Cloning, nucleotide sequence and expression of the gene encoding factor Xa inhibitor from the salivary glands of the tick, Ornithodoros savignyi

Experimental and Applied Acarology

Volumn 22, Issue 10, 1998, Pages 603-619

  Joubert, A M ^a   Louw, A I ^a   Joubert, F ^a   Neitz, A W H ^a  

^a UNIVERSITY OF PRETORIA   (South Africa)

Author keywords

Anticoagulant; Expression; fXa inhibitor; Ornithodoros savignyi; Sequence; Ticks

Indexed keywords

AMINO ACID SEQUENCE; AMINO ACID; BACULOVIRUS EXPRESSION SYSTEM; BLOOD CLOTTING FACTOR 10A INHIBITOR; CLONING; COMPLEMENTARY DNA; GENE EXPRESSION; GENE SPECIFIC PRIMER; MOLECULAR WEIGHT; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION;

AMINO ACID SEQUENCE; ANIMALS; ANTICOAGULANTS; BASE SEQUENCE; CLONING, MOLECULAR; DNA, COMPLEMENTARY; FACTOR XA; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; PEPTIDES; PROTEIN CONFORMATION; RECOMBINANT PROTEINS; SALIVARY GLANDS; SEQUENCE ANALYSIS, DNA; SEQUENCE HOMOLOGY, AMINO ACID; SERINE PROTEINASE INHIBITORS; TICKS;

ACARI; ARGASIDAE; HEXAPODA; INSECTA; ORNITHODOROS MOUBATA; ORNITHODOROS SAVIGNYI; UNIDENTIFIED BACULOVIRUS;

EID: 0032192297     PISSN: 01688162     EISSN: None     Source Type: Journal    
DOI: 10.1023/A:1006198713791     Document Type: Article

References (37)

