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Volumn 23, Issue 6, 1998, Pages 350-359

The use of capillary viscometry, reducing end-group analysis, and size exclusion chromatography combined with multi-angle laser light scattering to characterize endo-1,4-β-d-glucanases on carboxymethylcellulose: A comparative evaluation of the three methods

Author keywords

Carboxymethylcellulose; Disodium 2,2' bicinchoninate; Endo 1,4 D glucanase; Enzymatic hydrolysis; Multi angle laser light scattering; Size exclusion chromatography; Viscometry

Indexed keywords

CATALYST ACTIVITY; CATALYST SELECTIVITY; CELLULOSE DERIVATIVES; CHEMICAL BONDS; HYDROLYSIS; LIGHT SCATTERING; MOLECULAR WEIGHT; SIZE EXCLUSION CHROMATOGRAPHY; SODIUM COMPOUNDS; VISCOSITY MEASUREMENT;

EID: 0032212169     PISSN: 01410229     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0141-0229(98)00052-0     Document Type: Article
Times cited : (32)

References (40)
  • 1
    • 0025605767 scopus 로고
    • Trends in biochemistry and enzymology of cellulose degradation
    • Klyosov A.A. Trends in biochemistry and enzymology of cellulose degradation. Biochemistry. 29:(47):1990;10577-10585.
    • (1990) Biochemistry , vol.29 , Issue.47 , pp. 10577-10585
    • Klyosov, A.A.1
  • 2
    • 0026586210 scopus 로고
    • Fungal cellulases
    • Wood T.M. Fungal cellulases. Biochem. Soc. Trans. 20:(1):1992;46-53.
    • (1992) Biochem. Soc. Trans. , vol.20 , Issue.1 , pp. 46-53
    • Wood, T.M.1
  • 4
    • 0022229142 scopus 로고
    • Measurement of saccharification by cellulases
    • Mullings R. Measurement of saccharification by cellulases. Enzyme Microb. Technol. 7:1985;586-591.
    • (1985) Enzyme Microb. Technol. , vol.7 , pp. 586-591
    • Mullings, R.1
  • 6
    • 0000807891 scopus 로고
    • A comparative investigation of various cellulase assay procedures
    • Canevascini G., Gattlen C. A comparative investigation of various cellulase assay procedures. Biotechnol. Bioeng. XXIII:1981;1573-1590.
    • (1981) Biotechnol. Bioeng. , vol.23 , pp. 1573-1590
    • Canevascini, G.1    Gattlen, C.2
  • 7
    • 0000500353 scopus 로고
    • Viscometric and colorimetric studies on the hydrolysis of carboxymethylcellulose by cellulase enzymes
    • Esterbauer H., Schurz J., Wirtl A. Viscometric and colorimetric studies on the hydrolysis of carboxymethylcellulose by cellulase enzymes. Cellulose Chem. Technol. 19:1985;341-355.
    • (1985) Cellulose Chem. Technol. , vol.19 , pp. 341-355
    • Esterbauer, H.1    Schurz, J.2    Wirtl, A.3
  • 8
    • 0014200134 scopus 로고
    • Enzymic degradation of polymers. I. Viscometric method for the determination of enzymic activity
    • Almin K.E., Eriksson K.E. Enzymic degradation of polymers. I. Viscometric method for the determination of enzymic activity. Biochim. Biophys. Acta. 139:(2):1967;238-247.
    • (1967) Biochim. Biophys. Acta , vol.139 , Issue.2 , pp. 238-247
    • Almin, K.E.1    Eriksson, K.E.2
  • 9
    • 0019628039 scopus 로고
    • Improved viscometric assay for cellulase
    • Manning K. Improved viscometric assay for cellulase. J. Biochem. Biophys. Methods. 5:1981;189-202.
    • (1981) J. Biochem. Biophys. Methods , vol.5 , pp. 189-202
    • Manning, K.1
  • 10
    • 0042687350 scopus 로고
    • Viscometric determination of molecular weight
    • A.R. Cooper. John Wiley & Sons
    • Kamide K., Saito M. Viscometric determination of molecular weight. Cooper A.R. Determination of Molecular Weight. 1989;145-199 John Wiley & Sons.
    • (1989) Determination of Molecular Weight , pp. 145-199
    • Kamide, K.1    Saito, M.2
  • 11
    • 0017802798 scopus 로고
    • Characterization of endo-1,4-β-D-glucanases purified from Trichoderma viride
    • Shoemaker S.P., Brown R.D. Jr. Characterization of endo-1,4-β-D-glucanases purified from Trichoderma viride. Biochim. Biophys. Acta. 523:1978;147-161.
