Production of Bovine α-Lactalbumin in the Milk of Transgenic Pigs

Journal of Animal Science

Volumn 76, Issue 12, 1998, Pages 3072-3078

  Bleck, Gregory T ^a   White, Brett R ^a   Miller, David J ^a   Wheeler, Matthew B ^a,b  

^a UNIVERSITY OF ILLINOIS AT URBANA CHAMPAIGN   (United States)

^b NONE   (United States)

Author keywords

Lactose; Lactose synthase; Milk proteins

Indexed keywords

BOVINAE; SUIDAE; SUS SCROFA;

LACTALBUMIN;

ANIMAL; ANIMAL DISEASE; ARTICLE; BIOSYNTHESIS; CATTLE; CHEMISTRY; FEMALE; GENE EXPRESSION; GENETICS; METABOLISM; MILK; PHYSIOLOGY; POLYACRYLAMIDE GEL ELECTROPHORESIS; SWINE; TRANSGENIC ANIMAL; UDDER;

ANIMALS; ANIMALS, GENETICALLY MODIFIED; CATTLE; ELECTROPHORESIS, POLYACRYLAMIDE GEL; FEMALE; GENE EXPRESSION; LACTALBUMIN; MAMMARY GLANDS, ANIMAL; MILK; SWINE;

EID: 0032246125     PISSN: 00218812     EISSN: None     Source Type: Journal    
DOI: 10.2527/1998.76123072x     Document Type: Article

References (21)

1. Bleck, G. T., and R. D. Bromel. 1994. Variation in expression of a bovine α-lactalbumin transgene in milk of transgenic mice. J. Dairy Sci. 77:1897-1904.
2. Brew, K., and J. A. Grobler. 1992. α-Lactalbumin. In: P. F. Fox (Ed.) Advanced Dairy Chemistry, Proteins. Elsevier Sci. Publ. Ltd., New York.
3. Dobrinsky, J. R., L. A. Johnson, and D. Rath. 1996. Development of a culture medium (BECM-3) for porcine embryos: Effects of bovine serum albumin and fetal, bovine serum on embryo development. Biol. Reprod. 55:1069-1074.
4. Goodman, R. E., and F. L. Schanbacher. 1991. Bovine lactoferrin mRNA: Sequence, analysis and expression in the mammary gland. Biochem. Biophys. Res. Commun. 180:75-84.
5. Hartmann, P. E., I. McCauley, A. D. Gooneratne, and J. L. Whitely. 1984. Inadequacies of sow lactation: Survival of the fittest. In: Lactation Strategies, Symp. Zool. Soc. 51:301-326.
6. Hogan, B., F. Constantini, and E. Lacy. 1986. Manipulating the Mouse Embryo: A Laboratory Manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY.
7. Jeng, S. Y., G. T. Bleck, M. B. Wheeler, and R. Jiménez-Flores. 1997. Characterization and partial purification of bovine α-lactalbumin and β-casein produced in the milk of transgenic mice. J. Dairy Sci. 80:3167-3175.
8. Kim, H., and R. Jiménez-Flores. 1993. Two-dimensional analysis of skim milk proteins using preparative isoelectric focusing followed by polyacrylamide gel electrophoresis. J. Food Biochem. 16:307-321.
9. Klobasa, F., E. Werhahn, and J. E. Butler. 1987. Composition of sow milk during lactation. J. Anim. Sci. 64:1458-1466.
10. Kuhn, N. J., D. T. Carrick, and C. J. Wilde. 1980. Lactose synthesis: Possibilities of regulation. J. Dairy Sci. 63:328-336.
11. Laemmli, U. K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature (Lond.) 227: 680-685.
12. Larson, B. L. 1985. Lactation. Iowa State University Press, Ames.
13. Larson, B. L. 1985. Lactation. Iowa State University Press, Ames. Mao, F. C., R. D. Bremel, and M. R. Dentine. 1991. Serum concentrations of the milk proteins α-lactalbumin and β-lactoglobulin in pregnancy and lactation: correlations with milk and fat yields in dairy cattle. J. Dairy Sci. 74:2952-2958.
14. McKenzie, H. A., and F. H. White. 1991. Lysozyme and α-lactalbumin: structure, function, and interrelationships. Adv. Protein Chem. 41:173-315.
15. ® User's Guide (Release 6.03). SAS Inst. Inc., Cary, NC.
16. Shamay, A., S. Solinas, V. G. Pursel, R. A. McKnight, L. Alexander, C. Beattie, L. Hennighausen, and R. J. Wall. 1991. Production of the mouse whey acidic protein in transgenic pigs during lactation. J. Anim. Sci. 69:4552-4562.
17. Stacey, A., A. Schnieke, M. Kerr, A. Scott, C. McKee, I. Cottingham, B. Binas, C. Widle, and A. Coleman. 1995. Lactation is disrupted by α-lactalbumin deficiency and can be restored by human α-lactalbumin gene replacement in mice. Proc. Natl. Acad. Sci. USA 92:2835-2839.
18. Stinnakre, M. G., J. L. Vilotte, S. Soulier, and J.-C. Mercier. 1994. Creation and phenotypic analysis of α-lactalbumin-deficient mice. Proc. Natl. Acad. Sci. USA 91:6544-6548.
19. Teles, F. F., C. K. Young, and J. W. Stull. 1978. A method for rapid determination of lactose. J. Dairy Sci. 61:506-508.
20. Wall, R. J., V. G. Pursel, A. Shamay, R. A. McKnight, C. W. Pittius, and L. Hennighausen. 1991. High-level synthesis of a heterologous milk protein in the mammary glands of transgenic swine. Proc. Natl. Acad. Sci. USA 88:1696-1700.
21. Zou, S., D. G. McLaren, and W. L. Hurley. 1992. Pig colostrum and milk composition: comparisons between Chinese Meishan and US breeds. Livest. Prod. Sci. 30:115-127.