Expression of Bacterial Chorismate Pyruvate-Lyase in Tobacco: Evidence for the Presence of Chorismate in the Plant Cytosol

Plant and Cell Physiology

Volumn 39, Issue 11, 1998, Pages 1240-1244

  Sommer, Susanne ^a   Heide, Lutz ^a  

^a UNIVERSITY OF TÜBINGEN   (Germany)

Author keywords

4 Hydroxybenzoic acid; Chorismate; Nicotiana tabacum; ubiC

Indexed keywords

CHORISMATE PYRUVATE LYASE; LYASE; RECOMBINANT PROTEIN;

ARTICLE; BIOSYNTHESIS; CONSENSUS SEQUENCE; ENZYMOLOGY; ESCHERICHIA COLI; GENETICS; NUCLEOTIDE SEQUENCE; PLANT; RHIZOBIUM RADIOBACTER; SEQUENCE ALIGNMENT; TOBACCO; TRANSGENIC PLANT;

BASE SEQUENCE; CONSENSUS SEQUENCE; ESCHERICHIA COLI; OXO-ACID-LYASES; PLANTS, GENETICALLY MODIFIED; PLANTS, TOXIC; RECOMBINANT PROTEINS; RHIZOBIUM RADIOBACTER; SEQUENCE ALIGNMENT; TOBACCO;

EID: 0032197306     PISSN: 00320781     EISSN: None     Source Type: Journal    
DOI: 10.1093/oxfordjournals.pcp.a029326     Document Type: Article

References (25)

1. Bar-Peled, M., Bassham, D.C. and Raikhel, N.V. (1996) Transport of proteins in eukaryotic cells: more questions ahead. Plant Mol. Biol. 32: 223-249.
2. Baulcombe, D.C., Saunders, G.R., Bevan, M.W., Mayo, M.A. and Harrison, B.D. (1986) Expression of biologically active viral satellite RNA from the nuclear genome of transformed plants. Nature 321: 446-449.
3. Bauman, G., Raschke, E., Bevan, M. and Schöffl, F. (1987) Functional analysis of sequences required for transcriptional activation of a soybean heat shock gene in transgenic tobacco plants. EMBO J. 6: 1161-1166.
4. Bullock, W.O., Fernandez, J.M. and Short, J.M. (1987) XL1-Blue: a high efficiency plasmid transforming recA Escherichia coli strain with beta-galactosidase selection. Biotechniques 5: 376-378.
5. Eberhard, J., Ehrler, T.T., Epple, P., Felix, G., Raesecke, H.-R., Amrhein, N. and Schmid, J. (1996) Cytosolic and plastidic chorismate mutase isozymes from A rabidopsis thaliana: Molecular characterization and enzymatic properties. Plant J. 10: 815-821.
6. Forlani, G., Parisi, B. and Nielsen, E. (1994) 5-enol-Pyruvyl-shikimate-3-phosphate synthase from Zea mays cultured cells. Plant Physiol. 105: 1107-1114.
7. Gatz, C., Frohberg, C. and Wendenburg, R. (1992) Stringent repression and homogenous de-repression by tetracycline of a modified CaMV 35S promoter in intact transgenic tobacco plants. Plant J. 2: 397-404.
8. Gatz, C., Kaiser, A. and Wendenburg, R. (1991) Regulation of a modified CaMV 35S promoter by the Tn10-encoded Tet represser in transgenic tobacco. Mol. Gen. Genet. 227: 229-237.
9. Hain, R., Reif, H.-J., Krause, E., Langebartels, R., Kindl, H., Vornam, B., Wiese, W., Schmelzer, E., Schreier, P.H., Stöcker, R.H. and Stenzel, K. (1993) Disease resistance results from foreign phytoalexin expression in a novel plant. Nature 361: 153-156.
10. Heide, L., Severin, K. and Siebert, M. (1997) US Patent 08/809,896.
11. Hoekema, A., Hirsch, P.R., Hooykaas, P.J.J. and Schilperoort, R.A. (1983) A binary plant vector strategy based on separation of vir- and T-region of the Agrobacterium tumefaciens Ti-plasmid. Nature 303: 179-180.
12. Höfgen, R. and Willmitzer, L. (1988) Storage of competent cells for Agro-bacterium transformation. Nucl. Acids Res. 16: 9877.
13. Hrazdina, G. and Jensen, R.A. (1992) Spatial organization of enzymes in plant metabolic pathways. Annu. Rev. Plant Physiol. 43: 241-267.
14. Joshi, C.P. (1987) An inspection of the domain between putative TATA box and translation start site in 79 plant genes. Nucl. Acids Res. 15: 6643-6653.
15. Li, S.-M., Wang, Z.-X., Wemakor, E. and Heide, L. (1997) Metabolization of the artificial secondary metabolite 4-hydroxybenzoate in ubiC-transformed tobacco. Plant Cell Rep. 38: 844-850.
16. Logeman, J., Schell, J. and Willmitzer, L. (1987) Improved method for the isolation of RNA from plant tissues. Anal. Biochem. 163: 16-20.
17. Lütcke, H.A., Chow, K.C., Mickel, F.S., Moss, K.A., Kern, H.F. and Scheele, G.A. (1987) Selection of AUG initiation codons differs in plants and animals. EMBO J. 6: 43-48.
18. Murashige, T. and Skoog, F. (1962) A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiol. Plant. 15: 473-497.
19. Schmid, J. and Amrhein, N. (1995) Molecular organization of the shikimate pathway in higher plants. Phytochemistry 39: 737-749.
20. Siebert, M. (1994) Gentechnologische Verkürzung des pflanzlichen Biosyntheseweges für 4-Hydroxybenzoesäure durch Expression des bakteriellen ubiC-Gens in transgenen Tabakpflanzen. PhD Thesis, Freiburg University, Germany.
21. Siebert, M., Bechthold, A., Melzer, M., May, U., Berger, U., Schröder, G., Schröder, J., Severin, K. and Heide, L. (1992) Ubiquinone biosynthesis: cloning of the genes coding for chorismate pyruvate-lyase and 4-hydroxybenzoate octaprenyl transferase from Escherichia coli. FEBS Lett. 307: 347-350.
22. Siebert, M., Sommer, S., Li, S.-M., Wang, Z.-X., Severin, K. and Heide, L. (1996) Genetic engineering of plant secondary metabolism. Accumulation of 4-hydroxybenzoate glucosides as a result of the expression of the bacterial ubiC gene in tobacco. Plant Physiol. 112: 811-819.
23. Sommer, S., Siebert, M., Bechthold, A. and Heide, L. (1998) Specific induction of secondary product formation in transgenic plant cell cultures by use of an inducible promoter. Plant Cell Rep. 17: 891-896.
24. Young, I.G., Langman, L., Luke, R.K. and Gibson, F. (1971). Biosynthesis of the iron-transport compound enterochelin: mutants of Escherichia coli unable to synthesize 2,3-dihydroxybenzoate. J. Bacteriol. 106: 51-57.
25. Yun, D.-J., Hashimoto, T. and Yamada, Y. (1992) Metabolic engineering of medicinal plants. Transgenic Atropa belladonna with an improved alkaloid composition. Proc. Natl. Acad. Sci. USA 89: 11799-11803.