In vitro studies of cochlear excitation

Current Opinion in Neurobiology

Volumn 8, Issue 4, 1998, Pages 475-479

  Ulfendahl, Mats ^a   Flock, Åke ^a  

^a KAROLINSKA INSTITUTE   (Sweden)

Author keywords

[No Author keywords available]

Indexed keywords

ANIMAL TISSUE; CELL VIABILITY; COCHLEA; CORTI ORGAN; EXCITATION; HAIR CELL; HEARING; IN VITRO STUDY; MAMMAL; NONHUMAN; PRIORITY JOURNAL; REVIEW; STRUCTURE ACTIVITY RELATION; TEMPORAL BONE;

EID: 0032143422     PISSN: 09594388     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0959-4388(98)80034-2     Document Type: Article

References (30)

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22. Fridberger A, van Maarseveen JTPW, Scarfone E, Ulfendahl M, Flock B, Flock Å. Pressure-induced basilar membrane position shifts and stimulus-evoked potentials in the low-frequency regions of the guinea pig cochlea. of special interest Acta Physiol Scand. 161:1997;239-252 The authors used the in vitro temporal bone preparation to study how changing the position of the basilar membrane affects the cochlear microphonic and summating potentials. The induced movements of the hearing organ and the sensory hair bundles were visualised using laser confocal microscopy.
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24. 2+ concentrations and causes dynamic contractions of the hearing organ. Proc Natl Acad Sci USA. 95:1998;7127-7132 Using light and epifluorescence microscopy, the authors demonstrate that acoustic overstimulation of the intact hearing organ causes an increase in intracellular calcium, as previously shown using isolated cells [27]. In addition, a new dynamic hearing organ response is described for the first time: following overstimulation, there is a reversible 'contraction' of the organ, seen as a reduction of its width.
25. of outstanding interest Karavitaki KD, Mountain DC, Cody AR. Electrically evoked micromechanical movements from the apical turn of the gerbil cochlea. Lewis ER, Long GR, Lyon RF, Narins PM, Steele CR, Hecht-Poinar E. Diversity in Auditory Mechanics. 1997;392-398 World Scientific Publishing Co, Singapore, This papers describes how organ of Corti movements in response to electrical stimulation are recorded using video-microscopy. Interestingly, it is shown that the electrically evoked displacements of the sensory hair bundles of the inner and outer hair cells are in phase with each other for the tested frequencies (20-3000 Hz). This is in contradiction to previous reports (see e.g. [19]) suggesting that the phase of the inner hair cell bundle should lead the outer hair cell bundle phase at low frequencies.
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30. Flock Å, Scarfone E, Ulfendahl M. Vital staining of the intact hearing organ: visualization of cellular structure with confocal microscopy. of outstanding interest Neuroscience. 83:1998;215-228 This paper describes how laser confocal microscopy and different fluorescently labelled indicators were used to visualise the detailed structure of the unfixed, living hearing organ in vitro. With the proper orientation of the cochlea, unique images of sensory cells and nerve fibres can be obtained, resembling what has previously only been seen in sections of fixed and embedded tissue.