Simultaneous isotopic and nonisotopic in situ hybridization histochemistry with cRNA probes

Brain Research Protocols

Volumn 3, Issue 1, 1998, Pages 22-32

  Kerner, Julie A ^a   Standaert, David G ^a   Penney Jr , John B ^a   Young, Anne B ^a   Landwehrmeyer, G Bernhard ^b  

^a MASSACHUSETTS GENERAL HOSPITAL   (United States)

^b UNIVERSITY OF FREIBURG   (Germany)

Author keywords

cRNA; Digoxigenin; Gene expression; Histochemistry; In situ hybridization; mRNA; PCR; Radioactive

Indexed keywords

COMPLEMENTARY RNA; DIGOXIGENIN;

ANIMAL TISSUE; ARTICLE; HISTOCHEMISTRY; IN SITU HYBRIDIZATION; ISOTOPE LABELING; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROMOTER REGION; RNA ANALYSIS;

CORPUS STRIATUM; DIGOXIGENIN; HISTOCYTOCHEMISTRY; IN SITU HYBRIDIZATION; NEURONS; POLYMERASE CHAIN REACTION; RECEPTORS, METABOTROPIC GLUTAMATE; RECEPTORS, N-METHYL-D-ASPARTATE; RNA, COMPLEMENTARY; SULFUR RADIOISOTOPES; TIME FACTORS; TRANSCRIPTION, GENETIC;

EID: 0032171043     PISSN: 1385299X     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1385-299X(98)00017-8     Document Type: Article

References (15)

1. Angerer L.M., Angerer R.C. Localization of mRNAs by in situ hybridization. Meth. Cell Biol. 35:1991;37-71.
2. M.F. Chesselet, L.T. Weiss-Wunder, Quantification of in situ hybridization histochemistry, in: J.H. Eberwine, K.L. Valentino, J.D. Barchas (Eds.), In Situ Hybridization in Neurobiology, Oxford University Press, New York, 1994, pp. 114-123.
3. Counihan T.J., Landwehrmeyer G.B., Standaert D.G., Kosinski C.M., Scherzer C.R., Dagget L.P., Veliçelebi G., Young A.B., Penney J.B. Jr. Expression of N-methyl-D-aspartate receptor mRNA in the human brain: mesencephalic dopaminergic neurons. J. Comp. Neurol. 390:1998;91-101.
4. Dagerlind Å., Friberg K., Bean A.J., Hökfelt T. Sensitive mRNA detection using unfixed tissue: combined radioactive and non-radioactive in situ hybridization histochemistry. Histochemistry. 98:1992;39-49.
5. P.C. Emson, B. Heppelmann, S.J., Augood, Development of techniques to combine isotopic and nonisotopic in situ hybridization and immunocytochemistry for phenotypic characterization of individual neurons, in: J.H. Eberwine, K.L. Valentino, J.D. Barchas (Eds.), In Situ Hybridization in Neurobiology, Oxford University Press, New York, 1994, pp. 43-62.
6. Kerner J.A., Standaert D.G., Penney J.B. Jr., Young A.B., Landwehrmeyer G.B. Expression of group one metabotropic glutamate receptor subunit mRNAs in neurochemically identified neurons in the rat neostriatum, neocortex and hippocampus. Mol. Brain Res. 48:1997;259-269.
7. Landwehrmeyer G.B., Standaert D.G., Testa C.M., Penney J.B. Jr., Young A.B. NMDA receptor subunit mRNA expression by projection neurons and interneurons in rat striatum. J. Neurosci. 15:1995;5297-5307.
8. Normand E., Bloch B. Simultaneous detection of two messenger RNAs in the central nervous system: a simple two-step in situ hybridization procedure using a combination of radioactive and non-radioactive probes. J. Histochem. Cytochem. 39:1991;1575-1578.
9. J. Sambrook, E.F. Fritsch, T. Maniatis (Eds.), Molecular Cloning: A Laboratory Manual, Cold Springs Harbor Laboratory Press, 1989.
10. Sitzman J.H., LeMotte P.K. Rapid and efficient generation of PCR-derived riboprobe templates for in situ hybridization histochemistry. J. Histochem. Cytochem. 41:1993;773-3776.
11. Standaert D., Landwehrmeyer G., Kerner J., Penney J.B. Jr., Young A. Expression of NMDAR2D glutamate receptor subunit mRNA in neurochemically-identified interneurons in the rat neostriatum, neocortex, and hippocampus. Mol. Brain Res. 42:1996;89-102.
12. Testa C.M., Standaert D.G., Landwehrmeyer G.B., Penney J.B. Jr., Young A.B. Differential expression of the mGluR5 metabotropic glutamate receptor by rat striatal neurons. J. Comp. Neurol. 354:1995;241-252.
13. Vonsattel J., Aizawa H., Ge P., DiFiglia M., McKee A.C., MacDonald M., Gusella J.F., Landwehrmeyer G.B., Bird E.D., Richardson E.P.J., Hedley-Whyte E.T. An improved approach to prepare human brains for research. J. Neuropathol. Exp. Neurol. 54:1995;42-56.
14. Young W.S. Simultaneous use of digoxigenin- and radiolabeled oligodeoxyribonucleotide probes for hybridization histochemistry. Neuropeptides. 13:1989;271-275.
15. Young W.S. III, Hsu A.C. Observations on the simultaneous use of digoxigenin- and radiolabeled oligodeoxyribonucleotide probes for hybridization histochemistry. Neuropeptides. 18:1991;75.