Detection of Prevotella intermedia in subgingival plaque of adult periodontitis patients by polymerase chain reaction

Journal of Periodontal Research

Volumn 33, Issue 6, 1998, Pages 369-376

  Riggio, M P ^a   Lennon, A ^a   Roy, K M ^a  

^a GLASGOW DENTAL HOSPITAL AND SCHOOL   (United Kingdom)

Author keywords

Adult periodontitis; Polymerase chain reaction; Prevotella intermedia; Subgingival plaque

Indexed keywords

BACTERIAL DNA; DIAGNOSTIC AGENT; PRIMER DNA; RESTRICTION ENDONUCLEASE; RNA 16S;

ADULT; AGAR GEL ELECTROPHORESIS; ARTICLE; BACTERIAL GENE; BASE PAIRING; CLASSIFICATION; FEMALE; GENETICS; HUMAN; ISOLATION AND PURIFICATION; MALE; MICROBIOLOGY; MIDDLE AGED; PERIODONTITIS; POLYMERASE CHAIN REACTION; PREVOTELLA; PREVOTELLA INTERMEDIA; SENSITIVITY AND SPECIFICITY; TOOTH PLAQUE;

ADULT; BASE PAIRING; DENTAL PLAQUE; DNA PRIMERS; DNA RESTRICTION ENZYMES; DNA, BACTERIAL; ELECTROPHORESIS, AGAR GEL; FEMALE; GENES, BACTERIAL; HUMANS; MALE; MIDDLE AGED; PERIODONTITIS; POLYMERASE CHAIN REACTION; PREVOTELLA; PREVOTELLA INTERMEDIA; RNA, RIBOSOMAL, 16S; SENSITIVITY AND SPECIFICITY;

EID: 0032132999     PISSN: 00223484     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1600-0765.1998.tb02212.x     Document Type: Article

References (31)

