A heat-inducible Bacillus subtilis bacteriophage Φ105 expression system for the production of the protective antigen of Bacillus anthracis

FEMS Microbiology Letters

Volumn 163, Issue 1, 1998, Pages 43-47

  Baillie, L W J ^a   Moore, P ^a   McBride, B W ^b  

^a DEFENCE SCIENCE AND TECHNOLOGY LABORATORY   (United Kingdom)

^b PUBLIC HEALTH ENGLAND   (United Kingdom)

Author keywords

Bacillus subtilis; Expression; Prophage; Vaccine

Indexed keywords

ANTIGEN EXPRESSION; ARTICLE; BACILLUS ANTHRACIS; BACILLUS SUBTILIS; BACTERIOPHAGE; HEAT SENSITIVITY; HOST RESISTANCE; MOLECULAR STABILITY; NONHUMAN; PRIORITY JOURNAL;

ANIMALS; ANTHRAX; ANTIGENS, BACTERIAL; BACILLUS ANTHRACIS; BACILLUS PHAGES; BACILLUS SUBTILIS; BACTERIAL TOXINS; BACTERIAL VACCINES; FEMALE; GENE DOSAGE; GENE EXPRESSION REGULATION, VIRAL; GENES, BACTERIAL; GUINEA PIGS; HEAT; HUMANS; RECOMBINANT PROTEINS; SPORES, BACTERIAL; VACCINATION;

EID: 0032102861     PISSN: 03781097     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0378-1097(98)00149-9     Document Type: Article

References (16)

1. Thorne, C.B. (1993) Bacillus anthracis. In: Bacillus subtilis and Other Gram-positive Bacteria (Sonenshein, A.L., Hoch, J.A. and Losick, R., Eds.), pp. 113-124. American Society for Microbiology, Washington, DC.
2. Belton, F.C. and Strange, R.E. (1954) Studies on a protective antigen produced in vitro from Bacillus anthracis: medium and methods of production. Br. J. Exp. Pathol. 35, 144-152.
3. Leppla, S.H. (1991) The anthrax toxin complex. In: SourceBook of Bacterial Toxins (Alouf, J.E. and Freer, J.H., Eds.), pp. 277-302. Academic Press, New York.
4. Vodkin, M.H. and Leppla, S.H. (1983) Cloning of the protective antigen of Bacillus anthracis. Cell 34, 693-697.
5. Ivins, B.E. and Welkos, S.L. (1986) Cloning and expression of the Bacillus anthracis protective antigen gene in Bacillus subtilis. Infect. Immun. 54, 537-542.
6. Baillie, L.W.J., Moore, P. and Manchee, R.J. (1996) Development of a Bacillus subtilis based system for the expression of the protective antigen of Bacillus anthracis. In: Proceedings of the International Workshop on Anthrax, 19-21 September 1995, Winchester, UK. Salisbury Med. Bull. 87 (Special Suppl.), 133-135.
7. Baillie, L.W.J., Moore, P. and Manchee, R.J. (1996) Development of a Bacillus subtilis based system for the expression of the protective antigen of Bacillus anthracis. In: Proceedings of the International Workshop on Anthrax, 19-21 September 1995, Winchester, UK. Salisbury Med. Bull. 87 (Special Suppl.), 133-135.
8. Errington, J. (1990) Gene cloning techniques. In: Molecular Biological Methods for Bacillus (Harwood, C.R. and Cutting, S.M., Eds.), pp. 175-220. Wiley, Chichester.
9. Thornewell, S.J., East, A.K. and Errington, J. (1993) An efficient expression and secretion system based on Bacillus subtilis phage Φ105 and its use for the production of B.cereus β-lactamase I. Gene 133, 47-53.
10. Maniatis, T., Fritsch, E.F. and Sambrook, J. (1982) Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY.
11. Anaganostopoulos, C. and Spizizens, J. (1961) Requirements for transformation in Bacillus subtilis. J. Bacteriol. 81, 741-746.
12. Cutting, S.M. (1990) Genetic analysis. In: Molecular Biological Method for Bacillus (Harwood, C.R. and Cutting, S.M., Eds.), pp. 26-74. Wiley, Chichester.
13. McBride, B.W., Moog, A., Telfer, J.L., Lever, M.S., Miller, J., Turnbull, P.C.B. and Baillie, L. (1998) Protective efficacy of a recombinant antigen against Bacillus anthracis challenge and assessment of immunological markers. Vaccine (in press).
14. Witzmann, C. and Bohm, R. (1989) Specificity of monoclonal antibodies against vegetative cells of Bacillus anthracis. In: Proceedings of the International Workshop on Anthrax, 11-13 April 1989, Winchester, UK. Salisbury Med. Bull. 68 (Special Suppl.), 25-26.
15. Witzmann, C. and Bohm, R. (1989) Specificity of monoclonal antibodies against vegetative cells of Bacillus anthracis. In: Proceedings of the International Workshop on Anthrax, 11-13 April 1989, Winchester, UK. Salisbury Med. Bull. 68 (Special Suppl.), 25-26.
16. Bron, S. (1990) Plasmids. In: Molecular Biological Method for Bacillus (Harwood, C.R. and Cutting, S.M., Eds.), pp. 75-173. Wiley, Chichester.