Detection of trypanosome DNA in serologically positive but aparasitaemic horses suspected of dourine in Ethiopia

Tokai Journal of Experimental and Clinical Medicine

Volumn 23, Issue 6, 1998, Pages 303-308

  Clausen, Peter Henning ^a,d   Gebreselassie, Gebrehiwot ^a   Abditcho, Sintayehu ^b   Mehlitz, Dieter ^a   Staak, Christian ^c  

^a FREIE UNIVERSITÄT BERLIN   (Germany)

^b Natl Animal Disease Research Centre   (Ethiopia)

^c Fed Inst Hlth Protect C   (Germany)

^d Inst Parasitol and Trop Vet Med   (Germany)

Author keywords

Diagnosis; Dourine; Ethiopia; PCR; Trypanosoma equiperdum

Indexed keywords

PROTOZOAL DNA; PROTOZOON ANTIBODY;

ANIMAL; ANIMAL PARASITOSIS; ARTICLE; BLOOD; ETHIOPIA; FEMALE; HORSE; HORSE DISEASE; ISOLATION AND PURIFICATION; MALE; PARASITOLOGY; POLYMERASE CHAIN REACTION; SEROLOGY; TRYPANOSOMA;

ANIMALS; ANTIBODIES, PROTOZOAN; DNA, PROTOZOAN; DOURINE; ETHIOPIA; FEMALE; HORSE DISEASES; HORSES; MALE; POLYMERASE CHAIN REACTION; SEROLOGIC TESTS; TRYPANOSOMA;

EID: 0032222842     PISSN: 03850005     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (17)

1. Alemu T, Luckins AG, Phipps LP. Reid SWJ and Holmes PH: The use of enzyme linked immunosorbent assays to investigate the prevalence of Trypanosoma equiperdum in Ethiopian horses. Vet Parasitol 71: 239-250, 1997.
2. Alton GG, Jones LM, Angus RD, Verger JM: Techniques for the brucellosis laboratory. INRA, Paris, 1988.
3. Artama WT, Agey MW and Donelson, JE: DNA comparisons of Trypanosoma evansi (Indonesia) and Trypanosoma brucei spp. Parasitology 104: 67-74, 1992.
4. Barrowman PR: Observations on the transmission, immunology, clinical signs and chemotherapy of dourine (Trypanosoma equiperdum infection) of horses, with special reference to cerebrospinal fluid. Onderstepoort J vet Res 43: 55-66, 1976.
5. Clausen P-H, Waiswa C, Moritz A, Steuber S: Post treatment detection of cryptic/latent parasitic infections (bovine trypanosoinosis, canine leishmaniosis) by DNA amplification. In: Hygiene und Mikrobiologie. Methodische Entwicklungen in der Mikrobiologischen Nukleinsäure-Diagnostik. Abstract-Band. Gobel U (Ed), Einhorn-Presse Verlag, Reinbek, 24, 1997.
6. Clausen P-H, Wiemann A, Patzelt R, Kakaire D, Poetzsch C, Peregrine A, and Mehlitz D: Use of a PCR assay for the specific and sensitive detection of Trypanosoma spp. in naturally infected dairy cattle in peri-urban Kampala, Uganda. Ann NY Acad Sci 849: 21-31, 1998.
7. Dagnachew Z, Shafo K and Kelil A: An investigation of dourine in Arsi administrative region. Ethiop Med J 4: 3-19, 1980.
8. Hoare CA: The Trypanosomes of Mammals. Trypanosoma (Trypanozoon) equiperdum Doflein, 1901. Blackwell Sci. Publ., Oxford and Edinburgh: 593-604, 1972.
9. Kaminsky R, Chuma F and Zweygarth E: Trypanosoma brucei brucei : Expression of drug resistance in vitro. Exp Parasitol 69: 281-289, 1989.
10. Kanmogne GD, Asonganyi T, and Gibson WC: Detection of Trypanosoma brucei gambiense, in serologically positive but aparasitaemic sleeping-sickness suspects in Cameroon, by PCR. Ann Trop Med Parasitol 90: 475 483, 1996.
11. Lanham SM and Godfrey DG: Isolation of salivarian trypanosomes from man and other mamals using DEAE-cellulose. Exp Parasitol 28: 521-584, 1970.
12. Masake RA, Majiwa PAO, Moloo SK, Makau JM, Njuguna JT, Maina M, Kabata J, Ole-Moi Yoi OK and Nantulya VM: Sensitive and specific detection of Trypanosoma vivax using the polymerase chain reaction. Exp Parasitol 85: 193-205, 1997.
13. O.I.E.: Manual of Standards for Diagnostic Tests and Vaccines. Dourine. Office International des Epizooties: 394-399, 1996.
14. Schares G and Mehlitz D: Sleeping sickness in Zaire: a nested polymerase chain reaction improves the identification of Trypanosoma (Trypanozoon) brucei gambiense by specific kinetoplast DNA probes. Trop Med Int Health 1: 59-70, 1996.
15. Staak C, Clausen P-H, Steuber S, Thiele H and Ortmann G: Serologischer und klinischer Beschälseuche-Befund bei einem Hengst. Amtstierärztl. Dienst und Lebensmittelkontrolle 3: 248-249, 1996.
16. Woo PTK: The haematocrit centrifuge technique for the diagnosis of African trypanosomiasis. Acta Trop 27: 384-386, 1970.
17. Wuyts N, Chokesajjawatee N and Panyim S: A simplified and highly sensitive detection of Trypanosoma evansi by DNA amplification. South-East Asian J Trop Med Public Health 25: 266-271, 1994.