The effect of dexamethasone on integrin and laminin expression in cultured human trabecular meshwork cells

Experimental Eye Research

Volumn 66, Issue 6, 1998, Pages 731-738

  Dickerson Jr , Jaime E ^a   Steely Jr , Thomas H ^a   English Wright, Sherry L ^a   Clark, Abbot F ^a  

^a ALCON RESEARCH LTD   (United States)

Author keywords

Dexamethasone; Glaucoma; Integrins; Laminin; Steroid induced glaucoma; Trabecular meshwork

Indexed keywords

DEXAMETHASONE; INTEGRIN; LAMININ; PROTEIN SUBUNIT;

ARTICLE; DRUG EFFECT; HUMAN; HUMAN CELL; IMMUNOBLOTTING; IMMUNOREACTIVITY; INTRAOCULAR PRESSURE; OPEN ANGLE GLAUCOMA; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN EXPRESSION; RADIOIMMUNOASSAY; RECEPTOR DOWN REGULATION; STEROID THERAPY; TRABECULAR MESHWORK;

EID: 0032096454     PISSN: 00144835     EISSN: None     Source Type: Journal    
DOI: 10.1006/exer.1997.0470     Document Type: Article

References (39)

1. Adams J. C., Watt F. M. Changes in keratinocyte adhesion during terminal differentiation: reduction in fibronectin binding precedes α5b1 integrin loss from the cell surface. Cell. 63:1990;425-435.
2. Alvarado J., Murphy C., Polansky J., Juster R. Age-related changes in trabecular meshwork cellularity. Invest. Ophthalmol. Vis. Sci. 21:1981;714-727.
3. Alvarado J., Murphy C., Juster R. Trabecular meshwork cellularity in primary open-angle glaucoma and non-glaucomatous normals. Ophthalmology. 91:1984;564-579.
4. Anderson D. R. Glaucoma: The damage caused by pressure: XLVI Edward Jackson Memorial Lecture. Am. J. Ophthalmol. 108:1989;485-495.
5. Babizhayev M. A., Brodskaya M. V. Fibronectin detection in drainage outflow system of human eyes in ageing and progression of open-angle glaucoma. Mech. Ageing Dev. 47:1989;145-157.
6. Bill A. The drainage of aqueous humor. Invest. Ophthalmol. 14:1975;1-3.
7. Burridge K., Faith K., Kelly T., Nacelles G., Turner C. Focal adhesions: Transmembrane junctions between the extracellular matrix and the cytoskeleton. Ann. Rev. Cell Biol. 4:1988;487-525.
8. Clark A. F. Steroids, ocular hypertension, and glaucoma. J. Glaucoma. 4:1995;354-369.
9. Clark A. F., Wilson K., McCartney M. D., Miggans S. T., Kunkle M., Howe W. Glucocorticoid-induced formation of cross-linked actin networks in human trabecular meshwork cells. Invest. Ophthalmol. Vis. Sci. 35:1994;281-294.
10. Clark A. F., Steely H. T., Dickerson J. E. Jr, English-Wright S. L., Stropki K., McCartney M. D., Clark J. I., Matsushima H., Peskin E., Leverenz J., Fingert J. H., Stone E. M. Glucocorticoid induction of a glaucoma gene (GLC1A) in the human and monkey trabecular meshwork. Invest. Ophthalmol. Vis. Sci. (submitted). 1998.
11. Engel J. Laminins and other strange proteins. Biochem. 31:1992;10643-10651.
12. Floyd B. B., Cleveland P. H., Worthen D. M. Fibronectin in human trabecular drainage channels. Invest. Ophthalmol. Vis. Sci. 26:1985;797-804.
13. Francois J. The importance of mucopolysaccharides in intraocular pressure regulation. Invest. Ophthalmol. 14:1975;173-176.
14. Fujisawa N. Dexamethasone effects of protein synthesis on organ-cultured human trabecular meshwork: autoradiographical, biochemical, and immunohistochemical study. Nippon Ganka Gakkai Zasshi. 98:1994;31-37.
15. Grierson I., Howes R. Age related depletion of the cell population in the human trabecular meshwork. Eye. 1:1987;204-210.
16. Hemler M. E. Integrins. Guidebook to the Extracellular Matrix and Adhesion Proteins. 1993;Oxford University Press. p. 143-5.
17. Hynes R. O. Integrins: Versatility, modulation and signaling in cell adhesion. Cell. 69:1992;11-25.
18. Johnson D., Gottanka J., Flügel C., Hoffman F., Futa R., Lütjen-Drecoll E. Ultrastructural changes in the trabecular meshwork of human eyes treated with corticosteroids. Arch. Ophthalmol. 115:1997;375-383.
19. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 227:1970;680-685.
20. Maldonado B. A., Furcht L. T. Epidermal growth factor receptor stimulates integrin-mediated cell migration of cultured human corneal epithelial cells on fibronectin and arginine-glycine-aspartic acid peptide. Invest. Ophthalmol. Vis. Sci. 36:1995;2120-2126.
21. Matsumoto Y., Johnson D. H. Dexamethasone decreases phagocytosis by human trabecular meshwork cells in situ. Invest. Ophthalmol. Vis. Sci. 38:1997;1902-1907.
22. Newham P., Humphries M. J. Integrin adhesion receptors: structure, function and implications for biomedicine. Mol. Med. Today. 2:1996;304-313.
23. Nicholson L. J., Watt F. M. Decreased expression of fibronectin and the α5b1 integrin during terminal differentiation of human keratinocytes. J. Cell Sci. 98:1991;225-232.
24. O'Brien E. T., Perkins S. L., Roberts B. C., Epstein D. L. Dexamethasone inhibits trabecular cell retraction. Exp. Eye Res. 62:1996;675-688.
25. Polansky J. R, Kurtz R. M, Fauss D. J, Kim R. Y., Bloom E. In vitro correlates of glucocorticoid effects on intraocular pressure. Krieglstein G. K. Glaucoma Update IV. 1991;20-29 Springer-Verlag, Berlin.
26. Putney L. K., Brandt J. D., O'Donnell M. E. Effects of dexamethasone on sodium-potassium-chloride cotransport in trabecular meshwork cells. Invest. Ophthalmol. Vis. Sci. 38:1997;1229-1240.
27. Rodrigues M. M., Katz S. I., Foidart J.-M., Spaeth G. L. Collagen, factor VIII antigen, and immunoglobulins in the human aqueous drainage channels. Ophthalmology. 87:1980;337-345.
28. Rohen J. W. Why is intraocular pressure elevated in chronic simple glaucoma? Anatomical Considerations. Ophthalmology. 90:1983;758-765.
29. Snyder R. W., Stamer W. D., Kramer T. R., Seftor R. E. B. Corticosteroid treatment and trabecular meshwork proteases in cell and organ culture supernatants. Exp. Eye Res. 57:1993;461-468.
30. Sommer A., Tielsch J. M., Katz J., Quigley H. A., Gottsch J. D., Javitt J. C., Martone J. F., Royall R. M., Witt K. A., Ezrine S. Racial differences in the cause-specific prevalence of blindness in east Baltimore. N. Engl. J. Med. 325:1991;1412-1417.
31. Spaeth G. L., Rodrigues M. M., Seinseb S. Steroid-induced glaucoma. Trans. Am. Ophthalmol. Soc. LXXV:1977;353-381.
32. Steely H. T. Jr, Browder S. L., Julian M. B., Miggans S. T., Wilson K. L., Clark A. F. The effects of dexamethasone on fibronectin expression in cultured human trabecular meshwork cells. Invest. Ophthalmol. Vis. Sci. 33:1992;2242-2250.
33. Stone E. M., Fingert J. H., Alward W. L. M., Nguyen T. D., Polansky J. R., Sunden S. L. F., Nishimura D., Clark A. F., Nystuen A., Nichols B. E., Mackey D. A., Ritch R., Kalenak J. W., Craven E. R., Sheffield V. C. Identification of a gene that causes primary open angle glaucoma. Science. 275:1997;668-670.
34. Tervo K., Paallysaho T., Virtanen I., Tervo T. Integrins in human anterior chamber angle. Graefe's Arch. Clin. Exp. Ophthalmol. 233:1995;291-295.
35. Timpl R., Rohide H., Risteli L., Ott U., Robey P. G., Martin G. R. Laminin. Meth. Enzymol. 82:1982;831-838.
36. Towbin H., Staehilin T., Gordon G. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: Procedure and some applications. Proc. Natl. Acad. Sci. U.S.A. 76:1979;4350-4354.
37. Wilson K., McCartney M. D., Miggans S. T., Clark A. F. Dexamethasone induced ultrastructural changes in cultured human trabecular meshwork cells. Curr. Eye Res. 12:1993;783-793.
38. Yue, B. and Zhou, L. (1995). Effects of dexamethasone on the levels of fibronectin, laminin and collagen types I and IV in the bovine trabecular meshwork. (Abstract) Invest. Ophthalmol. Vis. Sci. 36 (suppl.), 3349.
39. Yun A. J., Murphy C. G., Polansky J. R., Newsome D. A., Alvarado J. A. Proteins secreted by trabecular meshwork cells: glucocorticoid and other effects. Invest. Ophthalmol. Vis. Sci. 30:1989;2012-2022.