Purification and Characterization of a Protease from Actinobacillus pleuropneumoniae Serotype 1, An Antigen Common to All the Serotypes

Canadian Journal of Veterinary Research

Volumn 62, Issue 3, 1998, Pages 183-190

  Negrete Abascal, Erasmo ^a   Tenorio, Victor R ^b   Guerrero, Alma L ^a   García, Rosa M ^a   Reyes, Magda E ^a   De la Garza, Mireya ^a  

^a INSTITUTO POLITÉCNICO NACIONAL   (Mexico)

^b INSTITUTO NACIONAL DE INVESTIGACIONES FORESTALES   (Mexico)

Author keywords

[No Author keywords available]

Indexed keywords

IMMUNOGLOBULIN A; IMMUNOGLOBULIN G; PROTEINASE;

ACTINOBACILLUS PLEUROPNEUMONIAE; AFFINITY CHROMATOGRAPHY; ANIMAL; ARTICLE; CHEMISTRY; CLASSIFICATION; ENZYME SPECIFICITY; ENZYMOLOGY; FREEZE DRYING; ION EXCHANGE CHROMATOGRAPHY; ISOLATION AND PURIFICATION; KINETICS; METABOLISM; MOLECULAR WEIGHT; POLYACRYLAMIDE GEL ELECTROPHORESIS; SEROTYPING; SWINE; ULTRAFILTRATION;

ACTINOBACILLUS PLEUROPNEUMONIAE; ANIMALS; CHROMATOGRAPHY, AFFINITY; CHROMATOGRAPHY, DEAE-CELLULOSE; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ENDOPEPTIDASES; FREEZE DRYING; IMMUNOGLOBULIN A; IMMUNOGLOBULIN G; KINETICS; MOLECULAR WEIGHT; SEROTYPING; SUBSTRATE SPECIFICITY; SWINE; ULTRAFILTRATION;

EID: 0032110356     PISSN: 08309000     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (44)

