Pulse width considerations for multiple-photon excitation laser scanning fluorescence imaging

Proceedings of SPIE - The International Society for Optical Engineering

Volumn 3260, Issue , 1998, Pages 115-122

  Wokosin, D L ^a   White, J G ^a   Dymott, Mike ^a  

^a UNIVERSITY OF WISCONSIN MADISON   (United States)

Author keywords

Laser scanning fluorescence microscopy; Phototoxicity; Three photon excitation; Two photon excitation; Ultrafast pulsed lasers; UV irradiation

Indexed keywords

FLUORESCENCE; IMAGING TECHNIQUES; IRRADIATION; LASER DAMAGE; LASER MODE LOCKING; MULTIPHOTON PROCESSES; PULSED LASER APPLICATIONS; SAPPHIRE; TOXICITY; ULTRASHORT PULSES;

LASER SCANNING FLUORESCENCE MICROSCOPY;

OPTICAL ENGINEERING;

EID: 0032226162     PISSN: 0277786X     EISSN: None     Source Type: Conference Proceeding    
DOI: 10.1117/12.307082     Document Type: Conference Paper

References (9)

1. Multiphoton fluorescence excitation: New spectral windows for biological nonlinear microscopy
2. Three-photon exctation fluorescence imaging of biological specimens using an all-solid-state laser
3. All-solid-state ultra-fast lasers facilitate multi-photon excitation fluorescence imaging
4. Construction of a two-photon microscope and optimisation of illumination pulse duration
5. Measurement of group delay dispersion of high numerical aperture objective lenses using two-photon excited fluorescence
6. Compression of optical pulses chirped by self-phase modulation in fibers
7. Measuring seratonin distribution in live cells with three-photon excitation
8. Multi-photon excitation imaging with an all-solid-state laser
9. Optimization of the design of a multi-photon excitation laser scanning fluorescence imaging system