, Issue 3, 1998, Pages 691-694

  Sachadyn, Paweł ^a   Sobiewska, Gabriela ^a   Kur, Józef ^a  

^a GDANSK UNIVERSITY OF TECHNOLOGY   (Poland)

Author keywords

Degenerate primers; Glucosamine 6 phosphate synthase gene; PCR

Indexed keywords

PRIMER DNA;

ARTICLE; POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; STANDARD; TEMPERATURE;

DNA PRIMERS; POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; TEMPERATURE;

EID: 0032248194     PISSN: 0001527X     EISSN: None     Source Type: Journal    
DOI: 10.18388/abp.1998_4262     Document Type: Article

References (2)

1. von Eggeling, F. & Spielvogel, H. (1995) Applications of random PCR. Cell. Mol. Biol. 41, 653-670.
2. Wesley, C.S., Ben, M., Kreitman, M., Hagag, N. & Eanes, W.F. (1989) Cloning regions of the Drosophila genome by microdissection of polytene chromosome DNA and PCR with non-specific primer. Nucleic Acids Res. 18, 599- 603.