1. BOHACH GA, SNYDER IS. Composition of atfinity-purified alpha-hemolysin of Esflierichia coli. Infect Immun 1986; 53: 435-437.
2. BOURGEAU G, LAPOINTE H, PÉLOQUIN P, MAYRAND D. Cloning, expression, and sequencing of a protease gene (tpr) from Porphyromonas gingivalis W83 in Escherichia colt. Infect Immun 1992; 60: 3186-3192.
3. BRADFORD MM. A rapid and sensible method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 1976; 72: 248-254.
4. CHEVALIER G, DUCLOHIER H, THOMAS D, SHECHTER E, WRÓBLEWSKI H. Purification and characterization of protein H, the major porin of Pasteurella multocida. J Bacteriol 1993; 175: 266-276.
5. COOLBEAR T, WHITTAKER JM, DANIEL RM. The effect of metal ions on the activity and thermostability of the extracellular proteinase from a thermophilic Bacillus, strain EA.1. Biochem J 1992; 287: 367-374.
6. CRUZ WT, NEDIALKOV YA, THACKER BJ, MULKS MH. Molecular characterization of a 48-kilodalton outer membrane protein of Actinobacillus pleuropneumoniae. Infect Immun 1996; 64: 83-90.
7. FEDORKA-CRAY PJ, STINE DL, GREENWALD JM, GRAY JT, HUETHER MJ, ANDERSON GA. The importance of secreted virulence factors in Actinobacillus pleuropneumoniae bacterin preparation: A comparison. Vet Microbiol 1993; 37: 85-100.
8. FENWICK BW, OSBURN BI. Immune responses to the lipopolysaccharides and capsular polysaccharides of Haemophilus pleuropneumoniae in convalescent and immunized pigs. Infect Immun 1986; 54: 575-582.
9. FINKELSTEIN RA, BOESMANFINKESTEIN, CHANG Y, HASE CC. Vibrio cholerae hemagglutinin/protease, colonial variation, virulence and detachment. Infect Immun 1992; 60: 472-478.
10. FREY J. Virulence in Actinobacillus pleuropneumaniae and RTX toxins. Trends Microbiol 1995; 3: 257-261.
11. GALLOWAY DR. Pseudomonas aeruginosa elastase and elastolysis revisited: recent developments. Mol Microbiol 1991; 5: 2315-2321.
12. GRIFFITH OM. Techniques of preparative, zonal, and continuous flow ultracentrifugation. Beckman Instruments, Inc. 1986.
13. HAGA Y, OGINO S, OHASHI S, AJITO T, HASHIMOTO K, SAWADA T. Protective efficacy of an affinity-purified hemolysin against experimental swine pleuropneumoma J Vet Med Sci 1997; 59: 115-120.
14. HASE CC, FINKELSTEIN RA. Bacterial extracellular zinc-containing metalloproteases. Microbiol Rev 1993; 57: 823-837.
15. HEUSSEN C, DOWDLE EB. Electrophoretic analysis of plasminogen activators in polyacrylamide gels containing sodium dodecyl sulfate and copolymerized substrates. Anal Biochem 1980; 102: 196-202.
16. IDRIS UEA, HARMON BG, UDEZE FA, KADIS S. Pulmonary lesions in mice inoculated with Actinobacillus pleuropneumoniae hemolysin and lipopolysaccharide. Vet Pathol 1993; 30: 234-241.
17. INZANA TJ, MA J, WORKMAN T, GOLOLEWSKI RP, ANDERSON P. Virulence properties and protective efficacy of the capsular polymer of Haemophilius (Actinobacillus) pleuropngumoniae serotype 5. Infect Immun 1988; 56: 1880-1889.
18. INZANA TJ, TODD J, MA J, VEIT H. Characterization of a non-hemolytic mutant of Actinobacillus pleuropneumoniae serotype 5: Role of the 110 kilodalton hemolysin in virulence and immunoprotection. Microbial Pathogen 1991; 10: 281-296.
19. KILIAN M, REINHOLD J, LOMHOLT H, POULSEN K, FRANDSEN EVG. Biological significance of IgA1 proteases in bacterial colonization and pathogenesis: Critical evaluation of experimental evidence. APMIS 1996; 104: 321-338.
20. KILIAN M, MESTECKY J, SCHROHENLOHER RE. Pathogenic species of the genus Haemophilus and Streptococcus pneumoniae produce immunoglobulin A1 protease. Infect Immun 1979; 26: 143-149.
21. McDERMIT AS, McKEE AS, MARSH PD. Effect of enviromental pH on enzyme activity and growth of Bacteroides gingivalis w50. Infect Immun 1998; 56: 1096-1100.
22. McKEVITT AI, BAJAKSOUZIAN S, KLINGER JD, WOODS DE. Purification and characterization of an extracellular protease from Pseudomonas cepacia. Infect Immun 1989; 57: 771-778.
