Unambiguous Correlations of Backbone Amide and Aliphatic Gamma Resonances in Deuterated Proteins

Journal of Magnetic Resonance

Volumn 134, Issue 2, 1998, Pages 350-354

  McCallum, Scott A ^a   Hitchens, T K ^b   Rule, Gordon S ^a,b  

^a UNIVERSITY OF VIRGINIA   (United States)

^b CARNEGIE MELLON UNIVERSITY   (United States)

Author keywords

Gamma carbon; HN(CACB)CG; HN(COCACB)CG; Perdeuterated protein; Pulse sequence

Indexed keywords

AMIDE; DEUTERIUM; GLUTATHIONE TRANSFERASE; PROTEIN;

ARTICLE; HUMAN; IMAGE PROCESSING; INSTRUMENTATION; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY;

AMIDES; DEUTERIUM; GLUTATHIONE TRANSFERASE; HUMANS; IMAGE PROCESSING, COMPUTER-ASSISTED; MAGNETIC RESONANCE SPECTROSCOPY; PROTEINS;

EID: 0032185553     PISSN: 10907807     EISSN: None     Source Type: Journal    
DOI: 10.1006/jmre.1998.1525     Document Type: Editorial

References (24)

1. 2 H-labeled proteins: Application to a 37-kDa TRP repressor-DNA complex, J. Am. Chem. Soc. 116, 6464-6465 (1994).
2. 2 H-labeled proteins with high sensitivity, J. Am. Chem. Soc. 116, 11,655-11,666 (1994).
3. D. A. Torchia, S. W. Sparks, and A. Bax, Delineation of α-helical domains in deutered Staphylococcal nuclease by 2D NOE NMR spectroscopy, J. Am. Chem. Soc. 110, 2320-2321 (1988).
4. D. M. LeMaster, Deuterium labelling in NMR structural analysis of larger proteins, Quart. Rev. Biophys. 23, 133-174 (1990).
5. L. E. Kay, M. Ikura, R. Tschudin, and A. Bax, Three-dimensional triple-resonance NMR spectroscopy of isotopically enriched proteins, J. Magn. Reson. 89, 496-514 (1990).
6. 13 C enriched proteins, J. Biomolec. NMR. 1, 99-104 (1991).
7. D. R. Muhandiram and L. E. Kay, Gradient-enhanced triple-resonance three-dimensional NMR experiments with improved sensitivity, J. Magn. Reson. B 103, 203-216 (1994).
8. α pulses for improvement of HN(CA)CO-D and HN(COCA)NH-D experiments, J. Magn. Reson. B 111, 194-198 (1996).
9. 15 N-enriched proteins. Application to triple resonance 4D J connectivity of sequential amides, J. Am. Chem. Soc. 115, 4369-4370 (1993).
10. 13 C enriched proteins, J. Magn. Reson. 88, 425-431 (1990).
11. G. T. Montelione, B. A. Lyons, S. D. Emerson, and M. J. Tashiro, An efficient triple resonance experiment using carbon-13 Isotropic mixing for determining sequence-specific resonance assignments of isotopically-enriched proteins, J. Am. Chem. Soc. 114, 10,974-10,975 (1992).
12. B. T. Farmer II and R. A. Venters, Assignment of side-chain resonances in perdeuterated proteins, J. Am. Chem. Soc. 117, 4187-4188 (1995).
13. M. Wittekind and L. Mueller, HNCACB, a high sensitivity 3D NMR experiment to correlate amide-proton and nitrogen resonances with alpha- and beta-carbon resonances in proteins, J. Magn. Reson. B 101, 201-205 (1993).
14. A. Bax, M. Ikura, L. E. Kay, D. A. Torchia, and R. Tschudin, Comparison of different modes of two-dimensional reverse-correlation NMR for the study of proteins, J. Magn. Reson. 86, 304-318 (1990).
15. W. R. Vorachek, W. R. Pearson, and G. S. Rule, Cloning, expression, and characterization of a class-mu glutathione transferase from human muscle, the product of the GST4 locus, Proc. Natl. Acad. Sci. 88, 4443-4447 (1991).
16. S. Raghunathan, R. J. Chandross, R. H. Kretzinger, T. J. Allison, C. J. Penington, and G. S. Rule, Crystal structure of human class mu glutathione transferase GSTM2-2. Effects of lattice packing on conformational heterogeneity, J. Mol. Biol. 238, 815-832 (1994).
17. 13 C magnetization, J. Magn. Reson. B 101, 114-119 (1993).
18. 13 C resonances of Interleukin-1 β using double- and triple-resonance heteronuclear three-dimensional NMR spectroscopy, Biochemistry 29, 8172-8184 (1990).
19. A. J. Shaka, C. J. Lee, and A. Pines, Iterative schemes for bilinear operators: Application to spin decoupling, J. Magn. Reson. 77, 274-293 (1988).
20. A. J. Shaka, P. B. Barker, and R. Freeman, Computer-optimized decoupling scheme for wideband applications and low-level operation, J. Magn. Reson. 64, 547-552 (1985).
21. 13 C]carbonyl decoupling, J. Am. Chem. Soc. 114, 2108-2112 (1992).
22. S. Talluri and G. Wagner, An optimized 3D NOESY-HSQC, J. Magn. Reson. B 112, 200-205 (1996).
23. D. Marion, M. Ikura, R. Tschudin, and A. Bax, Rapid recording of 2D NMR spectra without phase cycling. Application to the study of hydrogen exchange in proteins, J. Magn. Reson. 85, 393-399 (1989).
24. D. Marion, M. Ikura, and A. Bax, Improved solvent suppression in one- and two-dimensional NMR spectra by deconvolution of time domain data, J. Magn. Reson. 84, 425-430 (1989).