Isolation of human α2HS-glycoprotein synthesized by SF9 cells

Acta Microbiologica et Immunologica Hungarica

Volumn 45, Issue 3-4, 1998, Pages 419-424

  Lorincz Zs ^b   Kalabay, L ^a   Cseh, S ^b   Zavodszky P ^b   Arnaud, P ^c   Jakab, L ^a  

^a SEMMELWEIS UNIVERSITY OF MEDICINE   (Hungary)

^b INSTITUTE OF ENZYMOLOGY   (Hungary)

^c MEDICAL UNIVERSITY OF SOUTH CAROLINA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ACUTE PHASE PROTEIN; ALPHA2HS GLYCOPROTEIN; HYBRID PROTEIN; PLASMA PROTEIN;

ANIMAL; ARMYWORM; ARTICLE; BIOSYNTHESIS; CELL CULTURE; CELL LINE; HUMAN; ISOLATION AND PURIFICATION;

ACUTE-PHASE PROTEINS; ANIMALS; BLOOD PROTEINS; CELL LINE; HUMANS; RECOMBINANT FUSION PROTEINS; SPODOPTERA; TUMOR CELLS, CULTURED;

EID: 0032246232     PISSN: 12178950     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (27)

1. Triffitt, J.T., Gebauer, U., Asthon, B.A., Owen, M.E., Reynolds, J.J.: Origin of plasma α2HS -glycoprotein and its accumulation in bone. Nature 262, 226 (1976).
2. Gejyo, F., Chang, J.L., Bürgi, W., Schmid, K., Offner, G.D., Troxler, R.F., Van Halbeek, H., Dorland, L., Grewig, G.J., Vliegenthart, J.F.G.: Characterization of the B-chain of human plasma α2HS-glycoprotein. The complete amino acid sequence and primary structure of its heteroglycan. J Biol Chem 258, 4966 (1983).
3. Yoshioka, Y., Gejyo, F., Marti, T., Rickli, E.E., Bürgi, W., Offner, G.D., Troxler, R.F., Schmid, K.: The complete amino acid sequence of the A-chain of human plasma α2HS-glycoprotein. J Biol Chem 261, 1165 (1986).
4. Kellermann, J., Haupt, H., Auerswald, E.A., Müller-Esterl, W.: The arrangement of disulfide loops in human α2-HS glycoprotein. Similarity to the disulphide bridge structures of cystatins and kininogens. J Biol Chem 264, 14121 (1989).
5. Lewis, J.G., André, C.M.: Enhancement of human monocyte phagocytic function by α2HS glycoprotein. Immunology 42, 481 (1981).
6. Lewis, J.G., Crosier, P.S., André, C.M.: α2HS glycoprotein binds to lymphocytes transformed by Epstein-Barr virus. FEBS Lett 138, 37 (1982).
7. Jakab, L., Jakab, L., Kalabay, L., Pozsonyi, T., Cseh, K.: The effect of the α2-HS-glycoprotein on the mitogen induced lymphocyte blastic transformation and IL-2 production. Acta Physiol Hung 77, 25 (1991).
8. Lebreton, JP., Joisel, F., Raoult, J.P., Lannuzel, B., Rogez, J.P., Humbert, G.: Serum concentration of human alpha2 HS glycoprotein during the inflammatory process. J Clin Invest 64, 1118 (1979).
9. Dickson, I.R., Poole, A.R., Veis, A.: Localisation of plasma α2HS glycoprotein in mineralizing human bone. Nature 256, 430 (1975).
10. Dziegielewska, K.M., Brown, W.M., Casey, J.C., Christie, D.L., Foreman, R.C., Hill, R.M., Saunders, N.R.: The complete cDNA and amino acid sequence of bovine fetuin. Its homology with α2HS glycoprotein and relation to other members of the cystatin superfamily. J Biol Chem 256, 4354 (1990).
11. Haasemann, M., Nawratil, P., Müller-Esterl, W.: Rat tyrosine kinase inhibitor shows sequence similarity to human α2HS glycoprotein and bovine fetuin. Biochem J 274, 899 (1991).
