Detection of intranucleolar chromatin using an ultrastructural immunolabelling technique

Cell Biology International

Volumn 22, Issue 9-10, 1998, Pages 609-614

  Ghosh, Sibdas ^a,b   Paweletz, Neidhard ^a  

^a GERMAN CANCER RESEARCH CENTER DKFZ   (Germany)

^b UNIVERSITY OF CALCUTTA   (India)

Author keywords

Intranucleolar chromatin; Nucleolar organization; Transcriptional units

Indexed keywords

BROXURIDINE; RIBOSOME DNA; SODIUM CHLORIDE;

ANTIBODY LABELING; ARTICLE; CELL GRANULE; CELL ULTRASTRUCTURE; CHROMATIN; FIBER; HUMAN; HUMAN CELL; IMMUNOCYTOCHEMISTRY; NUCLEOLUS;

EID: 0032157064     PISSN: 10656995     EISSN: None     Source Type: Journal    
DOI: 10.1006/cbir.1998.0265     Document Type: Article

References (24)

1. BUSCH H, SMETANA K, 1970. The Nucleolus. Academic Press, NY.
2. FISCHER D, WEISENBERGER D, SCHEER U, 1991. Assigning functions to nucleolar structures. Chromosoma 101: 133-140.
3. GHOSH S, 1987. The nucleolus. Int Rev Cytol 17 (Suppl.): 573-598.
4. GHOSH S, PAWELETZ N, 1984. The events associated with the initiation of mitosis in multi-nucleate cells. Chromosoma 90: 57-67.
5. GHOSH S, PAWELETZ N, 1987. Active ribosomal cistrons and their primary transcripts located in the nucleolus. Cell Biol Int Rep 11: 205-210.
6. GHOSH S, PAWELETZ N, 1990. Localisation of ribosomal cistrons at the ultrastructural level by in situ hybridisation technique. Cell Biol Int Rep 14: 521-525.
7. GHOSH S, PAWELETZ N, 1992. Localisation of chromatin in 'persistent nucleoli' in okadaic acid treated HeLa cells. Cell Biol Int Rep 16: 475-480.
8. GHOSH S, PAWELETZ N, 1994. Nucleolar organisation as revealed in cycloheximide treated cells. Cell Biol Int 18: 783-789.
9. GHOSH S, PAWELETZ N, 1996. Nucleolar organisation as revealed in cell spreads. Cell Biol Int 20: 465-470.
10. GHOSH S, CHAKRABARTI A, CHAUDHURI SK, 1993. Active ribosomal cistrons and their primary transcripts located in the nucleolus. Cell Biol Int 17: 759-763.
11. GHOSH S, DEY R, SARKAR S, BHATTACHARYA D, 1990. Timings of DNA synthesis in the nucleolus of Allium cepa. Cell Biol Int Rep 14: 173-177.
12. JIMENEZ-GARCIA LF, SEGURA VALDEZ ML, OCHS RL, ECHEVERRIA OM, VASQUEZ-NIN GH, BUSCH H, 1993. Electron microscopic localisation of ribosomal DNA in rat liver nucleoli by non-isotopic in situ hybridisation. Exp Cell Res 207: 220-225.
13. JORDAN EG, 1991. Interpreting nucleolar structure: where are the transcribing genes? J Cell Sci 98: 437-442.
14. LEVY N, NAVARRO A, DEPETRIS D, MORETTI C, STAHL A, 1996. Localisation of ribosomal genes in human spermatogonia by fluorescent in situ hybridisation and confocal microscopy. Cytogenet Cell Genet 73: 290-294.
15. MILLER OL, BEATTY BR, 1969. Visualisation of nucleolar genes. Science 164: 955-957.
16. MIRRE C, STAHL A, 1981. Ultrastructural organisation sites of transcription and distribution of fibrillar centres in the nucleolus of mouse oocytes. J Cell Sci 48: 105-126.
17. PAWELETZ N, SCHROETER D, FINZE EM, 1994. Making first contacts between the spindle and the chromosomes in HeLa cells. Chromosome Res 2: 115-122.
18. SCHÖFER C, WEIPOLTSHAMMER K, ALMEDER M, MULLER M, WACHTLER F, 1996. Redistribution of ribosomal DNA after blocking of transcription induced by actinomycin D. Chromosome Res. 4: 384-391.
19. SCHWARZACHER HG, WACHTLER F, 1993. The nucleolus. Anat Embryol Berl 188: 515-536.
20. SMETANA K, LANE M, BUSCH H, 1966. Studies on nucleoli of leukomic agranulocytes and plasmacytes in multiple myeloma. Exp Mol Pathol 5: 236-244.
21. TSVETKOV AG, PARFENOV VN, 1995. The organisation of dispersed nucleolar chromatin and the ultrastructure of nucleoli in the oogenesis of the common frog. Tsitologia 37: 567-573.
22. THIRY M, SCHEER U, GOESSENS G, 1991. Localisation of nucleolar chromatin by immunocytochemistry and in situ hybridisation at the electron microscope level. Electron Microsc Rev 4: 85-110.
23. VANDELAER M, THIRY M, GOESSENS G, 1993. Ultrastructural distribution of DNA within the ring shaped nucleolus of human resting T lymphocytes. Exp Cell Res 205: 430-432.
24. WACHTLER F, MOSGOELLER W, SCHWARZACHER HG, 1990. Electron microscopic in situ hybridisation and autoradiography: localisation and transcription of rDNA in human lymphocyte nucleoli. Exp Cell Res 198: 346-348.