Expression and ligand binding assays of soluble cytokine receptor-immunoglobulin fusion proteins

Protein Expression and Purification

Volumn 14, Issue 1, 1998, Pages 120-124

  Brown, Steven J ^a   Becherer, Kathleen A ^a   Blumeyer, Kirsten ^a   Kautzer, Curtis ^a   Axelrod, Fumiko ^a   Le, Huong ^a   McConnell, Stephen J ^a   Whalley, Alice ^a   Spinella, Dominic G ^a  

^a Chugai Biopharmaceuticals Inc   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BINDING AFFINITY; CLONING VECTOR; CYTOKINE RECEPTOR; ENZYME LINKED IMMUNOSORBENT ASSAY; EXPRESSION VECTOR; HYBRID PROTEIN; IMMUNOGLOBULIN G; IMMUNOGLOBULIN RECEPTOR; LIGAND BINDING; NITRIC OXIDE; PROTEIN EXPRESSION; PROTEIN PURIFICATION; WESTERN BLOTTING;

RODENTIA;

EID: 0032189359     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1006/prep.1998.0940     Document Type: Article

References (21)

1. Beck J. T., Marsters S. A., Harris R. J., Carter P., Ashkenazi A., Chamow S. M. Generation of soluble interleukin-1 receptor from an immunoadhesin by specific cleavage. Mol. Immunol. 31:1994;1335-1344.
2. Beckmann M. P., Cosman D., Fanslow W., Maliszewski C. R., Lyman S. D. The interleukin-4 receptor: Structure, function, and signal transduction. Chem. Immunol. 51:1992;107-134.
3. Broudy V. C., Lin N., Brice M., Nakamoto B., Papayannopoulou T. Erythropoietin receptor characteristics on primary human erythroid cells. Blood. 77:1991;2583-2590.
4. Broudy V. C., Lin N., Egrie J., de Haen C., Weiss T., Papayannopoulou T., Adamson J. W. Identification of the receptor for erythropoietin on human and murine erythroleukemia cells and modulation by phorbol ester and dimethyl sulfoxide. Proc. Natl. Acad. Sci. USA. 85:1988;6513-6517.
5. Crowe P. D., VanArsdale T. L., Walter B. N., Dahms K. M., Ware C. F. Production of lymphotoxin (LT alpha) and a soluble dimeric form of its receptor using the baculovirus expression system. J. Immunol. Methods. 168:1994;79-89.
6. Fountoulakis M. Apparent heterogeneity of recombinant interferon gamma receptors produced in prokaryotic and eukaryotic expression systems. J. Chem. Technol. Biotechnol. 65:1996;123-130.
7. Fountoulakis M., Mesa C., Schmid G., Gentz R., Manneberg M., Zulauf M., Dembic Z., Garotta G. Interferon gamma receptor extracellular domain expressed as IgG fusion protein in Chinese hamster ovary cells. Purification, biochemical characterization, and stoichiometry of binding. J. Biol. Chem. 270:1995;3958-3964.
8. Gascoigne N. R. J., Goodnow C. C., Dudzik K. I., Oi V. T., Davis M. M. Secretion of a chimeric T-cell receptor-immunoglobulin protein. Proc. Natl. Acad. Sci. USA. 84:1987;2936-2940.
9. Idzerda R. L., March C. J., Mosley B., Lyman S. D., Bos T. V., Gimpel S. D., Din W. S., Grabstein K. H., Widmer M. B., Park L. S., Cosman D., Beckmann M. P. Human interleukin 4 receptor confers biological responsiveness and defines a novel receptor superfamily. J. Exp. Med. 171:1990;861-873.
10. Jones S. S., D'Andrea A. D., Haines L. L., Wong G. G. Human erythropoietin receptor: cloning, expression, and biologic characterization. Blood. 76:1990;31-35.
11. Karlsson R., Michaelsson A., Mattsson L. Kinetic analysis of monoclonal antibody-antigen interactions with a new biosensor based analytical system. J. Immunol. Methods. 145:1991;229-240.
12. Kozak M. Point mutations define a sequence flanking the AUG initiator codon that modulates translation by eukaryotic ribosomes. Cell. 44:1986;283-292.
13. Loetscher H., Pan Y. C., Lahm H. W., Gentz R., Brockhaus M., Tabuchi H., Lesslauer W. Molecular cloning and expression of the human 55 kd tumor necrosis factor receptor. Cell. 61:1990;351-359.
14. Matzuk M. Human Cytokines: Their Role in Disease and Therapy. 1995;Blackwell Science, Cambridge.
15. Niitsu Y., Watanabe N., Sone H., Neda H., Yamauchi N., Maeda M., Urushizaki I. Analysis of the TNF receptor on KYM cells by binding assay and affinity cross-linking. J. Biol. Response Mod. 7:1988;276-282.
16. Philo J. S., Aoki K. H., Arakawa T., Narhi L. O., Wen J. Dimerization of the extracellular domain of the erythropoietin (EPO) receptor by EPO: One high-affinity and one low-affinity interaction. Biochemistry. 35:1996;1681-1691.
17. Taremi S. S., Prosise W. W., Rajan N., O'Donnell R. A., Le H. V. Human interleukin 4 receptor complex: Neutralization effect of two monoclonal antibodies. Biochemistry. 35:1996;2322-2331.
18. Ward L. D., Howlett G. J., Hammacher A., Weinstock J., Yasukawa K., Simpson R. J., Winzor D. J. Use of a biosensor with surface plasmon resonance detection for the determination of binding constants: Measurement of interleukin-6 binding to the soluble interleukin-6 receptor. Biochemistry. 34:1995;2901-2907.
19. Yamasaki K., Taga T., Hirata Y., Yawata H., Kawanishi Y., Seed B., Taniguchi T., Hirano T., Kishimoto T. Cloning and expression of the human interleukin-6 (BSF-2/IFN beta 2) receptor. Science. 241:1988;825-828.
20. Yanofsky S. D., Baldwin D. N., Butler J. H., Holden F. R., Jacobs J. W., Balasubramanian P., Chinn J. P., Cwirla S. E., Peters-Bhatt E., Whitehorn E. A., E. H. T., Akeson A., Bowlin T. L., Dower W. J., Barrett R. W. High affinity type I interleukin 1 receptor antagonists discovered by screening recombinant peptide libraries. Proc. Natl. Acad. Sci. USA. 93:1996;7381-7386.
21. Yet M. G., Jones S. S. The extracytoplasmic domain of the erythropoietin receptor forms a monomeric complex with erythropoietin. Blood. 82:1993;1713-1719.