Transformation of Fusarium culmorum with the β-D-glucuronidase (GUS) reporter gene: A system for studying host-pathogen relationships and disease control

Physiological and Molecular Plant Pathology

Volumn 53, Issue 5-6, 1998, Pages 253-268

  Doohan, F M ^a   Smith, P ^a   Parry, D W ^b   Nicholson, P ^a  

^a NORWICH RESEARCH PARK   (United Kingdom)

^b HORTICULTURE RESEARCH INTERNATIONAL   (United Kingdom)

Author keywords

Fungicides; Fusarium culmorum; Transformation; D glucuronidase gene

Indexed keywords

BETA GLUCURONIDASE; ENZYME ACTIVITY; FUNGAL GROWTH; FUNGICIDE TOLERANCE; GENETIC TRANSFORMATION; HOST PATHOGEN INTERACTION; HYGROMYCIN PHOSPHOTRANSFERASE; MICROSCOPY; MITOSIS; PATHOGENICITY; PLANT DISEASE CONTROL; PLASMID; REPORTER GENE; SCREENING; SOUTHERN BLOTTING; WHEAT;

ESCHERICHIA COLI; FUNGI; FUSARIUM CULMORUM; PROKARYOTA; TRITICUM AESTIVUM;

EID: 0032216203     PISSN: 08855765     EISSN: None     Source Type: Journal    
DOI: 10.1006/pmpp.1998.0178     Document Type: Article

References (34)

