Antibodies to protease-activated receptor 3 inhibit activation of mouse platelets by thrombin

Blood

Volumn 91, Issue 11, 1998, Pages 4152-4157

  Ishihara, Hiroaki ^a   Zeng, Dewan ^a   Connolly, Andrew J ^a   Tam, Carmen ^a   Coughlin, Shaun R ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

PROTEINASE; THROMBIN;

AMINO TERMINAL SEQUENCE; ANIMAL CELL; ARTICLE; CALCIUM CELL LEVEL; CALCIUM MOBILIZATION; ENZYME ACTIVATION; HEMOSTASIS; HYDROLYSIS; LIGAND BINDING; MEGAKARYOCYTE; MOUSE; NONHUMAN; PEPTIDE SYNTHESIS; PRIORITY JOURNAL; PROTEIN DOMAIN; PROTEIN EXPRESSION; THROMBOCYTE ACTIVATION; THROMBOCYTE METABOLISM;

EID: 0032101592     PISSN: 00064971     EISSN: None     Source Type: Journal    
DOI: 10.1182/blood.v91.11.4152     Document Type: Article

References (17)

1. Vu T-KH, Hung DT, Wheaton VI, Coughlin SR: Molecular cloning of a functional thrombin receptor reveals a novel proteolytic mechanism of receptor activation. Cell 64:1057, 1991
2. Huang R, Sorisky A, Church WR, Simons E, Rittenhouse SE: Thrombin receptor-directed ligand accounts for activation by thrombin of platelet phospholipase C and accumulation of 3-phosphorylated phosphoinositides. J Biol Chem 266:18435, 1991
3. Scarborough RM, Naughton M, Teng W, Hung DT, Rose J, Vu T-KH, Wheaton VI, Turck CW, Coughlin SR: Tethered ligand agonist peptides: Structural requirements for thrombin receptor activation reveal mechanism of proteolytic unmasking of agonist function. J Biol Chem 267:13146, 1992
4. Vassallo RRJ, Kieber-Emmons T, Cichowski K, Brass LF: Structure-function relationships in the activation of platelet thrombin receptors by receptor-derived peptides. J Biol Chem 267:6081, 1992
5. Vu T-KH, Wheaton VI, Hung DT, Coughlin SR: Domains specifying thrombin-receptor interaction. Nature 353:674, 1991
6. Liu L, Vu T-KH, Esmon CT, Coughlin SR: The region of the thrombin receptor resembling hirudin binds to thrombin and alters enzyme specificity. J Biol Chem 266:16977, 1991
7. Hung DT, Vu T-KH, Wheaton VI, Charo IF, Nelken NA, Esmon CT, Coughlin SR: "Mirror image" antagonists of thrombin-induced platelet activation based on thrombin receptor structure. J Clin Invest 89:444, 1992
8. Mathews II, Padmanabhan KP, Ganesh V, Tulinsky A, Ishii M, Chen J, Turck CW, Coughlin SR, Fenton JN: Crystallographic structures of thrombin complexed with thrombin receptor peptides: Existence of expected and novel binding modes. Biochemistry 33:3266, 1994
9. Chen J, Ishii M, Wang L, Ishii K, Coughlin SR: Thrombin receptor activation: Confirmation of the intramolecular tethered liganding hypothesis and discovery of an alternative intermolecular liganding mode. J Biol Chem 269:16041, 1994
10. Hung DT, Vu T-KH, Wheaton VI, Ishii K, Coughlin SR: The cloned platelet thrombin receptor is necessary for thrombin-induced platelet activation. Blocking antiserum to the thrombin receptor's hirudin-like domain. J Clin Invest 89:1350, 1992
11. Brass LF, Vassallo RJ, Belmonte E, Ahuja M, Cichowski K, Hoxie JA: Structure and function of the human platelet thrombin receptor. Studies using monoclonal antibodies directed against a defined domain within the receptor N terminus. J Biol Chem 267:13795, 1992
12. Connolly TM, Condra C, Feng DM, Cook JJ, Stranieri MT, Reilly CF, Nutt RF, Gould RJ: Species variability in platelet and other cellular responsiveness to thrombin receptor-derived peptides. Thromb Haemost 72:627, 1994
13. Derian CK, Santulli RJ, Tomko KA, Haertlein BJ, Andrade-Gordon P: Species differences in platelet responses to thrombin and SFLLRN. Receptor-mediated calcium mobilization and aggregation and regulation by protein kinases. Thromb Res 6:505, 1995
14. Connolly AJ, Ishihara H, Kahn ML, Farese RV, Coughlin SR: Role of the thrombin receptor in development and evidence for a second receptor. Nature 381:516, 1996
15. Ishihara H, Connolly AJ, Zeng D, Kahn ML, Zheng YW, Timmons C, Tram T, Coughlin SR: Protease-activated receptor 3 is a second thrombin receptor in humans. Nature 386:502, 1997
16. Harlow E, Lane D: Antibodies: A Laboratory Manual. Cold Spring Harbor, NY, Cold Spring Harbor Laboratory, 1988
17. Ishii K, Hein L, Kobilka B, Coughlin SR: Kinetics of thrombin receptor cleavage on intact cells: Relation to signaling. J Biol Chem 268:9780, 1993