Identification of peptides inhibiting enzyme I of the bacterial phosphotransferase system using combinatorial cellulose-bound peptide libraries

European Journal of Biochemistry

Volumn 254, Issue 2, 1998, Pages 433-438

  Mukhija, Seema ^a,b   Germeroth, Lothar ^c   Schneider Mergener, Jens ^d   Erni, Bernhard ^a  

^a UNIVERSITY OF BERN   (Switzerland)

^b Arpida Ltd   (Switzerland)

^c Jerini Bio Tools GmbH   (Germany)

^d CHARITÉ UNIVERSITÄTSMEDIZIN BERLIN   (Germany)

Author keywords

Active site inhibitor; Enzyme I; Peptide library; Phosphohistidine; Phosphotransferase system

Indexed keywords

BACTERIAL ENZYME; CARBOHYDRATE; CARRIER PROTEIN; HEPTAPEPTIDE; HISTIDINE; OCTAPEPTIDE; PEPTIDE LIBRARY; PHOSPHOENOLPYRUVATE SUGAR PHOSPHOTRANSFERASE; PHOSPHOPROTEIN;

ARTICLE; CONTROLLED STUDY; ENZYME INHIBITION; ENZYME SUBUNIT; ESCHERICHIA COLI; NONHUMAN; PHOSPHORYLATION AND DEPHOSPHORYLATION; PRIORITY JOURNAL; PROTEIN ANALYSIS; SIGNAL TRANSDUCTION;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; EUKARYOTA;

EID: 0032103218     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1432-1327.1998.2540433.x     Document Type: Article

References (28)

