Primed in situ labeling (PRINS): A method for rapid identification and quantification of human chromosomes in both lymphocytes and sperm nuclei

Genome

Volumn 41, Issue 5, 1998, Pages 739-741

  Musio, Antonio ^a   Baroli, Paolo ^b   Sbrana, Isabella ^a  

^a UNIVERSITY OF PISA   (Italy)

^b Laboratorio TOMA   (Italy)

Author keywords

Chromosome X; Human sperm; PRINS

Indexed keywords

CHROMOSOME; IN SITU LABELING; INTERPHASE; LYMPHOCYTE; METAPHASE; SATELLITE DNA; SPERMATID NUCLEUS;

EID: 0032193719     PISSN: 08312796     EISSN: None     Source Type: Journal    
DOI: 10.1139/g98-065     Document Type: Article

References (9)

1. Adams, M.D., Kelley, J.M., Gocayne, J.D., Dubnick, M., Polymeropoulos, M.H., Xiao, H., Merril, C.R., Wu, A., Olde, B., Moreno, R.F., Kerlavage, A.R., McCombie, W.R., and Venter, J.C. 1991. Complementary DNA sequencing: expressed sequence tags and human genome project. Science (Washington, D.C.), 252: 1651-1656.
2. Bottema, C.D.K., and Sommer, S.S. 1993. PCR amplification of specific alleles: rapid detection of known mutations and polymorphism. Mutat. Res. 288: 93-102.
3. Cinti, C., Santi, S., and Maraldi, N. 1993. Localization of single copy gene by PRINS technique. Nucleic Acids Res. 21: 5799-5800.
4. Gosden, J., Hanratty, D., Starling, J., Fantes, J., Mitchell, A., and Porteus, D. 1991. Oligonucleotide primed in situ DNA synthesis (PRINS): a method for chromosome mapping, banding and investigation of sequence organization. Cytogenet. Cell Genet. 57: 100-104.
5. Guttenbach, M., Schakowski, K., and Schmid, M. 1994. Incidence of chromosome 3, 7, 10, 17 and X disomy in mature human sperm nuclei as determined by non radioactive in situ hybridization. Hum. Genet. 93: 7-12.
6. Lebo, R.V., Flandermeyer, R.R., Diukman, R., Lynch, E.D., Lepercq, J.A., and Golbus, M.S. 1992. Prenatal diagnosis with repetitive in situ hybridization probes. Am. J. Med. Genet. 43: 848-854.
7. Musio, A., and Rainaldi, G. 1997. Cycling-PRINS. A method to improve the accuracy of telomeric detection in mammalian chromosomes. Mutat. Res. 390: 1-4.
8. Pellestor, F., Girardet, A., Coignet, L., Andreo, B., and Charlieu, J.P. 1996. Assessment of aneuploidy for chromosomes 8, 9, 13, 16, and 21 in human sperm by using primed in situ labeling technique. Am. J. Hum. Genet. 58: 797-802.
9. Waye, J.J., and Willard, H.F. 1987. Nucleotide sequence heterogeneity of alpha satellite repetitive DNA: a survey of alphoid sequences from different human chromosomes. Nucleic Acids Res. 15: 7549-7562.