1. Altschul, S.F., Gish, W., Miller, W., Myers, E.W. and Lipman, D.J. 1990. Basic local alignment search tool. J. Mol. Biol. 215: 403-410.
2. Anderson, D., Harris, R., Polayes, D., Ciccarone, V., Donahue, R., Gerard, G., Jessee, J. and Luckow, V. 1995. Rapid generation of recombinant baculovirus and expression of foreign genes using the BAC-TO BAC™ baculovirus expression system. Focus 17: 40-45.
3. Antuch, W., Güntert, P., Billeter, M., Hawthorne, T., Grossenbacher, H. and Wuthrich, K. 1994. NMR solution structure of the recombinant tick anticoagulant protein (rTAP), a factor Xa inhibitor from the tick Ornithodorus moubata. FEBS Lett. 352: 251-257.
4. Bairoch, A., Bucher, P. and Hofmann, K. 1995. The PROSITE database, its status in 1995. Nucleic Acids Res. 24: 189-196.
5. Beverley, S.M. 1992. Enzymatic amplification of RNA by PCR. In Short protocols in molecular biology, F.M. Ausubel, R. Brent, R.E. Kingston, D.D. Moore, J.G. Seidman, J.A. Smith and K. Struhul (eds), pp. 15-13. Greene Publishing Associates and John Wiley & Sons, New York.
6. Capello, M., Clyne, L.P., McPherdran, P. and Hotez, P.J. 1993. Ancylostoma factor Xa inhibitor: partial purification and its identification as a major hookworm-derived anticoagulant in vitro. J. Infect. Dis. 167: 1474-1477.
7. Condra, D., Nutt, E., Christopher, J.P., Simpson, E., Friedman, P.A. and Jacobs, J.W. 1989. Isolation and structural characterization of a potent inhibitor of coagulation factor Xa from the leech Haementeria ghilianni. Thromb. Haemost. 61: 437-441.
8. Creedon, K.A., Rathod, P.K. and Wellems, T.E. 1994. Plasmodium falciparum S-adenosylhomocysteine hydrolase. cDNA identification, predicted protein sequence and expression in Escherichia coli. J. Biol. Chem. 269: 16364-16370.
9. Davies, A.H. 1995. Advances in the use of recombinant baculoviruses for the expression of heterologous proteins. Curr. Opin. Biotechnol. 6: 543-547.
10. Dietmaier, W., Fabry, S. and Schmitt, R. 1993. DISEC-TRISEC: di- and trinucleotide-sticky-end cloning of PCR-amplified DNA. Nucleic Acids Res. 21: 3603-3604.
11. Dunwiddie, C., Nutt, E.M., Vlasuk, G.P., Siegl, P.K.S. and Schaffer, L.W. 1992. Anticoagulant efficacy and immunogenecity of the selective factor Xa inhibitor antistasin following subcutaneous administration in the rhesus monkey. Thromb. Haemost. 67: 371-376.
12. Gaspar, A.R.M.D., Crause, J.C. and Neitz, A.W.H. 1995. Identification of anticoagulant activities in the salivary glands of the soft tick, Ornithodoros savignyi. Exp. Appl. Acarol. 19: 117-126.
13. Gaspar, A.R.M.D., Joubert, A.M., Crause, J.C. and Neitz, A.W.H. 1996. Isolation and characterization of an anticoagulant from the salivary glands of the tick, Ornithodoros savignyi (Acari: Argasidae). Exp. Appl. Acarol. 20: 583-598.
14. Geourjon, C., Deleage, G. and Roux, B. 1991. ANTHEPROT: an interactive graphics software for analyzing protein structures from sequences. J. Mol. Graph. 9: 188-190.
15. Harker, L.A. 1994. Strategies for inhibiting the effects of thrombin. Blood Coagul. Fibrinolysis 5: S47-S58.
16. Jacobs, J.W., Cupp, E.W., Sardana, M. and Friedman, P.A. 1990. Isolation and characterization of a coagulation factor Xa inhibitor from black fly salivary glands. Thromb. Haemost. 64: 235-238.
17. Joubert, A.M., Crause, J.C., Gaspar, A.R.M.D., Clarke, F.C., Spickett, A.M. and Neitz, A.W.H. 1995. Isolation and characterization of an anticoagulant present in the salivary glands of the bont-legged tick, Hyalomma truncatum. Exp. Appl. Acarol. 19: 79-92.
18. Kozak, M. 1987. An analysis of 5′-noncoding sequence from 699 vertebrate messenger RNAs. Nucleic Acids Res. 15: 8125-8130.
19. Kyte, J. and Doolittle, R.F. 1982. A simple method of displaying the hydropathic character of a protein. J. Mol. Biol. 157: 105-132.
20. Laemmli, U.K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680-685.
21. Limo, M.K., Voigt, W.P., Tumbo-Oeri, A.G., Njogi, R.M. and Ole-Moi, O.K. 1991. Purification and characterization of an anticoagulant from the salivary glands of the ixodid tick, Rhipicephalus appendiculatus. Exp. Parasitol. 72: 418-429.
22. Neeper, M.P., Waxman, L., Smith, D.E., Schulman, C.A., Sardana, M., Ellis, R.W., Schaeffer, L.W., Siegel, P.K.S. and Vlasuk, G.P. 1990. Characterization of recombinant tick anti-coagulant peptide. J. Biol. Chem. 165: 17746-17752.
23. Nutt, E., Gasic, T., Rodkey, J., Gasic, G.J., Jacobs, J.W., Friedman, P.A. and Simpson, E. 1988. The amino acid sequence of antistasin. J. Biol. Chem. 263: 10162-10167.
24. Ohta, N. 1994. Interaction of antistasin-related peptides with factor Xa: identification of a core inhibitory sequence. Thromb. Haemost. 72: 825-830.
25. O'Reilly, D.R., Miller, L.K. and Luckow, V.A. 1992. An overview of baculoviruses, choosing a transfer plasmid and parent virus, methods for vector construction and gene expression. In Baculo expression vectors: a laboratory manual, D.R. O'Reilly, L.K. Miller and V.A. Luckow (eds), pp. 1-236. W.H. Freeman & Company, New York.
26. Ribeiro, J.M.C. 1987. Role of saliva in blood-feeding by arthropods. Ann. Rev. Entomol. 32: 463-478.
27. Ribeiro, J.M.C., Sarkis, J.F.F., Rossignol, P.A. and Spielman, A. 1984. Salivary apyrase of Aedes aegypti: characterization and secretory fate. Comp. Biochem. Physiol. 79B: 81-86.
28. Sali, A. and Blundell, T.L. 1993. Comparative protein modelling by satisfaction of spatial restraints. J. Mol. Biol. 234: 799-815.
29. Sanyal, G., Marquis-Omer, D., Waxman, L., Mach, H., Ryan, J.A., O'Brien Gress, J. and Middaugh, C.R. 1995. Spectroscopic characterization of tick anticoagulant peptide. Biochem. Biophys. Acta 1249: 100-108.
30. Schaefer, B.C. 1995. Revolutions in rapid amplification of cDNA ends: new strategies for polymerase chain reaction cloning of full length cDNA ends. Anal. Biochem. 227: 255-273.
31. Smartt, C.T., Kim, A.P., Grossman, G.L. and James, A.A. 1995. The apyrase gene of the vector mosquito, Aedes aegypti is expressed specifically in the adult female salivary glands. Exp. Parasitol. 81: 239-248.
32. Smith, S.W., Overbeek, R., Woese, C.R., Gilbert, W. and Gillevet, P.M. 1994. The genetic data environment an expandable GUI for multiple sequence-analysis. Comput. Appl. Biosci. 10: 671-675.
33. Thompson, J.D., Higgins, D.G. and Gibson, T.J. 1994. CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res. 22: 4673-4680.
34. Titus, R.G. and Ribeiro, J.M.C. 1990. The role of vector saliva in transmission of arthropod-borne disease. Parasitol. Today. 6: 157-160.
35. Treco, D.A. 1992. Phenol extraction and concentration of DNA from aqueous solutions. In Short protocols in molecular biology, F.M. Ausubel, R. Brent, R.E. Kingston, D.D. Moore, J.G. Seidman, J.A. Smith and K. Struhl (eds), pp. 2-4-2-5. Greene Publishing Associates and John Wiley & Sons, New York.
36. Waxman, L. and Connolly, T.M. 1993. Isolation of an inhibitor selective for collagen-stimulated platelet aggregation from the soft tick, Ornithodoros moubata. J. Biol. Chem. 268: 5445-5449.
37. Waxman, L., Smith, D.E., Arcuri, K.E. and Vlasuk, G.P. 1990. Tick anticoagulant peptide is a novel inhibitor of blood coagulation factor Xa. Science 248: 593-596.