Selective recovery of proteins by control of their surface properties utilizing PEG-bound affinity ligands

Journal of Chemical Engineering of Japan

Volumn 31, Issue 4, 1998, Pages 618-625

  Kuboi, Ryoichi ^a   Hasegawa, Tetsuhiro ^a   Yamahara, Katsuhito ^a   Komasawa, Isao ^a   Johansson, Göte ^b  

^a OSAKA UNIVERSITY   (Japan)

^b LUND UNIVERSITY   (Sweden)

Author keywords

Affinity Ligand; Aqueous Two Phase System; Extraction; Precipitation; Separation

Indexed keywords

ENZYMES; HYDROPHOBICITY; POLYETHYLENE GLYCOLS; PRECIPITATION (CHEMICAL); SOLUBILITY; SURFACE ACTIVE AGENTS; SURFACE PROPERTIES;

AFFINITY LIGANDS; AQUEOUS TOW-PHASE SYSTEMS (ATPS);

EXTRACTION;

PROTEIN; SEPARATION; TWO-PHASE FLOW;

EID: 0032147390     PISSN: 00219592     EISSN: None     Source Type: Journal    
DOI: 10.1252/jcej.31.618     Document Type: Article

References (17)

1. Albertsson, P. A.; Partition of Cell Particles and Macromolecules, 3rd ed., p. 112-146, John Wiley, New York, USA (1986)
2. Bordier, C.; "Phase Separation of Integral Membrane Proteins in Triton X-114 Solution," J. Biol. Chem., 256, 1604-1606 (1981)
3. Bradford, M. M.; "A Rapid and Sensitive Method for the Quantification of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye Binding," Anal. Biochem., 72, 248-254 (1976)
4. Brown, J. R. and P. Shockley; Lipid-Protein Interactions, p. 25-68, John Wiley, New York, USA (1982)
5. Cleland, J. L. and D. I. Wang; "Refolding and Aggregation of Bovine Carbonic Anhydrase B: Quasi-Elastic Light Scattering Analysis," Biochemistry, 29, 11072-11078 (1990)
6. Harrison, R. G.; Protein Purification Process Engineering, p. 115-208, Marcel Dekker, New York, USA (1994)
7. Horio, T.; Theory and Practice on Enzymes and other Proteins, 2nd ed, p. 93-117, Nankodo, Tokyo, Japan (1994)
8. Ingham, K. C.; "Precipitation of Proteins with Polyethylene Glycol," Methods in Enzymology, 182, 301-306 (1990)
9. Johansson, G. and M. Joelsson; "Preparation of Cibacron Blue F3G-A (Polyethylene Glycol) in Large Scale for Use in Affinity Partitioning," Biotechnol. Bioeng., 27, 621-625 (1985)
10. Johansson, G. and M. Joelsson; "Specifically Increased Solubility of Enzymes in Polyethyleneglycol Solutions Using Polymer-Bound Triazine Dyes," Anal. Biochem., 158, 104-110 (1986)
11. Johansson, G. and M. Joelsson; "Affinity Partitioning of Enzymes Using Dextran-Bound Procion Yellow HE-3G. Influence of Dye-Ligand Density," J. Chromatogr., 393, 195-208 (1987)
12. Johansson, G. and M. Joelsson; "Protein-Ligand Interactions Studied on Bovine Serum Albumin with Free and Polymer-Bound Cibacron Blue F3G-A as Ligand with Reference to Affinity Partitioning," J. Chromatogr., 537, 219-233 (1991)
13. Kuboi, R., K. Yano and I. Komasawa; "Evaluation of Surface Properties and Partitioning of Proteins in Aqueous Two-Phase Extraction Systems," Solv. Extr. Res. Dev. Japan, 1, 42-52 (1994)
14. Kuboi, R., T. Hasegawa, K. Yano and I. Komasawa; "Purification Process for Heat Shock Proteins Using Aqueous Two-Phase System and PEG Fractional Precipitation," J. Chem. Eng. Japan, 28, 797-802 (1995a)
15. Kuboi, R., H. Umakoshi, N. Takagi and I. Komasawa; "Optical Disruption Methods for the Selective Recovery of β-Galactosidase from Escherichia coli," J. Ferment, Bioeng., 79, 335-341 (1995b)
16. Shanbhag, V. P. and G. Johansson; "Specific Extraction of Human Serum Albumin by Partition in Aqueous Biphasic Systems Containing Poly(ethylene glycol)-bound Ligand," Biochem. Biophys. Res. Commun., 61, 1141-1146 (1974)
17. Yano, K., T. Hasegawa, R. Kuboi, I. Komasawa and T. Tsuchido; "Characterization of Surface Properties of Heat Shock Proteins for the Separation Using Aqueous Two-Phase Systems," J. Chem. Eng. Japan, 27, 808-814 (1994)