Contrasting expression of KAL in cell-free systems: 5' UTR and coding region structural effects on translation

Protein Expression and Purification

Volumn 13, Issue 2, 1998, Pages 235-242

  De Zoysa, Priyal A ^a   Helliwell, Rachel J A ^a   Duke, Véronique M ^a   Quinton, Richard ^a   Bouloux, Pierre Marc G ^a  

^a ROYAL FREE HOSPITAL   (United Kingdom)

Author keywords

Anosmin 1; Cell free system; Coupled transcription translation; EMCV IRES; Firefly luciferase reporter; Nucleic acid conformation; X linked Kallmann's syndrome

Indexed keywords

COMPLEMENTARY DNA; ENCEPHALOMYOCARDITIS VIRUS; GENE EXPRESSION; TRANSCRIPTION REGULATION;

ENCEPHALOMYOCARDITIS VIRUS; ORYCTOLAGUS CUNICULUS;

EID: 0032126974     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1006/prep.1998.0891     Document Type: Article

References (32)

1. Schwanzel-Fukuda M., Bick D., Pfaff D. W. Luteinizing hormone-releasing hormone (LHRH)-expressing cells do not migrate normally in an inherited hypogonadal (Kallmann) syndrome. Mol. Brain Res. 6:1989;311-326.
2. Quinton R., Duke V. M., de Zoysa P. A., Bouloux P. G. The neurobiology of Kallmann's syndrome. Hum. Reprod. 11:1996;121-127.
3. Quinton R., Duke V. M., de Zoysa P. A., Platts A. D., Valentine A., Kendall B., Pickman S., Kirk J. M. W., Besser G. M., Jacobs H. S. The neuroradiology of Kallmann's syndrome: A genotypic and phenotypic analysis. J. Clin. Endocrinol. Metab. 81:1996;3010-3017.
4. Mayston M. J., Harrison L. M., Quinton R., Stephens J. A., Krams M., Bouloux P. M. G. Mirror movements in X-linked Kallmann's syndrome. 1. A neurophysiological study. Brain. 120:1997;1199-1216.
5. Krams M., Quinton R., Mayston M. J., Harrison L. M., Dolan R. J., Bouloux P. M. G., Stephens J. A., Frackowiak R. S. J., Passingham R. E. Mirror movements in X-linked Kallmann's syndrome. 2. A PET study. Brain. 120:1997;1217-1228.
6. Franco B., Guioli S., Pragliola A., Incerti B., Bardoni B., Tonlorenzi R., Carrozzo R., Maestrini E., Pieretti M., Taillon-Miller P. A gene deleted in Kallmann's syndrome shares homology with neural cell adhesion and axonal path finding molecules. Nature. 353:1991;529-535.
7. Legouis R., Hardelin J.-P., Levilliers J., Claverie J. M., Compain S., Wunderle V., Millasseau P., Le Paslier D., Cohen D., Caterina D. The candidate gene for the X-linked Kallmann syndrome encodes a protein related to adhesion molecules. Cell. 67:1991;423-435.
8. Incerti B., Guioli S., Pragliola A., Zanaria E., Borsani G., Tonlorenzi R., Franco B., Wheeler D., Ballabio A. Kallmann syndrome gene on the X and Y chromosomes: Implications for evolutionary divergence of human sex chromosomes. Nat. Genet. 2:1992;311-314.
9. del Castillo I., Cohen-Salmon M., Blanchard S., Lutfalla G., Petit C. Structure of the X-linked Kallmann syndrome gene and its homologous pseudogene on the Y chromosome. Nat. Genet. 2:1992;305-310.
10. Cohen-Salmon M., Tronche F., Del Castillo I., Petit C. Characterization of the promoter of the humanKAL. Gene. 164:1995;235.
11. Soussi-Yanicostas N., Hardelin J., del Mar Arroyo-Jimenez M., Ardouin O., Legouis R., Levilliers J., Traincard F., Betton J., Cabanie L., Petit C. Initial characterization of anosmin-1, a putative extracellular matrix protein synthesized by definite neuronal cell populations in the central nervous system. J. Cell Sci. 109:1996;1749-1757.
12. Rugarli E. I., Ghezzi C., Valsecchi V., Ballabio A. The Kallmann syndrome gene product expressed in COS cells cleaved on the cell surface to yield a diffusible component. Hum. Mol. Genet. 5:1996;1109-1115.
