DNA single strand breaks and adenine nucleotide depletion as indices of oxidant effects on human lung cells

Free Radical Biology and Medicine

Volumn 24, Issue 7-8, 1998, Pages 1088-1096

  Ollikainen, Tiina R J ^a   Linnainmaa, Kaija I ^a   Raivio, Kari O ^b   Kinnula, Vuokko L ^c  

^a FINNISH INSTITUTE OF OCCUPATIONAL HEALTH   (Finland)

^b UNIVERSITY OF HELSINKI   (Finland)

^c UNIVERSITY OF OULU   (Finland)

Author keywords

ATP; Bronchial epithelial cells; COMET (SCGE); Free radical; H2O2; Menadione; Mesothelial cells; Nucleotides; Oxidants

Indexed keywords

ADENINE NUCLEOTIDE; ADENOSINE DIPHOSPHATE; ADENOSINE TRIPHOSPHATE; ANTIOXIDANT; FREE RADICAL; GLUTATHIONE; HYPOXANTHINE; LACTATE DEHYDROGENASE; MENADIONE; URIC ACID; XANTHINE;

ARTICLE; CELL CULTURE; CELL DAMAGE; DNA DAMAGE; DNA STRAND BREAKAGE; ENZYME ACTIVITY; GEL ELECTROPHORESIS; HUMAN; HUMAN CELL; LUNG ALVEOLUS CELL; MESOTHELIUM CELL; PRIORITY JOURNAL;

ADENINE NUCLEOTIDES; ANTIOXIDANTS; CELL LINE, TRANSFORMED; DNA DAMAGE; DNA, SINGLE-STRANDED; GLUTATHIONE; HUMANS; HYDROGEN PEROXIDE; L-LACTATE DEHYDROGENASE; LUNG; OXIDANTS; VITAMIN K;

EID: 0032078035     PISSN: 08915849     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0891-5849(97)00394-8     Document Type: Article

References (28)