1. Loesche WJ, Syed SA, Laughon BE, Stoll J. The bacteriology of acute necrotizing ulcerative gingivitis. J Periodontol 1982; 53: 223-230.
2. Kornman KS, Loesche WJ. The subgingival microbial flora during pregnancy. J Periodont Res 1980; 15: 111-122.
3. Slots J, Bragd L, Wikstrom M, Dahlén G. The occurrence of Actinobacillus actinomycetemcomitans. Bacteroides gingivalis and Bacteroides intermedius in destructive periodontal disease in adults. J Clin Periodontol 1986; 13: 570-577.
4. Slots J, Listgarten MA. Bacteroides gingivalis, Bacteroides intermedius and Actinobacillus actinomycetemcomitans in human periodontal diseases. J Clin Periodontol 1988; 15: 85-93
5. Dahlén G, Manji F, Baelum V, Fejerskov O. Putative periodontopathogens in "diseased" and "non-diseased" persons exhibiting poor oral hygiene J Clin Periodontol 1992; 19. 35-42.
6. Könönen E. Pigmented Prevotella species in the periodontally healthy oral cavity. FEMS Immunol Med Microbiol 1993; 6: 201-206.
7. Dahlén, G, Wikström M, Renvert S, Gmür R, Guggenheim B. Biochemical and serological characterization of Bacteroides intermedius strains isolated from the deep periodontal pocket. J Clin Microbiol 1990; 28: 2269-2274.
8. Gharbia SE, Haapasalo M, Shah HN, et al Characterization of Prevotella intermedia and Prevotella nigrescens isolates from periodontic and endodontic infections. J Periodontol 1994; 65: 56-61.
9. van Winkelhoff AJ, Carlee AW, de Graaff J. Bacteroides endodontalis and other black-pigmented Bacteroides species in odontogenic abscesses. Infect Immun 1985; 49: 494-497.
10. Gmür R, Guggenheim B. Antigenic heterogeneity of Bacteroides intermedius as recognized by monoclonal antibodies. Infect Immun 1983; 42: 459-470.
11. Nakazawa F, Zambon JJ, Reynolds HS, Genco RJ. Serological studies of oral Bacteroides intermedius. Infect Immun 1988; 56: 1647-1651.
12. Shah HN, Gharbia SE. Biochemical and chemical studies on strains designated Prevotella intermedia and proposal of a new pigmented species, Prevotella nigrescens sp. nov. Int J Syst Bacteriol 1992; 42: 542-546.
13. Devine DA, Pearce MA, Gharbia SE, Shah HN, Dixon RA, Gmür R. Species-specificity of monoclonal antibodies recognising Prevotella intermedia and Prevotella nigrescens. FEMS Microbiol Lett 1994; 120: 99-104.
14. Teanpaisan R, Douglas CWI. Walsh TF. Characterisation of black-pigmented anaerobes isolated from diseased and healthy periodontal sites. J Periodont Res 1995: 30: 245-251.
15. Conrads G, Brauner A. Non-radioactively labelled DNA probes for the detection of periodontopathogenic Prevotella and Porphyromonas species. FEMS Immunol Med Microbiol 1993; 6: 115-120.
16. Dix K, Watanabe SM, McArdle S, et al. Species-specific oligodeoxynucleotide probes for the identification of periodontal bacteria. J Clin Microbiol 1990: 28: 319-323.
17. Mättö J, Saarela M, von Troil-Lindén B, et al. Distribution and genetic analysis of oral Prevotella intermedia and Prevotella nigrescens. Oral Microbiol Immunol 1996; 11: 96-102.
18. Shah HN, Gharbia SE, Scully C, Finegold SM. Oligonucleotide probes to the 16S ribosomal RNA: implications of sequence homology and secondary structure with particular reference to the oral species Prevotella intermedia and Prevotella nigrescens. Oral Dis 1995; 1: 32-36.
19. Milsom SE, Sprague SV, Dymock D, Weightman AJ, Wade WG. Rapid differentiation of Prevotella intermedia and P. nigrescens by 16S rDNA PCR-RFLP. J Med Microbiol 1996; 44: 41-43.
20. Ashimoto A, Flynn MJ, Slots J. Molecular genetic detection of Bacteroides heparinolyticus in adult periodontitis. Oral Microbiol Immunol 1995; 10: 284-287.
21. Goncharoff P, Figurski DH, Stevens RH, Fine DH. Identification of Actinobacillus actinomycetemcomitans: polymerase chain reaction amplification of lKtA-specific sequences. Oral Microbiol Immunol 1993; 8: 105-110.
22. Ashimoto A, Chen C, Bakker I, Slots J. Polymerase chain reaction detection of 8 putative periodontal pathogens in subgingival plaque of gingivitis and advanced periodontitis lesions. Oral Microbiol Immunol 1996; 11: 266-273.
23. Watanabe K., Frommel TO. Porphyromonas gingivalis, Actinobacillus actinomycetemcomitans and Treponema denticola detection in oral plaque samples using the polymerase chain reaction. J Clin Periodontol 1996; 23: 212-219.
24. Devereux J, Haeberli P, Smithies O. A comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Res 1984; 12: 387-395.
25. Savitt ED, Strzempko MN, Vaccaro KK, Peros WJ, French CK. Comparison of cultural methods and DNA probe analyses for the detection of Actinobacillus actinomycetemcomitans, Bacteroides gingivalis, and Bacteroides intermedius in subgingival plaque samples. J Periodontol 1988; 59: 431-438.
26. Söder P-Ö, Jin LJ, Söder B. DNA probe detection of periodontopathogens in advanced periodontitis. Scand J Dent Res 1993; 101: 363-370.
27. Fukushima H, Moroi H, Inoue J, et al. Phenotypic characteristics and DNA relatedness in Prevotella intermedia and similar organisms. Oral Microbiol Immunol 1992; 7: 60-64.
28. Conrads G, Mutters R, Fischer J, Brauner A, Lütticken R, Lampert F. PCR reaction and dot-blot hybridization to monitor the distribution of oral pathogens within plaque samples of periodontally healthy individuals. J Periodontol 1996; 67: 994-1003.
29. Guillot E, Mouton C. PCR-DNA probe assays for identification and detection of Prevotella intermedia sensu stricto and Prevotella nigrescens. J Clin Microbiol 1997; 35: 1876-1882.
30. Fujise O, Hamachi T, Hirofuji T, Maeda K. Colorimetric microtiter plate based assay for detection and quantification of amplified Actinobacillus actinomycetemcomitans DNA. Oral Microbiol Immunol 1995; 10: 372-377.
31. Riggio MP, MacFarlane TW, MacKenzie D, Lennon A, Smith AJ, Kinane D. Comparison of polymerase chain reaction and culture methods for detection of Actinobacillus actinomycetemcomitans and Porphyromonas gingivalis in subgingival plaque samples. J Periodont Res 1996; 31: 496-501.