Assessment of telomere length in hematopoietic interphase cells using in situ hybridization and digital fluorescence microscopy

Cytometry

Volumn 32, Issue 3, 1998, Pages 163-169

  De Pauw, E S D ^a   Verwoerd, N P ^a   Duinkerken, N ^a   Willemze, R ^a   Raap, A K ^a   Fibbe, W E ^a   Tanke, H J ^a  

^a LEIDEN UNIVERSITY MEDICAL CENTER   (Netherlands)

Author keywords

Digital fluorescence microscopy; Hematopoietic cells; Interphase cells; PNA FISH; Telomeric length

Indexed keywords

PEPTIDE NUCLEIC ACID;

ARTICLE; BONE MARROW CELL; CELL CULTURE; CHROMOSOME; CHROMOSOME SIZE; DNA SEQUENCE; EUKARYOTIC CELL; FETUS LIVER; FLUORESCENCE MICROSCOPY; HEMATOPOIETIC CELL; HUMAN; HUMAN CELL; IN SITU HYBRIDIZATION; METAPHASE CHROMOSOME; METHODOLOGY; NUCLEIC ACID PROBE; PRIORITY JOURNAL; SENESCENCE; SOUTHERN BLOTTING; TELOMERE; UMBILICAL CORD BLOOD;

ADULT; CELL NUCLEUS; CELLS, CULTURED; CHROMOSOMES, HUMAN; FETUS; HEMATOPOIETIC STEM CELLS; HUMANS; IN SITU HYBRIDIZATION, FLUORESCENCE; INTERPHASE; METAPHASE; MICROSCOPY, FLUORESCENCE; TELOMERE;

EUKARYOTA; GENETTA;

EID: 0032127323     PISSN: 01964763     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1097-0320(19980701)32:3<163::AID-CYTO1>3.0.CO;2-L     Document Type: Article

References (16)

1. Blackburn EH: Telomeres: No end in sight. Cell 77:621-623, 1994.
2. Bryan TM, Englezou A, Gupta J, Bacchetti S, Reddel RR: Telomere elongation in immortal human cells without detectable telomerase activity. EMBO J 14:4240-4248, 1995.
3. Counter CM, Gupta J, Harley CB, Leber B, Bacchetti S: Telomerase activity in normal leukocytes and in hematologic malignancies. Blood 85:2315-2320, 1995.
4. Ellenberger SL, Young IT: Measurement based depth-of-focus. In: Proceedings of the ASCI'96 2nd Annual Conference of the Advanced School for Computing and Imaging Lommel, Belgium, June 5-7, Kerckhoffs EJH, Sloot JFM, Tonino AM, Vossepoel AM (eds). ASCI, Delft, 1996, pp 218-223.
5. Greider CW, Blackburn EH: Identification of a specific telomere terminal transferase activity in Tetrahymena extracts. Cell 43:405-413, 1985.
6. Harley CB, Futcher AB, Greider CW: Telomeres shorten during ageing of human nbroblasts. Nature 345:458-460, 1990.
7. Kallioniemi A, Kallioniemi OP, Sudar D, Rutovitz D, Gray JW, Waldman F, Pinkel D: Comparative genomic hybridization for molecular cytogenetic analysis of solid tumors. Science 258:818-821, 1992.
8. De Lange T, Shiue L, Myers R, Cox DR, Naylor SL, Killery AM, Varmus HE: Structure and variability' of human chromosome ends. Mol Cell Biol 10:518-527, 1990.
9. Lennon GG, Lehrbach H: Hybridization analyses of arrayed cDNA libraries. Trends Genet 7:314-317, 1991.
10. Lansdorp PM, Verwoerd NP, van de Rijke FM, Dragowska V, Little M-T, Dirks RW, Raap AK, Tanke HJ: Heterogeneity in telomere length of human chromosomes. Hum Mol Genet 5:685-691, 1996.
11. Nederlof PM, van der Flier S, Verwoerd NP, Vrolijk J, Raap AK, Tanke HJ: Quantification of fluorescence in situ hybridization signals by image cytometry. Cytometry 13:846-852, 1992.
12. Nederlof PM, van der Flier S, Vrolijk J, Tanke HJ, Raap AK: Fluorescence ratio measurements of double-labeled probes for multiple in situ hybridization by digital microscopy. Cytometry 13:839-845, 1992.
13. Netten H, Young IT, van Vliet LJ, Tanke HJ, Vrolijk H, Sloos WCR: FISH and chips: Automation of fluorescent dot counting in interphase cell nuclei. Cytometry 28:1-10, 1997.
14. Slagboom PE, Droog S, Boomsma DI: Genetic determination of telomere size in humans: A twin study of three age groups. Am J Hum Genet 55:876-882, 1994.
15. Vaziri H, Dragowska W, Allsopp RC, Thomas TE, Harley CB, Lansdorp PM: Evidence for a mitotic clock in human hematopoietic stem cells: Loss of telomeric DNA with age. Proc Natl Acad Sci USA 91:9857-9860, 1994.
16. Watson JD: Origins of concatemeric T7DNA. Nat New Biol 239:197-201, 1972.