Down-regulation of specific members of the glutamine synthetase gene family in alfalfa by antisense RNA technology

Plant Molecular Biology

Volumn 37, Issue 3, 1998, Pages 535-547

  Temple, Stephen J ^a,b   Bagga, Suman ^a   Sengupta Gopalan, Champa ^a  

^a NEW MEXICO STATE UNIVERSITY   (United States)

^b UNIVERSITY OF MINNESOTA   (United States)

Author keywords

Alfalfa; Antisense; Gene family; Glutamine synthetase

Indexed keywords

ALFALFA; ANTISENSE RNA; ANTISENSE TECHNOLOGY; CHLOROPLAST; GENE EXPRESSION; GLUTAMATE AMMONIA LIGASE; TRANSCRIPTION REGULATION; TRANSGENIC PLANT;

DNA, RECOMBINANT; DOWN-REGULATION; GENE EXPRESSION REGULATION, ENZYMOLOGIC; GENE EXPRESSION REGULATION, PLANT; GLUTAMATE-AMMONIA LIGASE; MEDICAGO SATIVA; NUCLEIC ACID HYBRIDIZATION; RNA, ANTISENSE; RNA, MESSENGER; TRANSFORMATION, GENETIC;

EID: 0032104850     PISSN: 01674412     EISSN: None     Source Type: Journal    
DOI: 10.1023/A:1006099512706     Document Type: Article

References (49)

