Analysis of a variant surface glycoprotein gene expression site promoter of Trypanosoma brucei by remodelling the promoter region

Molecular and Biochemical Parasitology

Volumn 94, Issue 1, 1998, Pages 67-85

  Blundell, Patricia A ^a,b   Borst, Piet ^b  

^a UNIVERSITY OF GLASGOW   (United Kingdom)

^b NETHERLANDS CANCER INSTITUTE   (Netherlands)

Author keywords

Expression sites; Promoters; Transcription; Trypanosoma brucei

Indexed keywords

VARIANT SURFACE GLYCOPROTEIN;

ARTICLE; GENE EXPRESSION; NONHUMAN; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN EXPRESSION; TRYPANOSOMA BRUCEI;

ANIMALS; BLOTTING, SOUTHERN; CLONING, MOLECULAR; CONSERVED SEQUENCE; FLUORESCENT ANTIBODY TECHNIQUE; GENE REARRANGEMENT; MEMBRANE GLYCOPROTEINS; MICE; OPEN READING FRAMES; PROMOTER REGIONS (GENETICS); PROTOZOAN PROTEINS; RATS; TRANSCRIPTION, GENETIC; TRANSFECTION; TRYPANOSOMA BRUCEI BRUCEI;

EID: 0032128036     PISSN: 01666851     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0166-6851(98)00051-6     Document Type: Article

References (61)