    • (1978) Biochim. Biophys. Acta , vol.523 , pp. 147-161
    • Shoemaker, S.P.1    Brown R.D., Jr.2
  • 12
    • 0015150662 scopus 로고
    • Viscometric determination of carboxymethylcellulase in standard international units
    • Hulme M.A. Viscometric determination of carboxymethylcellulase in standard international units. Arch. Biochem. Biophys. 147:(1):1971;49-54.
    • (1971) Arch. Biochem. Biophys. , vol.147 , Issue.1 , pp. 49-54
    • Hulme, M.A.1
  • 13
    • 9044242316 scopus 로고
    • Viscometric determination of carboxymethylcellulase activity
    • W.A. Wood, Kellogg S.T. NY: Academic Press
    • Hulme M.A. Viscometric determination of carboxymethylcellulase activity. Wood W.A., Kellogg S.T. Methods in Enzymology. Vol. 160:1988;130-135 Academic Press, NY.
    • (1988) Methods in Enzymology , vol.160 , pp. 130-135
    • Hulme, M.A.1
  • 14
    • 0001075923 scopus 로고
    • Kinetics of the action of cellulolytic enzymes from Geotrichum candidum. Viscometric analysis of the kinetics of the hydrolysis of carboxymethylcellulose
    • Rabinovich M.L., Klesov A.A., Berezin I.V. Kinetics of the action of cellulolytic enzymes from Geotrichum candidum. Viscometric analysis of the kinetics of the hydrolysis of carboxymethylcellulose. Bioorg. Khim. 3:1977;405-414.
    • (1977) Bioorg. Khim. , vol.3 , pp. 405-414
    • Rabinovich, M.L.1    Klesov, A.A.2    Berezin, I.V.3
  • 15
    • 0016638184 scopus 로고
    • Enzymatic studies on a cellulase system of Trichoderma viride. II. Purification and properties of two cellulases
    • Okada G. Enzymatic studies on a cellulase system of Trichoderma viride. II. Purification and properties of two cellulases. J. Biochem. 77:1975;33-42.
    • (1975) J. Biochem. , vol.77 , pp. 33-42
    • Okada, G.1
  • 16
    • 0017799256 scopus 로고
    • Enzymic activities of endo-1,4-β-D-glucanases purified from Trichoderma viride
    • Shoemaker S.P., Brown R.D. Jr. Enzymic activities of endo-1,4-β-D-glucanases purified from Trichoderma viride. Biochim. Biophys. Acta. 523:1978;133-146.
    • (1978) Biochim. Biophys. Acta , vol.523 , pp. 133-146
    • Shoemaker, S.P.1    Brown R.D., Jr.2
  • 17
    • 0344761502 scopus 로고
    • Enzymatic hydrolysis of cellulose. I-Activity and component composition of the cellulase complexes from various sources
    • Klesov A.A., Rabinovich M.L., Sinitsyn A.P., Churilova I.V., Grigorash S.Y. Enzymatic hydrolysis of cellulose. I-Activity and component composition of the cellulase complexes from various sources. Sov. J. Bioorg. Chem. 6:1981;662-667.
    • (1981) Sov. J. Bioorg. Chem. , vol.6 , pp. 662-667
    • Klesov, A.A.1    Rabinovich, M.L.2    Sinitsyn, A.P.3    Churilova, I.V.4    Grigorash, S.Y.5
  • 18
    • 0022425315 scopus 로고
    • The cellulase of Trichoderma viride; Purification, characterization, and comparison of all detectable endoglucanases, exoglucanases, and β-glucosidases
    • Beldman G., Searle-van Leeuwen M.F., Rombout F.M., Voragen F.G.J. The cellulase of Trichoderma viride; purification, characterization, and comparison of all detectable endoglucanases, exoglucanases, and β-glucosidases. Eur. J. Biochem. 146:1985;301-308.
    • (1985) Eur. J. Biochem. , vol.146 , pp. 301-308
    • Beldman, G.1    Searle-Van Leeuwen, M.F.2    Rombout, F.M.3    Voragen, F.G.J.4
  • 19
    • 33750423631 scopus 로고
    • Notes on sugar determination
    • Somogyi M.J. Notes on sugar determination. J. Biol. Chem. 195:1952;19-23.
    • (1952) J. Biol. Chem. , vol.195 , pp. 19-23
    • Somogyi, M.J.1
  • 20
    • 33747333106 scopus 로고
    • Use of dinitrosalicylic acid reagent for determination of reducing sugar
    • Miller G.L. Use of dinitrosalicylic acid reagent for determination of reducing sugar. Anal. Chem. 31:1959;426-428.
    • (1959) Anal. Chem. , vol.31 , pp. 426-428