1. Austin S, Bingham ET, Mathews DE, Shahan MN, Will J, Burgess RR: Production and field performance of transgenic alfalfa (Medicago saliva L.) expressing alpha-amylase and manganese-dependent lignin peroxidase. Euphytica 85: 381-393 (1995).
2. Bagga S, Sutton D, Kemp JD, Sengupta-Gopalan C: Constitutive expression of the β-phaseolin gene in different tissues of transgenic alfalfa does not ensure phaseolin accumulation in non-seed tissue. Plant Mol Biol 19: 951-958 (1992).
3. Benjamin PM, Wu J, Mitchell AP, Magasanik B: Three regulatory systems control expression of glutamine synthetase in Saccharomyces cerevisiae at the level of transcription. Mol Gen Genet. 217: 370-377 (1989).
4. Bennett MJ, Lightfoot DA, Cullimore JV: cDNA sequences and differential expression of the gene encoding the glutamine synthetase γ polypeptide of Phaseolus vulgaris L. Plant Mol Biol 12: 553-565 (1989).
5. Blackwell RD, Murray AJS, Lea PJ: Inhibition of photosynthesis in barley with decreased levels of chloroplastic glutamine synthetase activity. J Exp Bot 38: 1799-1809 (1987).
6. Blokland RV, van de Geest N, Mol JNM, Kooter JM: Transgene-mediated suppression of chalcone synthase expression in Petunia hybrida results from an increase in RNA turnover. Plant J 6: 861-877 (1994).
7. Bourque JE: Antisense strategies for genetic manipulations in plants. Plant Sci 105: 125-149 (1995).
8. Cannon M, Platz J, O'Leary M, Sookdeo C, Cannon F: Organspecific modulation of gene expression in transgenic plants using antisense RNA. Plant Mol Biol 15: 39-47 (1990).
9. Cock JM, Brock IW, Watson AT, Swarup R, Morby AP, Cullimore JV: Regulation of glutamine synthetase genes in leaves of Phaseolus vulgaris. Plant Mol Biol 17: 761-771 (1991).
10. Cock JM, Hermon P, Cullimore JV: Characterization of the gene encoding the plastid-located glutamine synthetase of Phaseolus vulgaris: regulation of β-glucuronidase gene fusions in transgenic tobacco. Plant Mol Biol 18: 1141-1149 (1992).
11. Cornelissen M, Vandewiele M: Both RNA level and translation efficiency are reduced by anti-sense RNA in transgenic tobacco. Nucl Acids Res 17: 833-843 (1989).
12. Crawford NM: Nitrate: nutrient and signal for plant growth. Plant Cell 7: 859-868 (1995).
13. Cullimore JV, Miflin BJ: Immunological studies on glutamine synthetase using antisera raised to the two plant forms of the enzyme from Phaseolus root nodules. J Exp Bot 35: 581-587 (1984).
14. DasSarma S, Tischer E, Goodman HM: Plant glutamine synthase complements a glnA mutant in Escherichia coli. Science 232: 1242-1244 (1986).
15. Delauney AJ, Tabaeizadeh Z, Verma DPS: A stable bifunctional antisense transcript inhibiting gene expression in transgenic plants. Proc Natl Acad Sci USA 85: 4300-4304 (1988).
16. De Vries SC, Springer J, Wessels JGH: Diversity of abundant mRNA sequences and patterns of protein synthesis in etiolated and greened pea seedlings. Planta 156: 129-135 (1982).
17. Dubois F, Brugiere N, Sangwan RS, Hirel B: Localization of tobacco cytosolic glutamine synthetase enzymes and the corresponding transcripts shows organ- and cell-specific patterns of protein synthesis and gene expression. Plant Mol Biol 31: 807-817 (1996).
18. Edwards JW, Coruzzi GM: Photorespiration and light act in concert to regulate the expression of the nuclear gene for chloroplast glutamine synthetase. Plant Cell 1: 241-248 (1989).
19. Edwards JW, Walker EL, Coruzzi GM: Cell-specific expression in transgenic plants reveals nonoverlapping roles for chloroplast and cytosolic glutamine synthetase. Proc Natl Acad Sci USA 87: 3459-3463 (1990).
20. Fulks RM, Stadtman ER: Regulation of glutamine synthetase, aspartokinase and total protein turnover in Klebsiella arogenes. Biochim Biophys Acta 843: 214-229 (1985).
21. Hirel B, Bouet C, King B, Layzell D, Jacobs F, Verma DPS: Glutamine synthetase genes are regulated by ammonia provided externally or by symbiotic nitrogen fixation. EMBO J 6: 1167-1171 (1987).
22. Hirel B, Miao G-H, Verma DPS: Metabolic and developmental control of glutamine synthetase genes in legume and nonlegume plants. In: Verma DPS (ed) Control of Plant Gene Expression, pp. 443-458. CRC Press, Boca Raton, FL (1993).
23. Morsch RB, Fry FE, Hoffmann NL, Eichholtz D, Rogers SG, Fraley RT: A simple and general method for transferring genes into plants. Science 227: 1229-1231 (1985).
24. Jiang CZ, Kliebenstein D, Ke N, Rodermel S: Destabilization of rbcS sense transcripts by antisense RNA. Plant Mol Biol 25: 569-576 (1994).
25. Kim WT, Yang SF: Turnover of 1-aminocyclopropane-1-carboxylic acid synthase protein in wounded tomato fruit tissue. Plant Physiol 100: 1126-1131 (1992).