23. MARTIN RG, AMES BN. A method for determining the sedimentation behavior of enzymes: Application to protein mixtures. J Biol Chem 1961; 236: 1372-1379.
24. NEGRETE-ABASCAL E, TENORIO VR, SERRANO JJ, GARCIA CC, DE LA GARZA M. Secreted proteases from Actinobacillus pleuropneumoniae serotype 1 degrade porcine gelatin, hemoglobin and immunoslobulin A. Can J Vet Res 1994; 58: 83-86.
25. NORQUIST A, NORMAN B, WOLFWATZ H. Identification and characterization of a zinc metalloprotease associated with invasion by the fish pathogen Vibrio anguiltlarum. Infect Immun 1990; 58: 3731-3736.
26. OISHI E, KITAJIMA T, KOYAMA Y, OHGITANI T, KATAYAMA S, OKABE T. Protective effect of the combined vaccine prepared from cell-free-antigen of Actinobacillus pleuropneumoniae serotypes 1, 2 and 5 in pigs. J Vet Med Sci 1995; 57: 1125-1128.
27. PACAUD M. Purification and characterization of two novel proteolytic enzymes in membrane of Escherichia coli. Protease IV and V. J Biol Chem 1982; 257: 4333-4339.
28. PARK KB, LABBE RG. Isolation and characterization of extracellular proteases of Clostridium perfringens type A. Curr Microbiol 1991; 23: 215-219.
29. PLAUT AG. Microbial IgA proteases. N Engl J Med 1978; 298: 1459-1463.
30. POTEMPA J, PIKE R, TRAVIS J. The multiple forms of trypsin-like-activity present in various strains of Porphyromonas gingivalis are due to the presence of either Arg-gingipain or Lys-gingipain. Infect Immun 1995; 63: 1176-1182.
31. QUINN FD, TOMPKINS LS. Analysis of a cloned sequence of Legionella pneumophila encoding a 38 kDa metalloprotease possessing haemolytic and cytotoxic activities. Mol Microbiol 1989; 3: 797-805.
32. ROSENDAL S, BOYD DA, GILBRIDE KA. Comparative virulence of porcine Haemophilus bacteria. Can I Comp Med 1985; 49: 68-74.
33. RYCROFT AN, WILLIAMS D, CULLEN JM, MACDONALD J. The cytotoxin of Actinobacillus pleuropneumoniae (pleurotoxin) is distinct from the haemolysin and is associated with a 120 kDa polypeptide. J Gen Microbiol 1991; 137: 561-568.
34. SEBUNYA TN, SAUNDERS JR. Haemophilus pleuropneumoniae infection in swine: A review. J Am Vet Med Assoc 1983; 182: 1331-1337.
35. SOJAR HT, LEE JY, BEDI GS, GENCO RJ. Purification and characterization of a protease from Porphyromonas gingivalis capable of degrading salt-solubilized collagen. Infect Immun 1993; 61: 2369-2376.
36. TENORIO V, FALCON A, CIPRIAN A, CAMACHO J. Pathogenicity of Haemophilus pleuropneumoniae in laboratory animals. 2nd Latino American Congress of the Pig Specialist Veterinary Association, Mexico. 1987: 179-181.
37. TEUFEL P, GÖTZ F. Characterization of an extracellular metalioprotease with elastase activity from Staphylococcus epidermidis J Bacteriol 1993; 175: 4218-4224.
38. TOWBIN H, STAEHELIN T, GORDON J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: Procedure and some applications. Proc Natl Acad Sci USA 1979; 76: 4350-4354.
39. TRAVIS J, POTEMPA J, MAEDA H. Are bacterial proteinases pathogenic factors? Trends Microbiol 1995; 3: 405-407.
40. UDEZE FA, LATIMER KS, KADIS S. Role of Haemophilus pleuropneumoniae lipopolysaccharide endotoxin in the pathogenesis of porcine Haemophilus pleuropneumonia. Am J Vet Res 1987; 48: 768-773.
41. UITTO V, GRENIER D, CHAN ECS, McBRIDE BC. Isolation of a chymotrypsin-like enzyme from Treponema denticola. Infect Immun 1988; 56: 2717-2722.
42. UTRERA V, PIJOAN C. Fimbriae in Actinobacillus pleuropneumoniae strains isolated from pig respiratory tracts. Vet Record 1991; 128: 357-358.
43. WASYLEWSKI Z, STRYJEWSKI W, WASNIOWSKA A, POTEMPA J, BARAN K. Effect of calcium binding on conformational changes of staphylococcal metalloproteinase measured by means of intrinsic protein fluorescence. Biochem Biophys Acta 1986; 871: 177-181
44. WESSEL D, FLÜGGE UI. A method for the quantitative recovery of protein in dilute solution in the presence of detergents and lipids. Anal Biochem 1984; 138: 141-143.