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The value of the baseline should be zero, since no fluorescence is occurring during this stage. However, the lens employed in the optical train depicted in Fig. 2 is too small in diameter to collimate all of the light emitted from the fiber optic. Light impinging the bandpass interference filter non-normally is not properly filtered, and therefore is partially passed through the filter. A larger diameter lens or an altered optical setup would eliminate this artifact.
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12
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0005428622
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