Isolation and characterization of two cytochrome P450 cDNA clones for CYP6B6 and CYP6B7 from Helicoverpa armigera (Hubner): Possible involvement of CYP6B7 in pyrethroid resistance

Insect Biochemistry and Molecular Biology

Volumn 28, Issue 8, 1998, Pages 571-580

  Ranasinghe, Charani ^a   Hobbs, Andrew A ^a  

^a UNIVERSITY OF WESTERN AUSTRALIA   (Australia)

Author keywords

PCR polymerase chain reaction; RACE, rapid amplification of cDNA ends; RT PCR, reverse transcription and polymerase chain reaction

Indexed keywords

COMPLEMENTARY DNA; CYTOCHROME 6B6; CYTOCHROME 6B7; CYTOCHROME P450; INSECTICIDE; PINENE; PYRETHROID; TERPENE; UNCLASSIFIED DRUG;

ANIMAL; ANIMAL CELL; ANIMAL TISSUE; ARTICLE; GENE CONVERSION; GENE SEQUENCE; GENETICS; HYBRIDIZATION; INSECT; INSECTICIDE RESISTANCE; LEPIDOPTERA; MOLECULAR CLONING; MOLECULAR GENETICS; NONHUMAN; NUCLEOTIDE SEQUENCE; PHYSIOLOGY; POLYMERASE CHAIN REACTION; PROTEIN STRUCTURE; SEQUENCE ANALYSIS; SEQUENCE HOMOLOGY;

ANIMALS; BASE SEQUENCE; CLONING, MOLECULAR; CYTOCHROME P-450 ENZYME SYSTEM; DNA, COMPLEMENTARY; INSECTICIDE RESISTANCE; INSECTICIDES; LEPIDOPTERA; MOLECULAR SEQUENCE DATA; PLANTS; POLYMERASE CHAIN REACTION; PYRETHRINS; SEQUENCE HOMOLOGY, AMINO ACID;

ANIMALIA; ARACHNIDA; HELICOVERPA ARMIGERA; HEXAPODA; INSECTA; INVERTEBRATA; LEPIDOPTERA;

EID: 0032143563     PISSN: 09651748     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0965-1748(98)00045-9     Document Type: Article

References (37)