12. Auberger, P., Falquerho, L., Contreres, J.O., Pages, G., Le Cam, G., Rossi, B., Le Cam, A.: Characterization of a natural inhibitor of the insulin receptor tyrosine kinase: cDNA, cloning, purification, and anti-mitogenic activity. Cell 58, 631 (1989).
13. Srinivas, P.R., Wagner, A.S., Reddy, L.V., Deutsch, D.D., Leon, M.A., Goustin, A.S., Grunberger, G.: Serum α2-HS-glycoprotein is an inhibitor of the human insulin receptor at the tyrosine kinase level. Mol Endocrinol 7, 1445 (1993).
14. Kalabay, L., Chavin, K., Lebreton, J.P., Robinson, K.A., Buse, M.G., Arnaud, P.: Human recombinant α2-HS glycoprotein is produced in insect cells as a full length inhibitor of the insulin receptor tyrosine kinase. Hormone Metab Res 30, 1 (1998).
15. Demetriou, M., Binkert, C., Sukhut, B., Tenenbaum, H.C., Dennis, J.W.: Fetuin/alpha2-HS glycoprotein is a transforming growth factor-beta type II receptor mimic and cytokine antagonist. J Biol Chem 271, 12755 (1996).
16. Ohnishi, T., Nakamura, O., Arakaki, N., Daikuhara, Y.: Effect of phosphorylated rat fetuin on the growth of hepatocytes in primary cultures in the presence of human hepatocyte-growth factor. Evidence that phosphorylated fetuin is a natural modulator of hepatocyte-growth factor. Eur J Biochem 243, 753 (1997).
17. Arnaud, P., Miribel, L., Emerson, D.L.: α2-HS glycoprotein. In: Di Sabato, G. (ed.): Immunochemical Techniques, PT M. Chemotaxis and Inflammation. Methods in Enzymology, Vol. 163. Academic Press, San Diego, USA, 1988. p. 431.
18. Kalabay, L., Mathur, S., Bobin, S., Arnaud, P.: Electrophoretic and isoelectric focusing analysis of human recombinant alpha2-HS glycoprotein produced in insect cells: Analysis of the post-translational events. Electrophoresis 17, 529 (1996).
19. Summers, M.D., Smith, G.E.: A Manual of Methods for Baculovirus Vectors and Insect Cell Culture Procedures. Tex Agric Exp Stn, Bull No. 1555, College Station, Texas, 1987.
20. Laemmli, U.K.: Cleavage of structural proteins during the assembly of the head of bacteriophage T1. Nature 227, 680 (1970).
21. Towbin, H., Staehelin, T., Gordon, J.: Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci 76, 4350 (1979).
22. Lebreton, J.P.: Purification of the human plasma alpha2-SH glycoprotein by zinc chelate affinity chromatography. FEBS Lett 80, 351 (1977).
23. Malone, J.D., Richards, M.: α2HS glycoprotein is chemotactic for mononuclear phagocytes. J Cell Physiol 132, 118 (1987).
24. Matsushima, K., Cheng, M., Migita, S.: Purification and physicochemical characterization of human α2HS-glycoprotein. Biochim Biophys Acta 701, 200 (1982).
25. van Oss, C.J., Bronson, P.M.: Isolation of human serum alpha-2 HS glycoprotein. Prep Biochem 4, 127 (1974).
26. O'Reilly, D.R., Miller, L.K., Luckow, V.A. (eds): Baculovirus Expression Vectors. A Laboratory Manual, Freeman W.H. and Co., New York, 1992.
27. Hayase, T., Rice, K.G., Dziegielewska, K.M., Kuhlenschmidt, M., Relly, T., Lee, Y.C.: Comparison of N-glycosides from fetuins from different species and human α2-HS-glycoprotein. Biochemistry 31, 4915 (1992).