1. [1] Cross GAM. Cellular and genetic aspects of antigenic variation in trypanosomes. Annu Rev Immunol 1990;8:83-110.
2. [2] Clayton C. Developmental regulation of nuclear gene expression in Trypanosoma brucei. Prog Nucleic Acid Res Mol Biol 1992;43:37-66.
3. [3] Borst P, Rudenko G. Antigenic variation in African trypanosomes. Science 1994;264:1872-3.
4. [4] Pays E, Vanhamme L, Berberof M. Genetic controls for the expression of surface antigens in African trypanosomes. Annu Rev Microbiol 1994;48:25-52.
5. [5] Borst P, Rudenko G, Taylor MC, et al. Antigenic variation in trypanosomes. Arch Med Res 1996;27:379-88.
6. [6] Cross GAM. Antigenic variation in trypanosomes: secrets surface slowly. Bioessays 1996;18:283-91.
7. [7] Cross GAM. Identification, purification and properties of clone-specific glycoprotein antigens constituting the surface coat of Trypanosoma brucei. Parasitology 1975;71:393-417.
8. [8] Cully DF, Ip HS, Cross GAM. Coordinate transcription of variant surface glycoprotein genes and an expression site associated gene family in Trypanosoma brucei. Cell 1985;42:173-82.
9. [9] Navarro M, Cross GAM. DNA rearrangements associated with multiple consecutive directed antigenic switches in Trypanosoma brucei. Mol Cell Biol 1996;16:3615-25.
10. [10] Aline RF, Myler PJ, Gobright E, Stuart KD. Early expression of a Trypanosoma brucei VSG gene duplicated from an incomplete basic copy. J Euk Microbiol 1994;41:71-8.
11. [11] Bernards A, Van der Ploeg LHT, Frasch ACC, et al. Activation of trypanosome surface glycoprotein genes involves a duplication-transposition leading to an altered 3' end. Cell 1981;27:497-505.
12. [12] Bernards A, Van der Ploeg LHT, Gibson WC, et al. Rapid change of the repertoire of variant surface glycoprotein genes in trypanosomes by gene duplication and deletion. J Mol Biol 1986;190:1-10.
13. [13] De Lange T, Kooter JM, Luirink J, Borst P. Transcription of a transposed trypanosome surface antigen gene starts upstream of the transposed segment. EMBO J 1985;4:3299-306.
14. [14] Laurent M, Pays E, Van Meirvenne N, Matthyssens G, Williams RO, Steinert M. DNA rearrangements linked to expression of a predominant surface antigen gene of trypanosomes. Nature 1983;302:263-6.
15. [15] Lu Y, Alarcon CM, Hall T, Reddy LV, Donelson JE. A strand bias occurs in point mutations associated with variant surface glycoprotein gene conversion in Trypanosoma rhodesiense. Mol Cell Biol 1994;14:3971-80.
16. [16] Myler PJ, Allison J, Agabian N, Stuart K. Antigenic variation in African trypanosomes by gene replacement or activation of alternate telomeres. Cell 1984;39:203-11.
17. [17] Myler P, Nelson RG, Agabian N, Stuart K. Two mechanisms of expression of a predominant variant antigen gene of Trypanosoma brucei. Nature 1984;309:282-4.
18. [18] Myler PJ, Aline RF, Scholler JK, Stuart KD. Multiple events associated with antigenic switching in Trypanosoma brucei. Mol Biochem Parasitol 1988;29:227-41.
19. [19] Pays E, Van Assel S, Laurent M, et al. Gene conversion as a mechanism for antigenic variation in trypanosomes. Cell 1983;34:371-81.
20. [20] Pays E, Delauw MF, Van Assel S, et al. Modifications of a Trypanosoma brucei antigen gene repertoire by different DNA recombinational mechanisms. Cell 1983;35:721-31.
21. [21] Pays E, Guyaux M, Aerts D, Van Meirvenne N, Steinert M. Telomeric reciprocal recombination as a possible mechanism for antigenic variation in trypanosomes. Nature 1985;316:562-4.
22. [22] Rudenko G, McCulloch R, Dirks-Mulder A, Borst P. Telomere exchange can be an important mechanism of variant surface glycoprotein gene switching in Trypanosoma brucei. Mol Biochem Parasitol 1996;80:65-75.
23. [23] Shea C, Glass DJ, Parangi S, Van der Ploeg LHT. VSG gene expression site switches in T. brucei. J Biol Chem 1986;261:5056-63.
24. [24] Blundell PA, Rudenko G, Borst P. Targeting of exogenous DNA into Trypanosoma brucei requires a high degree of homology between donor and target DNA. Mol Biochem Parasitol 1996;76:215-29.
25. [25] Van der Ploeg LHT, Smith CL, Polvere RI, Gottesdiener KM. Improved separation of chromosome-sized DNA from Trypanosoma brucei, stock 427-60. Nucleic Acids Res 1989;17:3217-27.
26. [26] Cross M, Taylor MC, Borst P. Frequent loss of the active site during VSG expression site switching in vitro in Trypanosoma brucei. Mol Cell Biol 1998;18:198-205.
27. [27] Gottesdiener K, Chung H-M, Brown SD, Lee MG-S, Van der Ploeg LHT. Characterization of VSG gene expression site promoters and promoter-associated DNA rearrangement events. Mol Cell Biol 1991;11:2467-80.
28. [28] Gottesdiener KM, Goriparthi L, Masucci JP, Van der Ploeg LHT. A proposed mechanism for promoter-associated DNA rearrangement events at a variant surface glycoprotein gene expression site. Mol Cell Biol 1992;12:4784-95.
29. [29] Pays E, Coquelet H, Tebabi P, et al. Trypanosoma brucei: constitutive activity of the VSG and procyclin gene promoters. EMBO J 1990;9:3145-51.
30. [30] Zomerdijk JCBM, Ouellette M, ten Asbroek ALMA, et al. The promoter for a variant surface glycoprotein gene expression site in Trypanosoma brucei. EMBO J 1990;9:2791-801.
31. [31] Zomerdijk JCBM, Kieft R, Duyndam M, Shiels PG, Borst P. Antigenic variation in Trypanosoma brucei: a telomeric expression site for variant-specific surface glycoprotein genes with novel features. Nucleic Acids Res 1991;19:1359-68.
32. [32] Jefferies D, Tebabi P, Pays E. Transient activity assays of the Trypanosoma brucei variant surface glycoprotein gene promoter: control of gene expression at the posttranscriptional level. Mol Cell Biol 1991;11:338-43.