    • Miller, G.L.1
  • 22
    • 0027651651 scopus 로고
    • Activity studies of eight purified cellulases: Specificity, synergism, and binding domain effects
    • Irwin D.C., Spezio M., Walker L.P., Wilson D.B. Activity studies of eight purified cellulases Specificity, synergism, and binding domain effects . Biotechnol. Bioeng. 42:1993;1002-1013.
    • (1993) Biotechnol. Bioeng. , vol.42 , pp. 1002-1013
    • Irwin, D.C.1    Spezio, M.2    Walker, L.P.3    Wilson, D.B.4
  • 23
    • 84943470296 scopus 로고
    • Measurement of cellulase activities. (Recommendations of Commission on Biotechnology, IUPAC)
    • Ghose T.K. Measurement of cellulase activities. (Recommendations of Commission on Biotechnology, IUPAC). Pure Appl. Chem. 59:1987;257-268.
    • (1987) Pure Appl. Chem. , vol.59 , pp. 257-268
    • Ghose, T.K.1
  • 24
    • 0026545567 scopus 로고
    • Assay of reducing end-groups in oligosaccharide homologues with 2,2′-bicinchoninate
    • Doner L.W., Irwin P.L. Assay of reducing end-groups in oligosaccharide homologues with 2,2′-bicinchoninate. Anal. Biochem. 202:1992;50-53.
    • (1992) Anal. Biochem. , vol.202 , pp. 50-53
    • Doner, L.W.1    Irwin, P.L.2
  • 25
    • 0027132261 scopus 로고
    • Assessment of endo-1,4-beta-D-glucanase activity by a rapid colorimetric assay using disodium-2,2′-bicinchoninate
    • Garcia E., Johnston D., Whitaker J.R., Shoemaker S.P. Assessment of endo-1,4-beta-D-glucanase activity by a rapid colorimetric assay using disodium-2,2′-bicinchoninate. J. Food Biochem. 17:1993;135-145.
    • (1993) J. Food Biochem. , vol.17 , pp. 135-145
    • Garcia, E.1    Johnston, D.2    Whitaker, J.R.3    Shoemaker, S.P.4
  • 26
    • 0027498262 scopus 로고
    • Light scattering and the absolute characterization of macromolecules
    • Wyatt P.J. Light scattering and the absolute characterization of macromolecules. Anal. Chim. Acta. 272:1993;1-40.
    • (1993) Anal. Chim. Acta , vol.272 , pp. 1-40
    • Wyatt, P.J.1
  • 27
    • 0028763149 scopus 로고
    • The use of GPC coupled with a multiangle laser light scattering photometer for the characterization of polymers. On the determination of molecular weight, size, and branching
    • Podzimek S. The use of GPC coupled with a multiangle laser light scattering photometer for the characterization of polymers. On the determination of molecular weight, size, and branching. J. Appl. Polym. Sci. 54:1994;91-103.
    • (1994) J. Appl. Polym. Sci. , vol.54 , pp. 91-103
    • Podzimek, S.1
  • 28
    • 0024036635 scopus 로고
    • Polysaccharide characterization by aqueous size exclusion chromatography and low-angle laser light scattering
    • Corona A., Rollings J.E. Polysaccharide characterization by aqueous size exclusion chromatography and low-angle laser light scattering. Separation Sci. Technol. 23:1988;855-874.
    • (1988) Separation Sci. Technol. , vol.23 , pp. 855-874
    • Corona, A.1    Rollings, J.E.2
  • 29
    • 0028955385 scopus 로고
    • Comparison of the carbohydrate biological response modifiers Krestin, schizophyllan, and glucan phosphate by aqueous size exclusion chromatography with in-line argon-ion multi-angle laser light scattering photometry and differential viscometry detectors
    • Muller A., Pretus H.A., McNamee R.B., Jones E.L., Browder I.W., Williams D.L. Comparison of the carbohydrate biological response modifiers Krestin, schizophyllan, and glucan phosphate by aqueous size exclusion chromatography with in-line argon-ion multi-angle laser light scattering photometry and differential viscometry detectors. J. Chromatogr. B. 666:1995;283-290.
    • (1995) J. Chromatogr. B. , vol.666 , pp. 283-290
    • Muller, A.1    Pretus, H.A.2    McNamee, R.B.3    Jones, E.L.4    Browder, I.W.5    Williams, D.L.6
  • 30
    • 0026541480 scopus 로고