26. Kozaki A, Sakamoto A, Tanaka K, Takeba G: The promoter of the gene for glutamine synthetase from rice shows organ-specific and substrate-induced expression in transgenic tobacco plants. Plant Cell Physiol 32: 353-358 (1991).
27. Laemmli UK: Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 27: 680-685 (1990).
28. Lauerer M, Saftic D, Quick WP, Labate C, Fichtner K, Schulze E-D, Rodermel SR, Bogorad L, Stitt M: Decreased ribulose-1,5-bisphosphate carboxylase-oxygenase in transgenic tobacco transformed with antisense rbcS. Planta 190: 332-345 (1993).
29. Lea PJ, Robinson SA, Stewart GR: The enzymology and metabolism of glutamine, glutamate and asparagine. In: Miflin BJ, Lea PJ (eds) The Biochemistry of Plants, vol. 16. Intermediary Nitrogen Metabolism, pp. 121-159. Academic press, New York (1990).
30. Meister A: Glutamine synthetase. In: Mora J, Palacios R (eds) Glutamine: Metabolism, Enzymology and Regulation, pp. 1-40. Academic Press, New York (1980).
31. Miao G-H, Hirel B, Marsolier MC, Redge RW, Verma DPS: Ammonia-regulated expression of a soybean gene encoding cytosolic glutamine synthetase in transgenic Lotus corniculatus. Plant Cell 3: 11-22 (1991).
32. O'Farrell PH: High resolution two-dimensional electrophoresis of proteins. J Biol Chem 250: 4007-4021 (1975).
33. Palomares R, Herrmann RG, Oelmuller R: Antisense RNA for components associated with the oxygen-evolving complex and the Rieske iron/sulfur protein of the tobacco thyakoid membrane suppresses accumulation of mRNA, but not of protein. Planta 190: 305-312 (1993).
34. Perdew GH, Schaup HW, Selivonchic DP: The use of a zwitteronic detergent in two-dimensional gel electrophoresis of trout liver microsmes. Anal Biochem 135: 453-455 (1983).
35. Peterman TM, Goodman HM: The glutamine synthetase gene family of Arabidopsis thaliana: light-regulation and differential expression in leaves, roots and seeds. Mol Gen Genet 330: 145-154 (1991).
36. Re AB, Jones D, Learned RM: Co-expression of native and introduced genes reveals cryptic regulation of HMG CoA reductase expression in Arabidopsis. Plant J 7: 771-784 (1995).
37. Roche D, Temple SJ, Sengupta-Gopalan C: Two classes of differentially regulated glutamine synthetase genes are expressed in the soybean nodule: a nodule-specific class and a constitutively expressed class. Plant Mol Biol 22: 971-983 (1993).
38. Rodermel SR, bogorad L: Antisense mRNA inhibition of ribulose bisphosphate carboxylase: the most abundant protein in photosynthetic cells. In: Murray JAH (ed) Modern Cell Biology, vol. 11, Antisense RNA and DNA, pp. 121-135. Wiley-Liss, New York (1992).
39. Rogers SG, Klee HJ, Horsch RB, Fraley RT: Improved vectors for plant transformation: expression cassette vectors and new selectable markers. Meth Enzymol 153: 253-277 (1987).
40. Sambrook J, Fritsch EF, Maniatis T: Molecular Cloning: A Laboratory Manual, 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY (1989).
41. Sanangelatoni AM, Barbarini D, Pasquale GD, Cammarano P, Tiboni O: Cloning and nucleotide sequence of an archaebacterial glutamine synthetase gene: Phylogenetic implications. Mol Gen Genet 221: 187-194 (1990).
42. Shahin EA: Totipotency of tomato protoplasts. Theor Appl Genet 69: 235-240 (1985).
43. Sukanya R, Li M-G, Snustad DP: Root- and shoot-specific responses of individual glutamine synthetase genes of maize to nitrate and ammonium. Plant Mol Biol 26: 1935-1946 (1994).
44. Temple SJ, Bagga S, Sengupta-Gopalan C: Can glutamine synthetase activity levels be modulated in transgenic plants by the use of recombinant DNA technology? Biochem Soc Trans 22: 915-920 (1994).
45. Temple SJ, Heard J, Ganter G, Dunn K, Sengupta-Gopalan C: Characterization of a nodule-enhanced glutamine synthetase from alfalfa: nucleotide sequence, in situ localization and transcript analysis. Mol Plant-Microbe Interact 8: 218-227 (1995).
46. Temple SJ, Knight TJ, Unkefer PJ, Sengupta-Gopalan C: Modulation of glutamine synthetase gene expression in tobacco by the introduction of an alfalfa glutamine synthetase gene in sense and antisense orientation: molecular and biochemical analysis. Mol Gen Genet 236: 315-325 (1993).
47. Temple SJ, Kunjibettu S, Roche D, Sengupta-Gopalan C: Total glutamine synthetase activity during soybean nodule development is controlled at the level of transcription and holoprotein turnover. Plant Physiol 112: 1723-1733 (1996).
48. Walker EL, Coruzzi GM: Developmentally regulated expression of the gene family for cytosolic glutamine synthetase in Pisum sativum. Plant Physiol 91: 702-708 (1989).
49. Woods DR, Reid SJ: Recent developments on the regulation and structure of glutamine synthetase enzymes from selected bacterial groups. FEMS Microbiol Rev 11: 273-284 (1993).