1. Desnottes, J. F. (1996) New targets and strategies for the development of antibacterial agents. Trends Biotechnol. 14, 134-140.
2. Saier, M. H. Jr & Reizer, J. (1994) The bacterial phosphotransferase system: New frontiers 30 years later, Mol. Microbiol. 13, 755-764.
3. Postma, P. W., Lengeler, J. W. & Jacobson, G. R. (1993) Phosphoenolpyruvate carbohydrate phosphotransferase systems of bacteria, Microbiol. Rev. 57, 543-594.
4. Nishikawa, Y., Scotland, S. M., Smith, H. R., Willshaw, G. A. & Rowe, B. (1995) Catabolite repression of the adhesion of Vero cytotoxin-producing Escherichia coli of serogroups 0157 and 0111, Microb. Pathog. 18, 223-229.
5. Curtiss, R. & Kelly, S. M. (1987) Salmonella typhimurium deletion mutants lacking adenylate cyclase and cyclic AMP receptor protein are avirulent and immunogenic, Infect. Immun. 55, 3035-3043.
6. Saier, M. H. Jr & Chin, M. (1990) Energetics of the bacterial phosphotransferase system in sugar transport and the regulation of carbon metabolism, in Bacterial energetics (Krulwich, T. A., ed.) pp. 273-299, Academic Press, London, New York.
7. Parr, T. R. & Saier, M. H. Jr (1992) The bacterial phosphotransferase system as a potential vehicle for the entry of novel antibiotics, Res. Microbiol. 143, 443-447.
8. Ammer, J., Brennenstuhl, M., Schindler, P., Holtje, J. V. & Zähner, H. (1979) Phosphorylation of streptozotocin during uptake via the phosphoenolpyruvate:sugar phosphotransferase system in Escherichia coli, Antimicmh. Agents Chemother. 16, 801-807.
9. Houghten, R. A. (1994) Combinatorial libraries. Finding the needles in the haystack, Curr. Biol. 4, 564-567.
10. Kramer, A. & Schneider-Mergener, J. (1998) Synthesis of peptide libraries on continuous cellulose membranes, Methods Mol. Biol. 87, 25-39.
11. Tegge, W., Frank, R., Hofmann, F. & Dostamnn, W. (1995) Determination of cyclic nucleotide-dependent protein kinase substrate specificity by use of peptide libraries on cellulose paper, Biochemistry 34, 10569-10577.
12. Lengeler, J., Auburger, A. M., Mayer, R. & Pecher, A. (1981) The phosphoenolpyruvate-dependent carbohydrate: phosphotransferase system enzymes II as chemoreceptors in chemotaxis of Escherichia coli, Mol. Gen. Genet. 183, 163-170.
13. Mao, Q., Schunk, T., Flükiger, K. & Erni, B. (1995) Functional reconstitution of the purified mannose phosphotransferase system of Escherichia coli into phospholipid vesicles, J. Biol. Chem. 270, 5258-5265.
14. 12P]phosphoenolpyruvate via the pyruvate kinase exchange reaction at equilibrium, Biochim. Biophys. Acta 760, 185-187.
15. Mukhija, S. & Erni, B. (1997) Phage display selection of peptides against enzyme I of the phosphoenolpyruvate-sugar phosphotransferase system (PTS), Mol. Microbiol. 25, 1159-1166.
16. 2+ affinity chromatography, and functional reconstitution of the transporter for N-acetyl glucosamine of Escherichia coli, J. Biol. Chem 271, 14819-14824.
17. Frank, R. (1992) Spot synthesis: an easy technique for the positionally addressable parallel chemical synthesis on a membrane support. Tetrahedron 48, 217-232.
18. Glc subunit, Biochemistry 36, 7408-7417.
19. Parkinson, J. S. & Kofoid, E. C. (1992) Communication modules in bacterial signaling proteins, Annu. Rev. Genet. 26, 71-112
20. Hess, J. F., Oosawa, N. K. & Simon, M. I. (1988) Phosphorylation of three proteins in the signaling pathway of bacterial chemotaxis, Cell 53, 79-87.
21. Liao, D. I., Silverton, E., Seok, Y. J., Lee, B. R., Peterkofsky, A. & Davies, D. R. (1996) The first step in sugar transport: Crystal structure of the amino terminal domain of enzyme I of the E. coli P-pyruvate: Sugar phosphotransferase system and a model of the phosphotransfer complex with HPr, Structure 4, 861-872.
22. Johnson, L. N. & Barford, D. (1993) The effects of phosphorylation on the structure and function of proteins, Annu. Rev. Biophys. Biomol. Struct. 22, 199-232.
23. Napper, S., Anderson, J. W., Georges, F., Quail, J. W., Delbaere, L. T. J. & Waygood, E. B. (1996) Mutation of serine-46 to aspartate in the histidine containing protein of Escherichia coli mimics the inactivation by phosphorylation of serine-46 in HPrs from grampositive bacteria, Biochemistry 35, 11260-11267.
24. Payne, J. W. & Gilvarg, C. (1968)Size distribution on peptide utilization in E. coli, J. Biol. Chem. 243, 6291-6299.
25. Oren, Z. & Shai, Y. (1997) Selective lysis of bacteria but not mammalian cells by diastereomers of mellitin: structure function study, Biochemistry 36, 1826-1835.
26. Blattner, F. R., Plunkett, G. III, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B. & Shao, Y. (1997) The complete genome sequence of Escherichia coli K-12, Science 277, 1453-1462.
27. Fleischmann, R. D., Adams, M. D., White, O., Clayton, R. A., Kirkness, E. F., Kerlavage, A. R., Bult, C. J., Tomb, J.-F., Dougherty, B. A., Merrick, J. M., McKenney, K., Sutton, G., FitzHugh, W., Fields, C. A., Gocayne, J. D., Scott, J. D., Shirley, R., Liu, L.-I., Glodek, A., Kelley, J. M., Weidman, J. F., Phillips, C. A., Spriggs, T., Hedblom, E., Cotton, M. D., Utterback, T. R., Hanna, M. C., Nguyen. D. T., Saudek, D. M., Brandon, R. C., Fine, L. D., Fritchman, J. L., Fuhrmann, J. L., Geoghagen, N. S. M., Gnehm, C. L., McDonald, L. A., Small, K. V., Fraser, C. M., Smith, H. O. & Venter, J. C. (1995) Whole-genome random sequencing and assembly of Haemophilus influenzae Rd, Science 269, 496-512.
28. Fraser, C. M., Gocayne, J. D., White, O., Adams, M. D., Clayton, R. A., Fleischmann, R. D., Bult, C. J., Kerlavage, A. R., Sutton, G., Kelley, J. M., Fritchman, J. L., Weidman, J. F., Small, K. V., Sandusky, M., Fuhrmann, J. L., Nguyen, D. T., Utterback, T. R., Saudek, D. M., Phillips, C. A., Merrick, J. M., Tomb, J. F., Dougherty, B. A., Bott, K. F., Hu, P. C., Lucier, T. S., Peterson S. N., Smith, H. O., Hutchison, C. A. & Venter, J. C. (1995) The minimal gene complement of Mycoplasma genitalium, Science 270, 397-403.