The glutamate transporter, GLT-1, is expressed in cultured hippocampal neurons

Neurochemistry International

Volumn 33, Issue 2, 1998, Pages 95-100

  Brooks Kayal, Amy R ^a   Munir, Muhammad ^a   Jin, Hong ^a   Robinson, Michael B ^a  

^a UNIVERSITY OF PENNSYLVANIA   (United States)

Author keywords

EAAC1; GLT 1; Glutamate transporters; Hippocampus; Immunocytochemistry; Single cell mRNA amplification

Indexed keywords

ANTIBODY; CARRIER PROTEIN; GLIAL FIBRILLARY ACIDIC PROTEIN; GLUTAMIC ACID; MESSENGER RNA; NEURON SPECIFIC ENOLASE;

ANIMAL CELL; ARTICLE; CELL CULTURE; CONTROLLED STUDY; EMBRYO; GLIA; HIPPOCAMPUS; IMMUNOCYTOCHEMISTRY; NERVE CELL; NEUROFILAMENT; NONHUMAN; PHENOTYPE; PRIORITY JOURNAL; PROTEIN EXPRESSION; RAT;

AMINO ACID TRANSPORT SYSTEM X-AG; ANIMALS; ATP-BINDING CASSETTE TRANSPORTERS; CELLS, CULTURED; EMBRYO; GENE EXPRESSION; GLIAL FIBRILLARY ACIDIC PROTEIN; HIPPOCAMPUS; IMMUNOHISTOCHEMISTRY; NEURONS; RATS; RNA, MESSENGER;

EID: 0032143556     PISSN: 01970186     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0197-0186(98)00018-7     Document Type: Article

References (26)

1. Arriza, J.L., Eliasof, S., Kavanaugh, M.P., Amara, S.G., 1997. Excitatory amino acid transporter 5, a retinal glutamate transporter coupled to a chloride conductance. Proceedings of the National Academy of Science U.S.A. 94, 4155-4160.
2. A Receptor Subunit mRNAs in Individual Hippocampal Neurons In Vivo and In Vitro. Journal of Neurochemistry 70, 1017-1028.
3. Buchhalter, J.R., Dichter, M.A., 1991. Electrophysiological comparison of pyramidal and stellate nonpyramidal neurons in dissociated cell culture of rat hippocampus. Brain Research Bulletin 26, 333-338.
4. Choi, D.W., 1992. Excitotoxic cell death. Journal of Neurobiology 23, 1261-1276.
5. Danbolt, N.C., 1994. The high affinity uptake system for excitatory amino acids in the brain. Prog. Neurobio. 44, 377-396.
6. +] coupled L-glutamate transporter purified from rat brain is located in glial cell processes. Neurosci. 51, 295-310.
7. Dhond, R.P., Ben, A., Mennerick, S., Danbolt, N.C., Rothstein, J.D., Isenberg, K.E., Zorumski, C.F., 1997. Neuronal expression of GLT-1 glutamate transporter in hippocampal cultures. Society for Neuroscience Abstracts 23, 1791.
8. Eberwine, J., Yeh, H., Miyashiro, K., Cao, Y., Nair, S., Finnel, R., Zettel, M., Coleman, P., 1992. Analysis of gene expression in single live neurons. Proceedings of the National Academy of Science U.S.A. 89, 3010-3014.
9. Furuta, A., Rothstein, J.D., Martin, J.L., 1997. Glutamate transporter protein subtypes are expressed differentially during rat central nervous system development. J. Neuroscience 17, 8363-8375.
10. Gegelashvili, G., Civenni, G., Racagni, G., Danbolt, N.C., Schousboe, I., Schousboe, A., 1996. Glutamate receptor agonists up-regulate glutamate transporter GLAST in astrocytes. NeuroReport 8, 261-265.
11. Gegelashvili, G., Schousboe, A., 1997. High affinity glutamate transporters: Regulation of expression and activity. Molecular Pharmacology 52, 6-15.
12. Jin, H., Price, M., Dichter, M.A., Yudkoff, M., Brooks-Kayal, A.R., 1997. Expression of GABAA Receptor α6 Subunit mRNA in Cultured Hippocampal Neurons. Society for Neuroscience Abstracts 23, 100.
13. Killisch, I., Dotti, C.G., Laurie, D.G., Luddens, H., Seeburg, P.H., 1991. Expression patterns of GABAA receptor subtypes in developing hippocampal neurons. Neuron 7, 927-936.
14. Lehre, K.P., Levy, L.M., Ottersen, O.P., Storm-Mathisen, J., Danbolt, N.C., 1995. Differential expression of two glial glutamate transporters in the rat brain: Quantitative and immunocytochemical observations. Journal of Neuroscience 15, 1835-1853.
15. +]coupled L-glutamate transporter purified from rat brains confirms glial cell localization. Federation of European Biochemical Societies 317, 79-84.
16. Martin, L.J., Brambrink, A.M., Lehmann, C., Potera-Cailliau, C., Koehler, R., Rothstein, J., Traystman, R.J., 1997. Hypoxia-ischemia causes abnormalities in glutamate transporters and death of astroglia and neurons in newborn striatum. Annals of Neurology 42, 335-348.
17. Northington, F.J., Traystman, R.J., Koehler, R.C., Rothstein, J.D., Martin, L.J., In Press. Regional and cellular expression of GLT1 and EAAC1 glutamate transporter proteins in ovine fetal brain. Neuroscience.
18. Robinson, M.B., Djali, S., Buchhalter, J.R., 1993. Inhibition of glutamate uptake with L-trans-pyrrolidine-2,4-dicarboxylate potentiates glutamate toxicity in primary hippocampal cultures. Journal of Neurochemistry 61, 2099-2103.
19. Robinson, M.B., Dowd, L.A., 1997. Heterogeneity and functional properties of subtypes of sodium-dependent glutamate transporters in the mammalian central nervous system. Advances in Pharmacology 37, 69-115.
20. Rothman, S., Cowan, W.M., 1981. A scanning electron microscope study of the in vitro development of dissociated hippocampal cells. Journal of Comparative Neurology 195, 141-155.
21. Rothstein, J.D., Martin, L., Levey, A.I., Dykes-Hoberg, M., Jin, L., Wu, D., Nash, N., Kuncl, R.W., 1994. Localization of neuronal and glial glutamate transporters. Neuron 13, 713-725.
22. +-dependent glutamate transporters by cAMP analogs and neurons. Molecular Pharmacology 53, 355-369.
23. Smith, K.E., Kessler, J.A., 1988. Non-neuronal cell-conditioned medium regulates muscarinic receptor expression in cultured sympathetic neurons. Journal of Neuroscience 8, 2406-2413.
24. Sutherland, M.L., Delaney, T.A., Noebels, J.L., 1996. Glutamate transporter mRNA expression in proliferative zones of the developing and adult murine CNS. Journal of Neuroscience 16, 2191-2207.
25. Torp, R., Danbolt, N.C., Babaie, E., Bjoras, M., Seeberg, E., Storm-Mathisen, J., Ottersen, O.P., 1994. Differential expression of two glial glutamate transporters in the rat brain: an in situ hybridization study. Eur. J. Neurosci. 6, 936-942.
26. Ullensvang, K., Lehre, K.P., Storm-Mathisen, J., Danbolt, N.C., 1997. Differential developmental expression of the two rat brain glutamate transporter proteins GLAST and GLT. Eur. J. Neurosci. 9, 1646-1655.