Purification of tubulin and microtubule-associated proteins by membrane ion-exchange chromatography

Protein Expression and Purification

Volumn 13, Issue 2, 1998, Pages 205-209

  Sloboda, Roger D ^a   Belfi, Lily M ^a  

^a Dartmouth College ^*  (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BUFFER; CELLULOSE PHOSPHATE; ION EXCHANGE CHROMATOGRAPHY; MICROTUBULE ASSOCIATED PROTEIN; TUBULIN;

VERTEBRATA;

EID: 0032127142     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1006/prep.1998.0902     Document Type: Article

References (10)

1. Sloboda R. D., Rudolph S. A., Rosenbaum J. L., Greengard P. Cyclic AMP-dependent endogenous phosphorylation of a microtubule-associated protein. Proc. Natl. Acad. Sci. USA. 72:1975;177-181.
2. Sloboda R. D., Dentler W. L., Rosenbaum J. L. Microtubule associated proteins and the stimulation of tubulin assembly in vitro. Biochemistry. 15:1976;4497-4505.
3. Weingarten M. D., Lockwood A. H., Hwo S-Y., Kirschner M. W. A protein factor essential for microtubule assembly. Proc. Natl. Acad. Sci. USA. 72:1975;1858-1862.
4. Sloboda R. D., Rosenbaum J. L. Purification and assay of microtubule associated proteins. Methods Enzymol. 85:1982;409-416.
5. Vallee R. B. Purification of brain microtubules and microtubule-associated protein 1 using Taxol. Methods Enzymol. 134:1986;104-115.
6. Sloboda R. D. Methods for the purification and assay of microtubule associated proteins. The Cytoskeleton: A Practical Approach. 1992;IRL Press at Oxford Univ. Press, London. p. 167-196.
7. Sloboda, R. D. 1998, The isolation and characterization of microtubules (tubulin and MAPs), in, Cells: A Laboratory Manual, Vol. 1, Culture and Biochemical Analysis of Cells, Spector, D. L.Goldman, R. D.Leinwand, L. A. 54.1, 54.22, Cold Spring Harbor Laboratory Press, New York.
8. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 227:1970;680-685.
9. Bradford M. M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72:1976;248-254.
10. Allen R. D., Allen N. S. Video-enhanced microscopy with a computer frame memory. J. Microsc. 129:1983;3-17.