5'-nucleotidase activity in lymphocytes from patients affected by B- cell chronic lymphocytic leukemia

Clinical Biochemistry

Volumn 31, Issue 4, 1998, Pages 269-272

  Rosi, Francesca ^a   Tabucchi, Antonella ^a   Carlucci, Filippo ^a   Galieni, Piero ^a   Lauria, Francesco ^a   Zanoni, Lorenzo ^a   Guerranti, Roberto ^a   Marinello, Enrico ^a   Pagani, Roberto ^a  

^a UNIVERSITY OF SIENA   (Italy)

Author keywords

5' nucleotidase; CD73; Enzymes; HPLC B CLL; Human lymphocytes; Immunofluorescence; Leukemia; Nucleotides; Peripheral blood lymphocytes

Indexed keywords

5' NUCLEOTIDASE;

ADULT; AGED; ARTICLE; B CELL LEUKEMIA; CHRONIC LYMPHATIC LEUKEMIA; CLINICAL ARTICLE; CONTROLLED STUDY; ENZYME ACTIVITY; HUMAN; HUMAN CELL; PERIPHERAL LYMPHOCYTE; PRIORITY JOURNAL; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; TRANSCRIPTION REGULATION;

5'-NUCLEOTIDASE; AGED; BLOTTING, NORTHERN; ENZYME ACTIVATION; FLUORESCENT ANTIBODY TECHNIQUE; HUMANS; ISOENZYMES; LEUKEMIA, B-CELL, CHRONIC; LYMPHOCYTES; MIDDLE AGED; POLYMERASE CHAIN REACTION; RNA, MESSENGER;

EID: 0032101723     PISSN: 00099120     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0009-9120(98)00017-4     Document Type: Article

References (18)

1. 1. Thompson LF, Ruedi JM, Glass A, et al. Production and characterization of monoclonal antibodies to the glycosylphosphatidylinositol-anchored lymphocyte differentiation antigen ecto-5'-nucleotidase (CD73). Tissue Antigens 1990; 35:9-19.
2. 2. Thompson LF, Resta R. CD73 and lymphocyte development. Medical Immunol 1994; 27:247-58.
3. 3. Zimmermann H. 5'-nucleotidase: molecular structure and functional aspects. Biochem J 1992; 285:345-65.
4. 4. Thompson LF, Ruedi JM, O'Connor RD, Bastian JF. Ecto-5'-nucleotidase expression during human B cell development. An explanation for the heterogeneity in B lymphocyte ecto-5'-nucleotidase activity in patients with hypogammaglobulinemia. J Immunol 1986;137: 2496-500.
5. 5. Edwards NL, Gelfand EW, Burk L, Dosch DM, Fox JH. Distribution of 5'-nucleotidase in human lymphoid tissues. Proc Natl Acad Sci 1979; 76:3473-6.
6. 6. Thompson LF, Ruedi JM, Glass A, Low MJ, Lucas AH. Antibodies to 5'-nucleotidase (CD73) a glycosyl-phosphatidylinositol-anchored protein, cause human peripheral blood T cells to proliferate. J Immunol 1989; 143:1815-21.
7. 7. Gutensohn W, Resta R, Misumi Y, Ikehara Y, Thompson LF. Ecto-5'-nucleotidase activity is not required for T cell activation through CD73. Cell Immunol 1995; 161:213-7.
8. 8. Hansen KR, Resta R, Webb CF, Thompson LF. Isolation and characterization of the promoter of the human 5'-nucleotidase (CD73)-encoding gene. Gene 1995; 167:307-12.
9. 9. Boyle JM, Hey Y, Grzeschik KH, Thompson LF, Munro E, Fox M. Regional localization of human ecto-5'-nucleotidase to chromosome 6 q14-q21. Hum Genet 1989; 83:179-80.
10. 10. Misumi Y, Ogata S, Ohkubo K, Ikehara Y. Primary structure of human placental 5'-nucleotidase and identification of the glycolipid anchor in the mature form. Eur J Biochem 1990; 191:563-9.
11. m" soluble 5'-nucleotidase in human tissues and rat liver. Adv Exp Med Biol 1988; 253B:129-34.
12. 12. Oka J, Matsumoto A, Hosokawa Y, Inoue S. Molecular cloning of human cytosolic purine 5'-nucleotidase. Biochem Biophys Res Comm 1994; 205(1):917-22.
13. 13. Böyum A. Isolation of leukocytes from blood and bone marrow. Scand J Clin Lab Invest 1968; 97:31-50.
14. 14. Brinchman JE, Vartdal F, Gaudernack G, et al. Direct immunomagnetic quantification of lymphocyte subsets in blood. Clin Exp Immunol 1988; 71:182-6.
15. 15. Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principles of protein-dye-binding. Anal Biochem 1976; 72:248-54.
16. 16. Harmsen E, De Jong JW, Serruys PW. Hypoxanthine production by ischemic heart demonstrated by high pressure liquid chromatography of blood purine nucleosides and oxypurines. Clin Chim Acta 1981; 115:73-84.
17. 17. Kummer U, Mysliwietz J, Gutenshon W, et al. Development and properties of a monoclonal antibody specific for human ecto-5'-nucleotidase. Immunobiol 1984; 166:203-11.
18. 18. Chomczinski P, Sacchi N. Preparation of total RNA from mammalian cells. Anal Biochem 1987; 162: 156-9.