D-ribulose-5-phosphate 3-epimerase: Cloning and heterologous expression of the spinach gene, and purification and characterization of the recombinant enzyme

Plant Physiology

Volumn 118, Issue 1, 1998, Pages 199-207

  Chen, Yuh Ru ^a   Hartman, Fred C ^b   Lu, Tse Yuan S ^b   Larimer, Frank W ^b  

^a University of Tennessee NICS   (United States)

^b OAK RIDGE NATIONAL LABORATORY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

COMPLEMENTARY DNA; EPIMERASE; PLANT DNA; RECOMBINANT PROTEIN; RIBULOSEPHOSPHATE 3 EPIMERASE; RIBULOSEPHOSPHATE 3-EPIMERASE;

ARTICLE; ENZYME STABILITY; ENZYMOLOGY; GENE; GENE EXPRESSION; GENETICS; ISOLATION AND PURIFICATION; KINETICS; METABOLISM; MOLECULAR CLONING; MOLECULAR GENETICS; MOLECULAR WEIGHT; NUCLEOTIDE SEQUENCE; PROTEIN CONFORMATION; SPINACH; THERMODYNAMICS;

BASE SEQUENCE; CARBOHYDRATE EPIMERASES; CLONING, MOLECULAR; DNA, COMPLEMENTARY; DNA, PLANT; ENZYME STABILITY; GENE EXPRESSION; GENES, PLANT; KINETICS; MOLECULAR SEQUENCE DATA; MOLECULAR WEIGHT; PROTEIN CONFORMATION; RECOMBINANT PROTEINS; SPINACIA OLERACEA; THERMODYNAMICS;

EID: 0032160488     PISSN: 00320889     EISSN: None     Source Type: Journal    
DOI: 10.1104/pp.118.1.199     Document Type: Article

References (6)

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2. Bär J, Naumann M, Reuter R, Kopperschläger G (1996) Improved purification of ribulose 5-phosphate 3-epimerase from Saccharomyces cerevisiae and characterization of the enzyme. Bioseparation 6: 233-241
3. Blattner FR, Burland V, Plunkett G, Sofia HJ, Daniels DL (1993) Analysis of the Escherichia coli genome. IV. DNA sequence of the region from 89.2 to 92.8 minutes. Nucleic Acids Res 21: 5408-5417
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6. Wood T (1979) Purification and properties of D-ribulose-5-phosphate 3-epimerase from calf liver. Biochim Biophys Acta 570: 352-362