1. 1. Ashby, AM, Johnston K. 1993. Expression of the E. coli β-glucuronidase gene in the light leaf spot pathogen Pyrenopeziza brassicae and its use as a reporter gene to study the developmental interaction in fungi. Mycological Research 97: 575-581.
2. 2. Bunkers GJ. 1991. Expression of Escherichia coli β-glucuronidase gene in Pseudocercosporella herpotrichoides. Applied and Environmental Microbiology 57: 2896-2900.
3. 3. Couteaudier Y, Daboussi MJ, Eparvier A, Langin T, Orcival J. 1993. The GUS gene fusion system (Escherichia coli β-D-glucuronidase gene), a useful tool in studies of root colonisation by Fusarium oxysporum. Applied and Environmental Microbiology 59: 1767-1773.
4. 4. Cooley RN, Shaw RK, Franklin FCH, Caten CE. 1988. Transformation of the phytopathogenic fungus Septoria nodorum to hygromycin B resistance. Current Genetics 13: 383-389.
5. 5. Curragh HJ, Mooibroek H, Wessels JGH, Marcant R, Mullan E. 1992. Protoplast formation and DNA-mediated transformation of Fusarium culmorum to hygromycin B resistance. Mycological Research 97: 313-317.
6. 6. Doohan FM. 1998. Molecular techniques for studying Fusarium ear blight of wheat and its control. PhD. Thesis, Open University, Buckingham, UK.
7. 7. Doohan FM, Jenkinson P, Parry DW, Nicholson P. 1998. The use of species-specific PCR-based assays to analyze fusarium ear blight of wheat. Plant Pathology 47: 197-205.
8. 8. Don RH, Cox PT, Wainwright BJ, Baker K, Mattick JS. 1991. "Touchdown" PCR to circumvent spurious priming during gene amplification. Nucleic Acids Research 19: 4008.
9. 9. Gould JH, Smith RH. 1989. A non-destructive assay for GUS in the media of plant tissue cultures. Plant Molecular Biology Reporter 7: 209-216.
10. 10. Gritz L, Davies J. 1983. Plasmid-encoded hygromycin B resistance: the sequence of hygromycin B phosphotransferase gene and its expression in Escherichia coli and Saccharomyces cerevisiae. Gene 25: 179-188.
11. 11. Hohn TM, Desjardins AE. 1992. Isolation and gene disruption of the Tox5 gene encoding trichodiene synthase in Gibberella pulicaris. Molecular Plant - Microbe Interactions 5: 249-256.
12. 12. Jefferson RA, Burgess SM, Hirsh D. 1986. β-Glucuronidase from Escherichia coli as a gene-fusion marker. Proceedings of the National Academy of Sciences USA 83: 8447-8451.
13. 13. Jefferson RA, Kavanagh AA & Bevan MW. 1987. GUS fusions: β-glucuronidase as a sensitive and versatile gene fusion marker in higher plants. European Molecular Biology Organisation Journal 6: 3901-3907.
14. 14. Jefferson RA, Klass M, Wolf N, Hirsh D. 1987. Expression of chimeric genes in Caenorhabditis elegans. Journal of Molecular Biology 193: 41-46.
15. 15. Jefferson RA. 1989. The GUS reporter gene system. Nature 342: 837-838.
16. 16. Kistler HC, Benny V. 1992. Autonomously replicating plasmids and chromosome rearrangement during transformation of Nectria haematococca. Gene 117: 81-89.
17. 17. Lees A. 1995. Diagnosis and control of foot rot pathogens of wheat. Ph.D. Thesis, Open University, Buckingham, UK.
18. 18. Mönke E, Schäfer W. 1993. Transient and stable gene expression in the fungal maize pathogen. Cochliobolus heterostrophus after transformation with the β-glucuronidase gene. Molecular and General Genetics 241: 73-80.
19. 19. Nelson PE, Toussoun TA, Marasas WFO. 1983. Fusarium species, an illustrated manual for identification. University Park, U.S.A.: The Pennsylvania State University Press.
20. 20. Nicholson P, Lees AK, Maurin N, Parry DW, Pezanoor HN. 1996. Development of a PCR assay to identify and quantify Microdochium nivale var. nivale and Microdochium nivale var. majus in wheat. Physiological and Molecular Plant Pathology 48: 257-271.
21. 21. Nirenberg H. 1976. Unterschung über die morphologische und biologische differenzierung in der Fusarium-section liseola. Mitteilungen aus der Biologische Bundesanstalt für Land-und Fortwirtschaft, Berlin-Dahlem 169: 1-117.
22. 22. Oliver RP, Farman ML, Jones JDG, Hammond-Kossack KE. 1993. Use of fungal transformants expressing β-glucuronidase activity to detect infection and measure hyphal biomass in infected plant tissues. Molecular Plant-Microbe Interactions 6: 521-525.
23. 23. Parry DW. 1991. Fusarium diseases of winter wheat. In: Schering Agrifile Issue 3. Saffron Walden, U.K.: Schering Agrochemicals Publications, pp. 6-14.
24. 24. Parry DW, Pettitt TR, Jenkinson P, Lees AK. 1994. The cereal Fusarium complex. In: Blakeman, P. & Williamson, B., eds. Ecology of Plant Pathogens. Wallingford, U.K.: CAB International, pp. 301-320.
25. 25. Pomeranz Y, Bechter DB, Sauer DB, Seitz LM. 1990. Fusarium head blight (scab) in cereal grains. In: Pomeranz Y, ed. Advances in Cereal Science and Technology. American Association of Cereal Chemists, pp. 373-433.
26. 26. Roberts IN, Oliver RP, Punt PJ, van den Hondel CAMJJ. 1989. Expression of the E. coli β-glucuronidase gene in industrial and phytopathogenic filamentous fungi. Current Genetics 15: 177-180.
27. 27. Salch YP, Beremand MN. 1993. Gibberella pulicaris transformants: state of transforming DNA during asexual and sexual growth. Current Genetics 23: 343-350.
28. 28. Saur L. 1991. Sources of resistance to head blight caused by Fusarium culmorum in bread wheat and related species. Agronomie 11: 535-541.
29. 29. Schilling AG, Möller EM, Geiger HG. 1996. Polymerase chain reaction-based assays for species-specific detection of Fusarium culmorum, F. graminearum and F. avenaceum. Phytopathology 86: 515-22.
30. 30. Sivan A, Stasz TE, Hemmat M, Hayes CK, Harman GE. 1992. Transformation of Trichoderma spp. with plasmids conferring hygromycin B resistance. Mycologia 84: 687-694.
31. 31. Snedecor GW, Cochran WG. 1980. Statistical Methods. Iowa, U.S.A.: The Iowa State University Press.
32. 32. Snijders CHA, Perkowski J. 1990. Effects of head blight caused by Fusarium culmorum on toxin content and weight of wheat kernels. Phytopathology 80: 566-570.
33. 33. Thrane C, Lübeck M, Green H, Degefu Y, Allerup S, Thrane U, Funck Jensen D. 1995. A tool for monitoring Trichoderma harzianum: I. Transformation with the GUS gene by protoplast technology. Phytopathology 85: 1428-1435.
34. 34. Turgeon BG, Garber RC, Yoder OC. 1985. Transformation of the fungal maize pathogen Cochliobolus heterotrophus using the Aspergillus nidulans amdS gene. Molecular and General Genetics 201: 450-453.