Enhanced green fluorescent protein as an efficient reporter gene for retroviral transduction of human multipotent lymphoid precursors

Human Gene Therapy

Volumn 9, Issue 7, 1998, Pages 1103-1109

  Ramiro, Almudena R ^a   De Yébenes, Virginia G ^a   Trigueros, César ^a   Carrasco, Yolanda R ^a   Toribio, Maria L ^a  

^a UNIVERSIDAD AUTÓNOMA DE MADRID   (Spain)

Author keywords

[No Author keywords available]

Indexed keywords

CD34 ANTIGEN; GREEN FLUORESCENT PROTEIN; MUTANT PROTEIN;

ARTICLE; CELL STRAIN 3T3; CONTROLLED STUDY; DENDRITIC CELL; FLOW CYTOMETRY; GENE EXPRESSION; GENETIC TRANSDUCTION; HUMAN; HUMAN CELL; LEUKEMIA CELL LINE; LYMPHOID CELL; MARKER GENE; MICROSCOPY; REPORTER GENE; RETROVIRUS; THYMOCYTE;

UNIDENTIFIED RETROVIRUS;

EID: 0032078128     PISSN: 10430342     EISSN: None     Source Type: Journal    
DOI: 10.1089/hum.1998.9.7-1103     Document Type: Article

References (13)

1. BAGNIS, C., CHISCHPORTICH, C., IMBER, A.-M., VAN DEN BROECKE, A., CORNET, V., and MANNONI, P. (1997). Efficiency of retroviral transduction into hematopoietic cells by cocultivation procedure does not correlate with viral titer. Can. Gene Ther. 4, 5-8.
2. BIERHUIZEN, M.F.A., WESTERMAN, Y., VISSER, T.P., WOGNUM, A.W., and WAGEMAKER, G. (1997a). Green fluorescent protein variants as markers for retroviral-mediated gene transfer in primary hematopoietic cells and cell lines. Biochem. Biophys. Res. Commun. 234, 371-375.
3. BIERHUIZEN, M.F.A., WESTERMAN, Y., VISSER, T.P., DIMJATI, W., WOGNUM, A.W., and WAGEMAKER, G. (1977b). Enhanced green fluorescent protein as a selectable marker of retroviral-mediated gene transfer in immature hematopoietic bone marrow cells. Blood 90, 3304-3315.
4. BRENNER, K.M. (1996). Gene transfer to hematopoietic cells. N. Engl. J. Med. 335, 337-339.
5. BRENNER, M.K., RILL, D.R., HOLLADAY, M.S., HESLOP, H.E., MOEN, R.C., BUSCHLE, M., KRANCE, R.A., SANTANA, V.M., ANDERSON, W.F., and IHLE, J.N. (1993). Gene marking to determine whether autologous marrow infusion restores long-term haemopoiesis in cancer patients. Lancet 342, 1134-1137.
6. + cord blood cells is expressed by their T-cell progeny in the SCID-hu thymus. Blood 88, 107-113.
7. CONNEALLY, E., BARDY, P., EAVES, C.J., THOMAS, T., SHPALL, E.J., and HUMPHRIES, R.K. (1996). Rapid and efficient selection of human hematopoietic cells expressing murine heat-stable antigen as an indicator of retroviral-mediated gene transfer. Blood 87, 456-464.
8. CORMACK, B.P., VALDIVIA, R.H., and FALKOW, S. (1996). FACS-oplimized mutants of the green fluorescent protein (GFP). Gene 173, 33-38.
9. CUBITT, A.B., HEIM, R., ADAMS, S.R., BOYD, A.E., GROSS, L.A., and TSIEN, R.Y. (1995). Understanding, improving and using green fluorescent proteins. Trends Biochem. Sci. 20, 448-455.
10. HANAZONO, Y., YU, J.-M., DUNBAR, C.E., and EMMONS, R.V.B. (1997). Green fluorescent protein retroviral vectors: Low titer and high recombination frequency suggest a selective disadvantage. Hum. Gene Ther. 8, 1313-1319.
11. dim cells in the human thymus can differentiate into T, natural killer, and dendritic cells but are distinct from pluripotent stem cells. Blood 87, 5196-5206.
12. SHORTMAN, K., and WU, L. (1996). Early T lymphocyte progenitors. Annu. Rev. Inimunol. 14, 29-47.
13. VERMA, I.M., and SOMIA, N. (1997). Gene-therapy - promises, problems and prospects. Nature (London) 389, 239-242.