Inhibition of nonspecific binding of fluorescent-labelled antibodies to human eosinophils

Journal of Immunological Methods

Volumn 217, Issue 1-2, 1998, Pages 113-119

  Mahmudi Azer, S ^a   Lacy, P ^a   Bablitz, B ^a   Moqbel, R ^a  

^a UNIVERSITY OF ALBERTA   (Canada)

Author keywords

Blocking; BODIPY FL; FITC; Fluorochromes; IL 6; Immunofluorescence

Indexed keywords

FLUORESCEIN ISOTHIOCYANATE; FLUOROCHROME; IMMUNOGLOBULIN G; INTERLEUKIN 6; INTERLEUKIN 6 ANTIBODY; SERUM ALBUMIN;

ANTIBODY LABELING; ANTIBODY SPECIFICITY; ANTIGEN BINDING; ARTICLE; CHEMICAL BOND; CONCENTRATION RESPONSE; CONFOCAL LASER MICROSCOPY; CONTROLLED STUDY; CYTOKINE PRODUCTION; CYTOKINE RELEASE; EOSINOPHIL; FETAL CALF SERUM; GOAT; HORSE; HUMAN; IMMUNOFLUORESCENCE TEST; INHIBITION KINETICS; INTERMETHOD COMPARISON; MOUSE; NONHUMAN; PHOTOAFFINITY LABELING; PRIORITY JOURNAL; RAT; SERUM;

ANIMALS; ANTIGEN-ANTIBODY REACTIONS; ARTIFACTS; ASTHMA; BLOOD PROTEINS; BORON COMPOUNDS; CATTLE; CYTOPLASMIC GRANULES; EOSINOPHIL GRANULE PROTEINS; EOSINOPHILS; FALSE POSITIVE REACTIONS; FLUORESCEIN-5-ISOTHIOCYANATE; FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT; FLUORESCENT DYES; GOATS; HORSES; HUMANS; IMMUNOGLOBULIN G; INTERLEUKIN-6; MICROSCOPY, CONFOCAL; PROTEIN BINDING; RIBONUCLEASES; SERUM ALBUMIN, BOVINE; XANTHENES;

EID: 0032144232     PISSN: 00221759     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0022-1759(98)00105-7     Document Type: Article

References (25)

1. Ackerman S.J., Loegering D.A., Venge P., Olsson I., Harley J.B., Fauci A.S., Gleich G.J. Distinctive cationic proteins of the human eosinophil granule: major basic protein, eosinophil cationic protein, and eosinophil-derived neurotoxin. J. Immunol. 131:1983;2977.
2. Beutner E.H., Sepulveda M.R., Barnet E.V. Quantitative studies of immunofluorescent staining. Relationships of characteristics of unabsorbed antihuman IgG conjugates to their specific and nonspecific staining properties in an indirect test for antinuclear factors. Bull. W.H.O. 39:1968;587.
3. Detlefs R.L., Frieden I.J., Berger T.G., Westrom D. Eosinophil fluorescence: a cause of false positive slide tests for Herpes simplex virus. Ped. Dermatol. 4:1987;129.
4. Dubucquoi S.P., Desreumaux P., Janin A., Klein O., Goldman M., Tavernier J., Capron A., Capron M. Interleukin 5 synthesis by eosinophils: association with granules and immunoglobulin-dependent secretion. J. Exp. Med. 179:1994;703.
5. Egesten A., Calafat J., Knol E.F., Janssen H., Walz T.M. Subcellular localization of transforming growth factor-alpha in human eosinophil granulocytes. Blood. 87:1996;3910.
6. Filley W.V., Kephart G.M., Holley K.E., Gleich G.J. Identification by immunofluorescence of eosinophil granule major basic protein in lung tissues of patients with bronchial asthma. Lancet. 2:1982;11.
7. Floyd K., Suter P.F., Lutz H. Granules of blood eosinophils are stained directly by anti-immunoglobulin fluorescein isothiocyanate conjugates. Am. J. Vet. Res. 44:1983;2060.
8. Gleich G.J., Adolphson C.R., Lieferman K.M. The biology of the eosinophilic leukocyte. Annu. Rev. Med. 44:1993;85.
9. Johnston N.W., Bienenstock J. Abolition of nonspecific fluorescent staining of eosinophils. J. Immunol. Methods. 4:1974;189.
10. Kita H. The eosinophil: a cytokine-producing cell? J. Allergy Clin. Immunol. 97:1996;889.
11. Krenik K.D., Kephart G.M., Offord K.P., Dunnette S.L., Gleich G.J. Comparison of antifading agent used in immunofluorescence. J. Immunol. Methods. 117:1989;91.
12. Krug N., Thurau A.M., Lackie P., Baier J., Schultze-Werninghaus G., Rieger C.H., Schauer U. A flow cytometric method for the detection of intracellular basic proteins in unseparated peripheral blood and bone marrow eosinophils. J. Immunol. Methods. 190:1996;245.
13. Lacy P., Levi-Schaffer F., Mahmudi-Azer S., Bablitz B., Hagen S.C., Velazquez J., Kay A.B., Moqbel R. Intracellular localization of interleukin-6 in eosinophils from atopic asthmatics and effects of interferon-γ Blood. 91:1998;2508.
14. Levi-Schaffer F., Lacy P., Severs N.J., Newman T.M., North J., Gomperts B., Kay A.B., Moqbel R. Association of granulocyte/macrophage colony-stimulating factor with the crystalloid granule of human eosinophils. Blood. 85:1995;2579.
15. Levi-Schaffer F., Barkans J., Newman T.M., Ying S., Wakelin M., Hohenstein R., Barak V., Lacy P., Kay A.B., Moqbel R. Identification of interleukin-2 in human peripheral blood eosinophils. Immunology. 8:1996;155.
16. Moqbel R. Synthesis and storage of regulatory cytokines in human eosinophils. Adv. Exp. Med. Biol. 409:1996;287.
17. Moqbel R., Levi-Schaffer F., Kay A.B. Cytokine generation by eosinophils. J. Allergy Clin. Immunol. 94:1994;1183.
18. Moqbel R., Ying S., Barkans J., Newman T.M., Kimmitt P., Wakelin M., Taborda-Barata L., Meng Q., Corrigan C.J., Durham S.R., Kay A.B. Identification of messenger RNA for IL-4 in human eosinophils with granule localization and release of the translated product. J. Immunol. 155:1995;4939.
19. Nairn, R.C., 1969. Immunological tracing: general considerations. In: Fluorescent Protein Tracing, 3rd edn. E. & S. Livingstone, Edinburgh, p. 136.
20. Nairn R.C., Herzog F., Ward H.A., De Boer W.G. Microphotometry in immunofluorescence. Clin. Exp. Immunol. 4:1969;697.
21. Nonaka M., Nonaka R., Woolley K. Distinct immunohistochemical localization of IL-4 in human inflamed airway tissues. J. Immunol. 155:1995;3234.
22. Robenson J.L., Tilton R.D. Effect of fluorescence quenching on fluorescence recovery after photobleaching measurements. Biophys. J. 68:1995;2145.
23. Wardlaw A.J., Moqbel R., Kay A.B. Eosinophils: biology and role in disease. Adv. Immunol. 60:1995;151.
24. Weller P.F. Eosinophils: structure and functions. Curr. Opin. Immunol. 6:1994;85.
25. Wells, S., Johnson, I., 1994. Three-dimensional confocal microscopy. In: Steven, J.K., Mills, L.R., Trogadis, J.E. (Eds.), Investigation of Biological Specimens. Academic Press, San Diego, p. 101.