1. Brattsten L.B., Holyoke J.C.W., Leeper J.R., Raffa K.F. Insecticide resistance: Challenge to pest management and basic research. Science. 231:1986;1255-1260.
2. Carino F.A., Koener J.F., Plapp F.W. Jr., Feyereisen R. Constitutive over-expression of the cytochrome P450 gene CYP6A1 in a house fly strain with metabolic resistance to insecticides. Insect Biochem. Mol. Biol. 24:1994;411-418.
3. Chomczynski P., Sacchi N. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal. Biochem. 162:1987;156-159.
4. Cohen M.B., Koener J.F., Feyereisen R. Structure and chromosomal localization of CYP6A1, a cytochrome P450-encoding gene from the house fly. Gene. 146:1994;267-272.
5. Cohen M.B., Feyereisen R. A cluster of cytochrome P450 genes of the CYP6 family in the house fly. DNA Cell Biol. 14:1995;73-82.
6. Corpet F. Multiple sequence alignment with hierarchical clustering. Nucl. Acids Res. 16:1988;10881-10890.
7. Daly J.C., Fisk J.H. Inheritance of metabolic resistance to the synthetic pyrethroids in Australian Helicoverpa armigera (Lepidoptera: Noctuidae). Bull. Entomol. Res. 82:1992;5-12.
8. Dunkov B.C., Rodriguez-arnaiz R., Pittendrigh B., ffrench-Constant R.H., Feyereisen R. Cytochrome P450 gene clusters in Drosophila melanogaster. Molec. Gen. Genet. 251:1996;290-297.
9. Feinberg A.P., Vogelstein B. A technique for radio-labelling DNA restriction endonuclease fragments to high specific activity. Anal. Biochem. 132:1983;6-13.
10. Forrester, N.W., Cahill, M., Bird, L.T., Layland, J.K., 1993. Management of pyrethroid and endosulfan resistance in Helicoverpa amigera (Lepidoptera: Noctuidae) in Australia. Aust. Bull. Ent. Res. Spec, sup. 1.
11. Fraenkel G.S. The raison d'etre of secondary plant substances. Science. 129:1959;1466-1470.
12. Frohman M.A., Dush M.K., Martin G.R. Rapid production of full-length cDNA from rare transcripts: Amplification using a single gene-specific oligonucleotide primer. Proc. Natl. Acad. Sci. USA. 85:1988;8998-9002.
13. Frolov M.V., Alatortsev V.E. Cluster of cytochrome P450 genes on the X chromosome of Drosophila melanogaster. DNA Cell Biol. 13:1994;663-668.
14. Gunning R.V., Easton C.S., Greenup L.R., Edge V.E. Pyrethroid resistance in Heliothis armigera (Hubner) (Lepidoptera: Noctuidae) in Australia. J. Econ. Entomol. 77:1984;1283-1287.
15. Heim M.H., Meyer U.A. Evolution of a highly polymorphic human cytochrome P450 gene cluster: CYP2D6. Genomics. 14:1992;49-58.
16. Higashi Y., Tanae A., Inoue H., Hiromasa T., Fujii-Kuriyama Y. Aberrant splicing and missense mutations cause steroid 21-hydroxylase P450 (21C) deficiency in humans: possible gene conversion products. Proc. Natl. Acad. Sci. USA. 85:1988;7486-7490.
17. Hodgson, E., 1985. Microsomal mono-oxygenases. In: Kerkut, G.A., Gilbert, L.I., (Eds.), Comprehensive insect physiology, Vol 11. Pergamon Press, Oxford, pp. 225-321.
18. Hodgson E., Rose R.L., Ryu D.Y., Falls G., Blake B.L., Levi P.E. Pesticide-metabolizing enzymes. Toxicol. Letters. 3:1995;73-81.
19. Hoque, M.R., 1984. Effect of different host plants and artificial diet on the tolerance levels of Heliothis armigera (Hubner) and H. punctigera (Wallengren) to various insecticides. M.Sc. thesis, University of Queensland, Australia.
20. Hung C., Holzmacher R., Connolloy E., Berenbaum M., Schuler M. Conserved promoter elements in the CYP6B gene family suggest common ancestry for cytochrome P450 monooxygenases mediating furanocoumarin detoxification. Proc. Natl. Acad. Sci. USA. 93:1996;12200-12205.
21. Liu N., Tomita T., Scott J.G. Allele-specific PCR reveals that CYP6D1 is on chromosome 1 in the house fly, Musca domestica. Experientia. 51:1995;164-167.
22. Maitra S., Dombrowski S.M., Waters L.C., Ganguly R. Three second chromosome-linked clustered cyp6 genes show differential constitutive and barbital-induced expression in DDT-resistant and susceptible strains of Drosophila melanogaster. Gene. 180:1996;165-171.
23. Matsunaga E., Umeno M., Gonzalez F.J. The rat P450 IID subfamily: Complete sequences of four closely linked genes and evidence that gene conversions maintained sequence homogeneity at the heme-binding region of the cytochrome P450 active site. J. Molec. Evol. 30:1990;155-169.
24. Nelson D.R., Koymans L., Kamataki T., Stegeman J.J., Feyereisen R., Waxman D.J., Waterman M.R., Gotoh O., Coon M.J., Estabrook R.W., Gunsalus I.C., Nebert D.W. P450 Superfamily - update on new sequences, gene mapping, accession numbers and nomenclature. Pharmacogenetics. 6:1996;1-42.
25. Pascoe L., Curnow K.M., Slutsker L., Connell J.M., Speiser P.W., New M.I., White P.C. Glucocorticoid-suppressible hyperaldosteronism results from hybrid genes created by unequal crossovers between CYP11B1 and CYP11B2. Proc. Natl. Acad. Sci. USA. 89:1992;8327-8331.
26. Porter T.D., Coon M.J. Cytochrome P450: Multiplicity of isoforms, substrates, and catalytic and regulatory mechanisms. J. Biol. Chem. 266:1991;13469-13472.
27. Ranasinghe C., Headlam M., Hobbs A.A. Induction of the mRNA for CYP6B2, a pyrethroid inducible cytochrome p450, in Helicoverpa armigera (Hubner) by dietary monoterpenes. Arch. Insect Biochem. and Physiol. 34:1997;99-109.
28. Ranasinghe, C., Campbell, B., Hobbs, A.A. Over-expression of cytochrome P450 CYP6B7 mRNA and pyrethroid resistance in Australian populations of Helicoverpa armigera (Hubner). Pestic. Sci. (submitted).
29. Riskallah M., Dauterman W.C., Hodgson E. Host plant induction of microsomal monooxygenase activity in relation to diazinon metabolism and toxicity in larvae of the tobacco budworm Heliothis virescens. Pestic. Physiol. and Biochem. 25:1986;233-247.
30. Savonet V., Maenhaut C., Miot F., Pirson I. Pitfalls in the use of several 'Housekeeping' genes as standards for quantitation of mRNA: The example of thyroid cells. Anal. Biochem. 247:1997;167-170.
31. Sambrook, J., Fritsch, E.F., Maniatis, T., 1989. Molecular Cloning: A Laboratory Manual, 2nd ed. Cold Spring Harbor Laboratory Press, New York.
32. Scott J.G. Cytochrome p450 monooxygenase-mediated resistance to insecticides. J. Pestic. Sci. 21:1996;241-245.
33. d mutants: A difference absorption spectral study. Biochim. Biophys. Acta. 995:1989;116-121.
34. Teakle, R.E., Jensen, J.M., 1985. Heliothis punctiger. In: Singh, P., Moore, R.F., (Eds.), Handbook of insect rearing, Vol. 2. Elsevier, Amsterdam, pp. 312-322.
35. Von Wachenfeldt, C., Johnson, E.F., 1995. Structure of eukaryotic cytochrome P450 enzymes. In: Ortiz de Montellano, P.R., (Ed.), Cytochrome P450: Structure, Mechanism and Biochemistry. New York, Plenum Press, pp. 183-223.
36. Wang X.-P., Hobbs A.A. Isolation and sequence analysis of a cDNA clone for a pyrethroid inducible cytochrome P450. Insect Biochem. Mol. Biol. 25:1995;1001-1009.
37. Waters L.C., Zelhof A.C., Shaw B.J., Chang L.-Y. Possible involvement of the long terminal repeat of transposable element 17.6 in regulating expression of an insecticide resistance-associated P450 gene in Drosophila. Proc. Natl. Acad. Sci. USA. 89:1992;4855-4859.