13. Duke V. M., Winyard P. J., Thorogood P., Soothill P., Bouloux P. M., Woolf A. S. KAL, Mol. Cell. Endocrinol. 110:1995;73-79.
14. Pelham H. R., Jackson R. J. An efficient mRNA-dependent translation system from reticulocyte lysates. Eur. J. Biochem. 67:1976;247-256.
15. Tymms M. J., Hertzog P. J. Structure-function studies based on in vitro expression. Methods Mol. Biol. 37:1995;317-335.
16. Takeshita T., Fujimori K., Shimizu N. Synthesis of biologically-active human nerve growth-factor and brain-derived neurotrophic factor by a cell free system. J. Ferment. Bioeng. 81:1996;13-17.
17. Sheffield W. P., Blajchman M. A. Deletion mutagenesis of heparin cofactor II: Defining the minimum size of a thrombin inhibiting serpin. FEBS Lett. 365:1995;189-192.
18. Rosenberg R. L., East J. E. Cell-free expression of functional Shaker potassium channels. Nature. 360:1992;166-169.
19. Craig D., Howell M. T., Gibbs C. L., Hunt T., Jackson R. J. Plasmid cDNA-directed protein synthesis in a coupled eukaryotic in vitro transcription-translation system. Nucleic Acids Res. 20:1992;4987-4995.
20. Bonner W. M., Laskey R. A. A film detection method for tritium-labelled proteins and nucleic acids in polyacrylamide gels. Eur. J. Biochem. 46:1974;83-88.
21. Borman A. M., Bailly J. L., Girard M., Kean K. M. Picornavirus internal ribosome entry segments: Comparison of translation efficiency and the requirements for optimal internal initiation of translation in vitro. Nucleic Acids Res. 23:1995;3656-3663.
22. Jackson R. J., Hunt S. L., Gibbs C. L., Kaminski A. Internal initiation of translation of picornavirus RNAs. Mol. Biol. Rep. 19:1994;147-159.
23. Parks G. D., Duke G. M., Palmenberg A. C. Encephalomyocarditis virus 3C protease: Efficient cell-free expression from clones which link viral 5′ noncoding sequences to the P3 region. J. Virol. 60:1986;376-384.
24. Duke G. M., Hoffman M. A., Palmenberg A. C. Sequence and structural elements that contribute to efficient encephalomyocarditis virus RNA translation. J. Virol. 66:1992;1602-1609.
25. Elroy-Stein O., Fuerst T. R., Moss B. Cap-independent translation of mRNA conferred by encephalomyocarditis virus 5′ sequence improves the performance of the vaccinia virus/bacteriophage T7 hybrid expression system. Proc. Natl. Acad. Sci. USA. 86:1989;6126-6130.
26. Kozak M. Influences of mRNA secondary structure on initiation by eukaryotic ribosomes. Proc. Natl. Acad. Sci. USA. 83:1986;2850-2854.
27. Kozak M. Point mutations define a sequence flanking the AUG initiator codon that modulates translation by eukaryotic ribosomes. Cell. 44:1986;283-292.
28. Zuker M. On finding all suboptimal foldings of an RNA molecule. Science. 244:1989;48-52.
29. Genetics Computer Group, 1994, Program Manual for the Wisconsin Package, Version 8, Genetics Computer Group, 575 Science Drive, Madison, WI, 53711.
30. Frances V., Morle F., Godet J. Identification of two critical base pairings in 5′ untranslated regions affecting translation efficiency of synthetic uncapped globin mRNAs. Biochim. Biophys. Acta. 1130:1992;29-37.
31. McCarthy J. E., Kollums H. Cytoplasmic mRNA-protein interactions in eukaryotic gene expression. Trends Biochem. Sci. 20:1995;191-197.
32. Verdi J. M., Campagnoni A. T. Translational regulation by steroids. Identification of a steroid modulatory element in the 5′-untranslated region of the myelin basic protein messenger RNA. J. Biol. Chem. 265:1990;20314-20320.