Expression of the extracellular levansucrase and invertase genes from zymomonas mobilis in escherichia coli cells

Bioscience, Biotechnology and Biochemistry

Volumn 62, Issue 9, 1998, Pages 1802-1805

  Yanase, Hideshi ^a   Fujimoto, Junichi ^a   Maeda, Masahiro ^a   Okamoto, Kenji ^a   Kita, Keiko ^a   Tonomura, Kenzo ^a,b  

^a KAGOSHIMA UNIVERSITY   (Japan)

^b FUKUYAMA UNIVERSITY   (Japan)

Author keywords

Extracellular invertase; Extracellular levansucrase; Secretion; Signal peptide independent pathway; Zymomonas mobilis

Indexed keywords

BETA FRUCTOFURANOSIDASE; GLYCOSIDASE; GLYCOSYLTRANSFERASE; LEVANSUCRASE;

AMINO ACID SEQUENCE; ARTICLE; BACTERIAL GENE; ENZYMOLOGY; ESCHERICHIA COLI; GENE EXPRESSION; GENETICS; MOLECULAR GENETICS; ZYMOMONAS;

AMINO ACID SEQUENCE; BETA-FRUCTOFURANOSIDASE; ESCHERICHIA COLI; GENE EXPRESSION; GENES, BACTERIAL; GLYCOSIDE HYDROLASES; HEXOSYLTRANSFERASES; MOLECULAR SEQUENCE DATA; ZYMOMONAS;

EID: 0032151693     PISSN: 09168451     EISSN: 13476947     Source Type: Journal    
DOI: 10.1271/bbb.62.1802     Document Type: Article

References (21)

1. Yanase, H., Kato N., and Tonomura, K., “Strain improvement of Zymomonas mobilis for ethanol production/’ Recombinant Microbes for Industrial and Agricultural Applications. Ed. Y. Murooka and T. Imanaka. New York: Marcel Dekker, Inc., 723-739 (1994).
2. Brestic-Goachet, N., Gunasekaran, P., Cami, B., and Baratti, J.E., Transfer and expression of an Eriwinia chrysanthemi cellu- lase gene in Zymomonas mobilis. J. Gen. Microbiol., 135, 893-899 (1989).
3. Misawa, N., Okamoto, T., and Nakamura, K., The expression of a cellulase gene in Zymomonas mobilis. J. Biotechnol., 7, 167-178 (1988).
4. Yanase, H., Fukushi, H., Ueda, N., Maeda, Y., Toyoda, A., and Tonomura, T., Cloning, Sequencing, and Characterization of the intracellular invertase gene from Zymomonas mobilis. Agric. Biol. Chem., 55, 1383-1390 (1991).
5. Yanase, H., Iwata, I., Nakahigashi, R., Kita, K., Kato, N., and Tonomura, T., Purification, crystallization, and properties of the extracellular levansucrase from Zymomonas mobilis. Biosci. Biotech. Biochem., 56, 1335-1337 (1992).
6. Yanase, H., Iwata, M., Kita, K., Kato, N., and Tonomura, T., Purification, crystallization, and characterization of the extracellular invertase from Zymomonas mobilis. J. Ferment. Bioeng., 79, 367-369 (1995).
7. Kyono, K., Yanase, H., Tonomura, K., Kawasaki, H., and Sakai, T., Cloning and characterization of Zymomonas mobilis genes encoding extracellular levansucrase and invertase. Biosci. Biotech. Biochem., 59, 289-293 (1995).
8. Somogyi, M. J., Notes on sugar determination. J. Biol. Chem., 195, 19-23 (1952).
9. Anraku, Y. and Hepple, L. A., On the nature of the changes induced in Escherichia coli by osmotic shok. J. Biol. Chem., 242, 20) 2561-2569 (1967).
10. Ahluwalia, G. S. and Williams, H. H., Alkaline phosphatase activity from Escherichia coli grown on selenite media. Arch. Biochem. Biophys., 117, 192-193 (1966).
11. Steinmetz, M., Le Coq, D., Djemia, H. B., and Gay, P., Ana- lyse genetique de sacB, gene de structure d’une enzyme secretee, la levane-saccharase de Bacillus subtilis Marburg. Mol. Gen. Genet, 191,138-144 (1983).
12. Duggleby, R. G. and Dennis, D. T., Nicotinamide adenine dinucleotide-specific glyceraldehyde 3-phosphate dehydrogenase from Pisum sativum. Purification and characterization. J. Biol. Chem., 249, 162-166 (1974).
13. Gay, P., Le Coq, D., Steinmetz, M., Berkelman, T., and Kado, C. I., Positive selection procedure for entrapment of insertion sequence elements in a Gram-negative bacterium., J. Bacteriol., 164, 918-921 (1985).
14. Ried, J. L. and Collmer, A., An nptl-sacB-sacR cartridge for constructing directed, unmarked mutations in gram-negative bacteria by marker change-evidence mutagenesis. Gene, 57, 239-246 (1987).
15. Pugsley, A. P., The complete general secretory pathway in gram-negative bacteria. Microbiol. Rev., 57, 50-108 (1993).
16. Gusso, J., Murgier, M., Filloux, A., and Lazdunski, A., Cloning of the Pseudomonas aeruginosa alkaline protease gene and secretion of the protease into the medium by Escherichia coli. J. Bacteriol., 172, 942-948 (1990).
17. Delepelaire, P. and Wandersman, C., Proteases secretion by Er-winia chrysanthemi. Protease B and C are synthesized and secreted as zymogens without a signal peptide. J. Biol. Chem., 15, 9083-9089 (1989).
18. Akatsuka, H., Kawai, E., Omori, K. Komatsubara, S. Shibata, T. and Tosa, T., The lip A gene of Serratia marcescens which encodes an extracellular lipase having no A-terminal signal peptide. J. Bacteriol., 176, 1949-1956 (1994).
19. Mackman, N., Nicaud, J. M., Gray, Y., and Holland, I. B., Secretion of haemolysin by Escherichia coli. Curr. Top. Microbiol. Immunol., 125, 159-181 (1986).
20. Delepelaire, P. and Wandersman, C., Protein secretion in gram-negative bacteria. The extracellular metalloprotease B from Er-winia chrysanthemi a C-terminal secretion signal analogous to that of Escherichia coli alpha-haemolysin. J. Biol. Chem., 265, 17118-17125 (1990).
21. Sylvie, L., Delepelaire, P., and Wandersman, C., Cloning and expression in Escherichia coli of the Serratia marcescens metalloprotease gene: Secretion of the protease from E. coli in the presence of the Erwinia chrysanthemi protease secretion functions. J. Bacteriol., 173, 2160-2166 (1991).