    • Characterization of polymers by size exclusion chromatography using multiple detection. Investigations on the determination of structural differences of hydroxyethyl starches
    • Sommermeyer K., Cech F., Pfitzer E., Roßler K. Characterization of polymers by size exclusion chromatography using multiple detection. Investigations on the determination of structural differences of hydroxyethyl starches. Chromatographia. 33:1992;151-153.
    • (1992) Chromatographia , vol.33 , pp. 151-153
    • Sommermeyer, K.1    Cech, F.2    Pfitzer, E.3    Roßler, K.4
  • 31
    • 0027473878 scopus 로고
    • Characterization of chitosans via coupled size-exclusion chromatography and multiple-angle laser light-scattering technique
    • Beri R.G., Walker J., Reese E.T., Rollings J.E. Characterization of chitosans via coupled size-exclusion chromatography and multiple-angle laser light-scattering technique. Carbohydr. Res. 238:1993;11-26.
    • (1993) Carbohydr. Res. , vol.238 , pp. 11-26
    • Beri, R.G.1    Walker, J.2    Reese, E.T.3    Rollings, J.E.4
  • 33
    • 0023979454 scopus 로고
    • Light scattering experiments to follow the degradation process of hydroxethyl-cellulose due to Trichoderma reesei cellulase
    • Demeester J., Eigner W.-D., Huber A., Glatter O. Light scattering experiments to follow the degradation process of hydroxethyl-cellulose due to Trichoderma reesei cellulase. J. Wood Chem. Technol. 8:1988;135-153.
    • (1988) J. Wood Chem. Technol. , vol.8 , pp. 135-153
    • Demeester, J.1    Eigner, W.-D.2    Huber, A.3    Glatter, O.4
  • 34
    • 0023644872 scopus 로고
    • Low-angle laser light scattering-aqueous size exclusion chromatography of polysaccharides: Molecular weight distribution and polymer branching determination
    • Yu L.-P., Rollings J.E. Low-angle laser light scattering-aqueous size exclusion chromatography of polysaccharides Molecular weight distribution and polymer branching determination . J. Appl. Polym. Sci. 33:1987;1909-1921.
    • (1987) J. Appl. Polym. Sci. , vol.33 , pp. 1909-1921
    • Yu, L.-P.1    Rollings, J.E.2
  • 36
    • 0026477467 scopus 로고
    • Biochemistry and genetics of actinomycete cellulases
    • Wilson D.B. Biochemistry and genetics of actinomycete cellulases. Crit. Rev. Biotechnol. 12:1992;45-63.
    • (1992) Crit. Rev. Biotechnol. , vol.12 , pp. 45-63
    • Wilson, D.B.1
  • 38
    • 0000709747 scopus 로고
    • Synergism between enzymes involved in the solubilization of native cellulose
    • R.D. Jr. Brown, Jurasek L. Washington, DC: American Chemical Society
    • Wood T.M., McCrae S.I. Synergism between enzymes involved in the solubilization of native cellulose. Brown R.D. Jr, Jurasek L. Hydrolysis of Cellulose Mechanisms of Enzymatic and Acid Catalysis . Vol. 181:1979;181-209 American Chemical Society, Washington, DC.
    • (1979) Hydrolysis of Cellulose: Mechanisms of Enzymatic and Acid Catalysis , vol.181 , pp. 181-209
    • Wood, T.M.1    McCrae, S.I.2
  • 39
    • 0017031327 scopus 로고
    • Enzymatic studies on a cellulase system of Trichoderma viride. IV. Purification and properties of a less-random type cellulase
    • Okada G. Enzymatic studies on a cellulase system of Trichoderma viride. IV. Purification and properties of a less-random type cellulase. J. Biochem. 80:1976;913-922.
    • (1976) J. Biochem. , vol.80 , pp. 913-922
    • Okada, G.1
  • 40
    • 0022311468 scopus 로고
    • Purification and characterization of two β-1,4-endoglucanases from Thermomonospora fusca
    • Calza R.E., Irwin D.C., Wilson D.B. Purification and characterization of two β-1,4-endoglucanases from Thermomonospora fusca. Biochemistry. 24:1985;7797-7804.
    • (1985) Biochemistry , vol.24 , pp. 7797-7804
    • Calza, R.E.1    Irwin, D.C.2    Wilson, D.B.3


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