Enzymatic mutation detection. Phosphate ions increase incision efficiency of endonuclease VII at a variety of damage sites in DNA

Mutation Research - Mutation Research Genomics

Volumn 382, Issue 3-4, 1998, Pages 85-92

  Golz, Stefan ^a   Greger, Beate ^a   Kemper, Börries ^a  

^a UNIVERSITY OF COLOGNE   (Germany)

Author keywords

DNA secondary structure; Endonuclease VII; Enzymatic mutation detection; Enzyme reaction; Heteroduplex DNA; Mismatching bases; Phage T4; Phosphate ion; Reaction condition; Synthetic oligonucleotides

Indexed keywords

ENDONUCLEASE;

ARTICLE; BASE MISPAIRING; DNA DAMAGE; DNA DETERMINATION; DNA STRUCTURE; GENE MUTATION; PRIORITY JOURNAL;

EID: 0032078603     PISSN: 13835726     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1383-5726(97)00011-3     Document Type: Article

References (16)

1. [1] B. Kemper, M. Garabett, U. Courage, Studies on T4-head maturation. 2. Substrate specificity of gene-49-controlled endonuclease, Eur. J. Biochem. 115 (1981) 133-141.
2. [2] P.C. Solaro, K. Birkenkamp, P. Pfeiffer et al., Endonuclease VII of phage T4 triggers mismatch correction in vitro, J. Mol. Biol. 230 (1993) 868-877.
3. [3] G. Mosig, M. Shaw, G.M. Garcia, On the role of DNA replication, endonuclease VII, and rII proteins in processing of recombinational intermediates in phage T4, Cold Spring Harbor Symp. Quant. Biol. 49 (1984) 371-382.
4. [4] S.M. Grebenshchikova, L.A. Plugina, V.P. Scherbakov, The role of T4-bacteriophage endonuclease-VII in correction of mismatched regions, Genetika 30 (1994) 622-626.
5. [5] B. Kemper, 1997, Cruciform resolving enzymes, in DNA damage and repair, in: J.A. Nickoloff, M. Hoekstra (eds.), Biochemistry, Genetics and Cell Biology, Humana Press, Totowa.
6. [6] R. Youil, B.W. Kemper, R.G.H. Cotton, Screening for mutations by enzyme mismatch cleavage using T4 endonuclease VII, Proc. Natl. Acad. Sci. USA 92 (1995) 87-91.
7. [7] C. Giuntal, R. Youil et al., P53 and BRCAI mutation detection using the enzyme mismatch cleavage (EMC) method, Am. J. Hum. Genet. 57 (4) (1995) 342, abstract.
8. [8] R. Youil, B. Kemper, R.G.H. Cotton, Detection of 81 of 81 known mouse b-globin promoter mutations with T4 endonuclease VII - The EMC method, Genomics 32 (1996) 431-435.
9. [9] S. Pottmeyer, B. Kemper, T4 endonuclease VII resolves cruciform DNA with nick and counter-nick and its activity is directed by local nucleotide sequence, J. Mol. Biol. 223 (1992) 607-615.
10. [10] B. Kemper, M. Garabett, Studies on T4-head maturation. 1. Purification and characterization of gene-49-controlled endonuclease, Eur. J. Biochem. 115 (1981) 123-131.
11. [11] S. Golz, R. Birkenbihl, B. Kemper, Improved large scale preparation of phage T4 endonuclease VII overexpressed in E. coli, DNA Res. 2 (1995) 277-284.
12. [12] D.M.J. Lilley, B. Kemper, Cruciform-resolvase interactions in supercoiled DNA, Cell 36 (1984) 413-422.
13. [13] A. Bhattacharyya, A.I.H. Murchie, E. v. Kitzing et al., Model for the interaction of DNA junctions and resolving enzymes, J. Mol. Biol. 221 (1991) 1191-1207.
14. [14] J.A. Pikkemaat, F.J.J. Overmars, C.M. Dreef-Tromp et al., Conformational aspects of an unbranched phosphate analogon built in at the branch-point of a DNA four-way junction, J. Mol. Biol. 262 (1996) 357.
15. [15] H.M. Wu, D.M. Crothers, The locus of sequence-directed and protein-induced DNA bending, Nature 308 (1984) 509-513.
16. [16] H.S. Koo, D.M. Crothers, Calibration of DNA curvature and a unified description of sequence-directed bending, Proc. Natl. Acad. Sci USA. 85 (1988) 1763-1767.