A sensitive fluorometric assay for determining hydrogen peroxide-mediated sublethal and lethal endothelial cell injury

Endothelium: Journal of Endothelial Cell Research

Volumn 6, Issue 2, 1998, Pages 143-151

  Oral, Haluk B ^a,b,c   George, Andrew J T ^a   Haskard, Dorian O ^a,b  

^a HAMMERSMITH HOSPITAL   (United Kingdom)

^b NATIONAL HEART AND LUNG INSTITUTE   (United Kingdom)

^c ULUDAG UNIVERSITY   (Turkey)

Author keywords

Calcein AM; Cell injury; Endothelial cell; Hydrogen peroxide

Indexed keywords

CALCEIN AM; FLUORESCEIN DERIVATIVE; FLUORESCENT DYE; HYDROGEN PEROXIDE; OXIDIZING AGENT;

ARTICLE; CELL LINE; CYTOLOGY; FLUOROMETRY; HUMAN; METABOLISM; METHODOLOGY; SENSITIVITY AND SPECIFICITY; VASCULAR ENDOTHELIUM;

CELL LINE; ENDOTHELIUM, VASCULAR; FLUORESCEINS; FLUORESCENT DYES; FLUOROMETRY; HUMANS; HYDROGEN PEROXIDE; OXIDANTS; SENSITIVITY AND SPECIFICITY;

EID: 0032233776     PISSN: 10623329     EISSN: None     Source Type: Journal    
DOI: 10.3109/10623329809072201     Document Type: Article

References (34)