33. [33] Pham VP, Qi CC, Gottesdiener KM. A detailed mutational analysis of the VSG gene expression site promoter. Mol Biochem Parasitol 1996;75:241-54.
34. [34] Vanhamme L, Pays A, Tebabi P, Alexandre S, Pays E. Specific binding of proteins to the noncoding strand of a crucial element of the variant surface glycoprotein, procyclin, and ribosomal promoters of Trypanosoma brucei. Mol Cell Biol 1995;15:5598-606.
35. [35] Horn D, Cross GAM. A developmentally regulated position effect at a telomeric locus in Trypanosoma brucei. Cell 1995;83:555-61.
36. [36] Rudenko G, Blundell PA, Taylor MC, Kieft R, Borst P. VSG gene expression site control in insect form Trypanosoma brucei. EMBO J 1994;13:5470-82.
37. [37] Urmenyi TP, Van der Ploeg LHT. PARP promoter-mediated activation of a VSG expression site promoter in insect form Trypanosoma brucei. Nucleic Acids Res 1995;23:1010-8.
38. [38] Zomerdijk JCBM, Kieft R, Borst P. Insertion of the promoter for a variant surface glycoprotein gene expression site in an RNA polymerase II transcription unit of procyclic Trypanosoma brucei. Mol Biochem Parasitol 1993;57:295-304.
39. [39] Rudenko G, Blundell PA, Dirks-Mulder A, Kieft R, Borst P. A ribosomal DNA promoter replacing the promoter of a telomeric variant surface glycoprotein gene expression site can be efficiently switched on and off in Trypanosoma brucei. Cell 1995;83:547-53.
40. [40] Zomerdijk JCBM, Kieft R, Shiels PG, Borst P. Alpha-amanitin-resistant transcription units in trypanosomes: a comparison of promoter sequences for a VSG gene expression site and for the ribosomal RNA genes. Nucleic Acids Res 1991;19:5153-8.
41. [41] Lodes MJ, Smiley BL, Stadnyk AW, Bennett JL, Myler PJ, Stuart K. Expression of a retroposon-like sequence upstream of the putative Trypanosoma brucei variant surface glycoprotein gene expression site promoter. Mol Cell Biol 1993;13:7036-44.
42. [42] Carruthers VB, Van der Ploeg LHT, Cross GAM. DNA-mediated transformation of bloodstream-form Trypanosoma brucei. Nucleic Acids Res 1993;21:2537-8.
43. [43] Hirumi H, Hirumi K. Continuous cultivation of Trypanosoma brucei bloodstream forms in a medium containing a low concentration of serum protein without feeder cell layers. J Parasitol 1989;75:985-9.
44. [44] Carruthers VB, Cross GAM. High-efficiency clonal growth of bloodstream-and procyclic Trypanosoma brucei on agarose plates. Proc Natl Acad Sci USA 1992;8:8818-21.
45. [45] Lanham SM, Godfrey DG. Isolation of salivarian trypanosomes from man and other mammals using DEAE-cellulose. Exp Parasitol 1970;28:521-34.
46. [46] McCulloch R, Rudenko G, Borst P. Gene conversions mediating antigenic variation in Trypanosoma brucei can occur in variant surface glycoprotein expression sites lacking 70-base-pair repeat sequences. Mol Cell Biol 1997;17:833-43.
47. [47] Gritz L, Davies J. Plasmid-encoded hygromycin B resistance: the sequence of hygromycin B phosphotransferase gene and its expression in Escherichia coli and Saccharomyces cerevisiae. Gene 1983;25:179-88.
48. [48] Ligtenberg MJL, Bitter W, Kieft R, et al. Reconstitution of a surface transferrin binding complex in insect form Trypanosoma brucei. EMBO J 1994;13:2565-73.
49. [49] White TC, Rudenko G, Borst P. Three small RNAs within the 10 kb trypanosome rRNA transcription unit are analogous to Domain VII of other eukaryotic 28S rRNAs. Nucleic Acids Res 1986;14:9471-89.
50. [50] Sambrook J, Fritsch EF, Maniatis T. Molecular Cloning: A Laboratory Manual. NY: Cold Spring Harbor Laboratory Press, 1989.
51. [51] Van der Ploeg LHT, Schwartz DC, Cantor CR, Borst P. Antigenic variation in Trypanosoma brucei analysed by electrophoretic separation of chromosome-sized DNA molecules. Cell 1984;37:77-84.
52. [52] Hug M, Carruthers VB, Hartmann C, Sherman DS, Cross GAM, Clayton C. A possible role for the 3′-untranslated region in developmental regulation in Trypanosoma brucei. Mol Biochem Parasitol 1993;61:87-96.
53. [53] Lee MG-S, Van der Ploeg LHT. Homologous recombination and stable transfection in the parasitic protozoan Trypanosoma brucei. Science 1990;250:1583-7.
54. [54] Ten Asbroek ALMA, Ouellette M, Borst P. Targeted insertion of the neomycin phosphotransferase gene into the tubulin gene cluster of Trypanosoma brucei. Nature 1990;348:174-5.
55. [55] Ten Asbroek ALMA, Mol CAAM, Kieft R, Borst P. Stable transformation of Trypanosoma brucei. Mol Biochem Parasitol 1993;59:133-42.
56. [56] Van Leeuwen F, Wijsman ER, Kieft R, van der Marel GA, van Boom JH, Borst P. Localisation of the modified base J in telomeric VSG gene expression sites. Genes Dev 1997;11:3232-41.
57. [57] Van Leeuwen F, Wijsman ER, Kuyl-Yeheskiely E, van der Marel GA, van Boom JH, Borst P. The telomeric GGGTTA repeats of Trypanosoma brucei contain the hypermodified base J in both strands. Nucleic Acids Res 1996;24:2476-82.
58. [58] Pays E, Coquelet H, Pays A, Tebabi P, Steinert M. Trypanosoma brucei: posttranscriptional control of the variable surface glycoprotein gene expression site. Mol Cell Biol 1989;9:4018-21.
59. [59] Pays E, Tebabi P, Pays A, et al. The genes and transcripts of an antigen gene expression site from T. brucei. Cell 1989;57:835-45.
60. [60] Borst P, Bitter W, Blundell PA, et al. Control of VSG gene expression sites in Trypanosoma brucei. Mol Biochem Parasitol 1998;91:67-76.
61. [61] Davies KP, Carruthers VB, Cross GAM. Manipulation of the vsg co-transposed region increases expression-site switching in Trypanosoma brucei. Mol Biochem Parasitol 1997;86:163-77.