1. Blum J. and Fridovich I. (1985) Inactivation of glutathione peroxidase by superoxide radical. Archives of Biochemistry and Biophysics, 240, 500-508.
2. Bozyczko-Coyne D., McKenna B.W., Connors T.J. and Neff N.T. (1993). A rapid fluorometric assay to measure neuronal survival in vitro. Journal of Neuroscience Methods, 50, 205-216.
3. Bradley J.R., Johnson D.R. and Pober J.S. (1993) Endothelial activation by hydrogen peroxide: selective increases of intracellular adhesion molecule-1 and major histocompatibility complex Class I. American Journal of Pathology, 142, 1598-1609.
4. Brigham K.L., Meyrick B., Berry L.C. and Repine J.E. (1987) Antioxidants protect cultured bovine lung endothelial cells from injury by endotoxin. Journal of Applied Physiology, 63, 840-850.
5. Bruning J.W., Kardol M.J. and Arentzen R. (1980) Carboxyfluorescein fluorochromasia assays. I. Non-radioactively labeled cell mediated lympholysis. Journal of Immunological Methods, 33, 33-44.
6. Brunner K.T., Mauel J., Cerotinni J.C. and Chapuis B. (1968) Quantitative assay of the lytic action of immune lymphoid cells on 51-Cr-labelled allogeneic target cells in vitro; inhibition by isoantibody and by drugs. Immunology, 14, 181-196.
7. Collins T., Krensky A.M., Clayberger C., Fiers W., Gimbrone M.A., Burakoff S.J. and Pober J.S. (1984) Human cytolytic T lymphocyte interactions with vascular endothelium and fibroblasts: Role of effector and target cell molecules. Journal of Immunology, 133, 1878-1884.
8. DeClerk L.S., Bridts C.H., Mertens A.M., Moens M.M. and Stevens W.J. (1993) Use of fluorescent dyes in the determination of adherence of human leucocytes to endothelial cells and effect of the fluorochromes on cellular functions. Journal of Immunological Methods, 172, 115-124.
9. Deisseroth A. and Dounce A.L. (1970) Catalase: Physical and chemical properties, mechanisms of catalysis, and physiological role. Physiological Reviews, 50, 319-375.
10. Edgell C-J.S., McDonald C.C. and Graham J.B. (1983) Permanent cell line expressing human factor VIII-related antigen established by hybridization. Proceedings of the National Academy of Sciences USA, 80, 3734-3737.
11. Fantone J.C. and Ward P.A. (1982) Role of oxygen-derived free radicals and metabolites in leucocyte-dependent inflammatory reactions. American Journal of Pathology, 107, 395-418.
12. Getzoff E.D., Tainer J.A., Weiner P.K., Kollmann P.A., Richardson J.S. and Richardson J.C. (1983) Electrostatic recognation beween superoxide and copper, zinc superoxide dismutase. Nature, 306, 287-290.
13. Harlan J.M., Killen P.D., Harker L.A., Striker G.E. and Wright D.G. (1981) Neutrophil-mediated endothelial injury: Mechanisms of cell detachment. Journal of Clinical Investigation, 68, 1394-1403.
14. Haugland R.P. (1996) Fluorescent dyes for assessing vital cell functions. In: Handbook of Fluorescent Probes and Research Chemicals (ed. K.D. Larison), Molecular Probes, Eugene, OR.
15. Jaffe E.A., Nachman N.L., Becker C.G. and Minick C.R. (1973) Culture of human endothelial cells derived from umbilical veins. Journal of Clinical Investigation, 52, 2745-2756.
16. Kolber M.A., Quinones R.R., Gress R.E. and Henkart P.A. (1988) Measurement of cytotoxicity by target cell release and retention of the fluorescent dye bis-carboxyethyl-carboxyfluorescein (BCECF). Journal of Immunological Methods, 108, 255-264.
17. Korzeniewski C. and Callewaert D.M. (1983) An anzyme release assay for natural cytotoxicity. Journal of Immunological Methods, 64, 313-320.
18. Lewis M.S., Whatley R.E., Cain P., McIntyre T.M., Prescott S.M. and Zimmerman G.A. (1988) Hydrogen peroxide stimulates the synthesis of platelet-activating factor by endothelium and induces endothelial cell-dependent neutrophil adhesion. Journal of Clinical Investigation, 82, 2045-2055.
19. Lo S.K., Janakidevi K., Lai L. and Malik A.B. (1993) Hydrogen peroxide-induced increase in endothelial adhesiveness is dependent on ICAM-1 activation. American Journal of Physiology, 264, L406-L412.
20. MacCoubrey I.C., Moore P.L. and Haugland R.P. (1990) Quantitative fluorescence measurement of cell viability (cytotoxicity) with a multi-well plate scanner. Journal of Cell Biology, 111, 58a (303).
21. McCay P.B. (1985). Vitamin E: Interactions with free radicals and ascorbate. Annual Review of Nutrition., 5, 323-340.
22. Moore P.L., MacCourbey I.C. and Haugland R.P. (1990) A rapid, pH sensitive, two color fluorescent viability (cytotoxicity) assay. Journal of Cell Biology, 111, 58a (304).
23. Natarajan V., Taher M.M., Roehm B., Perinandi N.L., Schmid H.H.O., Kiss Z. and Garcia J.G.N. (1993) Activation of endothelial cell phospholipase D by hydrogen peroxide and fatty acid hydroperoxidase. Journal of Biological Chemistry, 268, 930-937.
24. Patel K.D., Zimmerman G.A., Prescott S.M., McEver R.P. and McIntyre T.M. (1991) Oxygen radicals induce human endothelil cells to express GMP-140 and bind neutrophils. Journal of Cell Biology, 112, 749-759.
25. Searle A.J. and Willson R.L. (1980). Glutathione peroxidase: effect of superoxide, hydroxyl and bromine free radicals on enzyme activity. International Journal of Radiation Biology., 37, 213-217.
26. Shatos M.A., Doherty J.M. and Hoak J.C. (1991) Alterations in human vascular endothelial cell function by oxygen free radicals; platelet adherence and prostacyclin release. Arteriosclerosis Thromb., 11, 594-601.
27. Shatos M.A., Doherty J.M., Orfeo T, Hoak J.C., Collen D. and Stump D.C. (1992) Modulation of fibrinolytic response to cultured human vascular endothelium by extracellularly generated oxygen radicals. Journal of Biological Chemistry., 267, 597-601.
28. Siflinger-Birnboim, A., Goligorsky, M.S., Del Vecchio, P.J. and Malik, A..B. (1992) Activation of protein kinase C pathway contributes to hydrogen peroxide-induced increase in endothelial permeability. Laboratory Investigation, 67, 24-30.
29. Taher M.M., Garcia J.G.N. and Natarajan V. (1993) Hydroperoxide-induced diacylglycerol formation and protein kinase C activation in vascular endothelial cells. Archives of Biochemistryand Biophysics, 303, 260-266.
30. Thomhill M.H., Li J. and Haskard D.O. (1993) Leucocyte endothelial cell adhesion: a study human umbilical vein endothelial cells and the endothelial cell Line EA-hy-926. Scandinavian Journal of Immunology, 38, 279-286.
31. Varani J, Ginsburg I, Schuger L., Gibbs D.F., Bromberg J., Johnson, K.J. Ryan U.S. and Ward P.A. (1989) Endothelial cell killing by neutrophils: synergistic interaction of oxygen products and proteases. American Journal of Pathology, 135, 435-438.
32. Wellicome S.M., Thomhill M.H., Pitzalis C., Thomas D.S., Lanchburry J.S.S., Panayi, G.S. and Haskard D.O. (1990). A monoclonal antibody that detects a novel antigen to endothelial cells that is induced by tumor necrosis factor, IL-1, or lipopolysaccharide. Journal of Immunology, 144, 2558-2565.
33. Weston S.A. and Parish C.R. (1990) New florescent dyes for lymphocyte migration studies. Analysis of flow cytometry and fluorescence microscopy. Journal of Immunological Methods, 133, 87-97.
34. Wilson J., Winter M. and Shasby D.M. (1990) Oxidants, ATP depletion, and endothelial permeability to macromolecules. Blood, 12, 2578-2582.