1. Anderson D., Yu T.-W., Phillips B. J., Schmezer P. The effect of various antioxidants and other modifying agents on oxygen-radical-generated DNA damage in human lymphocytes in the COMET assay. Mutat. Res. 307:1994;261-271.
2. Churg A., Keeling B., Gilks B., Porter S., Olive P. Rat mesothelial and tracheal epithelial cells show equal DNA sensitivity to hydrogen peroxide-induced oxidant injury. Am. J. Physiol. 268:1995;832-838.
3. Fairbairn D. W., Olive P. L., O'Neill K. L. The comet assay A comprehensive review . Mutat. Res. 339:1995;37-39.
4. Olive P. L., Frazer G., Banàth J. P. Radiation-induced apoptosis measured in TK6 human B lymphoblast cells using the comet assay. Radiat. Res. 136:1993;130-136.
5. Müller W.-U., Bauch T., Streffer C., Niedereichholz F., Böcker W. Comet assay studies of radiation-induced DNA damage and repair in various tumor cell lines. Int. J. Radiat. Biol. 65:1994;315-319.
6. Collins A. R., Dobson V. L., Dušinská M., Kennedy G., Štêtina R. The comet assay What can it really tell us? Mutat. Res. 375:1997;183-193.
7. Hellman B., Vaghef H., Boström B. The concepts of tail moment and tail inertia in the single cell gel electrophoresis assay. Mutat. Res. 336:1995;123-131.
8. McKelvey-Martin V. J., Green M. H. L., Schmezer P., Pool-Zobel B. L., De Méo M. P., Collins A. The single cell gel electrophoresis assay (comet assay) A European review . Mutat. Res. 288:1993;47-63.
9. Ke Y., Reddel R. R., Gerwin B. I., Reddel H. K., Somers A. N. A., McMenamin M. G., LaVeck M. A., Stahel L. A., Lechner J. F., Harris C. C. Establishment of a human in vitro mesothelial cell model system for investigating mechanisms of asbestos-induced mesothelioma. Am. J. Pathol. 134:1989;979-991.
10. Linnainmaa K., Pelin K., Vanhala E., Tuomi T., Piccoli C., Fitzerald D. J., Yamasaki H. Gap junctional intercellular communication of primary and asbestos-associated malignant human mesothelial cells. Carcinogenesis. 14:1993;1597-1602.
11. Reddel R. R., Ke Y., Gerwin B. I., McMenamin M. G., Lechner J. F., Su R. T., Brash D. E., Park J. B., Rhim J. S., Harris C. C. Transformation of human bronchial epithelial cells by infection with SV 40 or adenovirus-12 SV40 hybrid virus, or transfection via strontium phosphate coprecipitation with a plasmid containing SV40 early region genes. Cancer Res. 48:1988;1904-1909.
12. Singh N. P., Tice R. R., Stephens R. E., Schneider E. L. A microgel electrophoresis techinque for the direct quantitation of DNA damage and repair in individual fibroblasts cultured on microscope slides. Mutat. Res. 252:1991;289-296.
13. Aalto K., Raivio K. O. Adenine nucleotide depletion from endothelial cells exposed to xanthine oxidase. Am. J. Physiol. 259:1990;C883-C888.
14. Bergmeyer H. U. Lactate dehydrogenase assay with pyruvate and NADH. Bergmeyer H. U. Methods of enzymatic analysis, Vol. 2. 1974;574-579 Academic Press, New York.
15. McCord J. M., Fridovich I. Superoxide dismutase An enzymatic function for erythrocuprein (hemocuprein) . J. Biol Chem. 244:1969;6049-6055.
16. Kinnula V. L., Everitt J. I., Mangum J. B., Chang L. Y., Crapo J. D. Antioxidant defense mechanisms in cultured pleural mesothelial cells. Am. J. Respir. Cell Mol. Biol. 7:1992;95-103.
17. Beutler E. Glutathione peroxidase. Beutler E. Red cell metabolism A manual of biochemical methods . 1975;71-73 Grune & Stratton, New York.
18. Frei B., Winterhalter K. H., Richter C. Menadione-(2-methyl-1,4-napthoquinone) dependent enzymatic redox cycling and calcium release by mitochondria. Biochemistry. 25:1986;4438-4443.
19. Spencer J. P. E., Jenner A., Aruoma O. I., Cross C. E., Wu R., Halliwell B. Oxidative DNA damage in human respiratory tract epithelial cells. Time course in relation to DNA strand breakage. Biochem. Biophys. Res. Commun. 224:1996;17-22.
20. Schraufstätter I. U., Hyslop P. A., Hinshaw D. B., Spragg R. G., Sklar L. A., Cochrane C. G. Hydrogen peroxide-induced injury of cells and its prevention by inhibitors of poly (ADP-ribose) polymerase. Proc. Natl. Acad. Sci. USA. 83:1986;4908-4912.
21. Kossenjans W., Rymaszewski Z., Barankiewicz J., Bobst A., Ashraf M. Menadione-induced oxidative stress in bovine heart mircrovascular endothelial cells. Microcirculation. 3:1996;39-47.
22. Andreoli S. P., Mallet C. P., McAteer J. A., Williams L. V. Antioxidant defense mechanism of endothelial cells and renal tubular epithelial cells in vitro - role of the glutathione redox cycle and catalase. Pediatr. Res. 32:1992;360-365.
23. Aalto K., Raivio K. O., Pietarinen P., Kinnula V. L. Intracellular high energy metabolite depletion and cell membrane injury with antioxidant enzymes during oxidant exposure in vitro. Toxicol. Lett. 85:1996;93-99.
24. Lee J.-G., Madden M. C., Reed W., Adler K., Devlin R. The use of the single cell gel electrophoresis assay in detecting DNA single strand breaks in lung cells in vitro. Toxicol. Appl. Pharmacol. 141:1996;195-204.
25. Lechner J. F., Tokimwa T., LaVeck M., Benedict W. F., Banks-Schlegel S., Yeager H., Banerjee A., Harris C. C. Asbestos-associated chromosomal changes in human mesothelial cells. Proc. Natl. Acad. Sci. USA. 82:1985;3884-3888.
26. Aalto K., Raivio K. O., Linnainmaa K., Virtanen T., Kinnula V. L. Sensitivity of human bronchial epithelial cells and pleural mesothelial cells to reactive oxygen species in vitro. (Abstr.). Am. Rev. Respir. Dis. 147:1993;A924.
27. Kinnula V. L., Chang L., Everitt J. I., Crapo J. D. Oxidants and antioxidants in alveolar epithelial type II cells In situ, freshly isolated and cultured cells . Am. J. Physiol. (Lung Cell Mol. Physiol.). 262:1992;L69-L77.
28. Kinnula V. L., Yankaskas J. R., Chang L., Virtanen I., Linnala A., Crapo J. D. Primary and immortalized (BEAS 2B) human bronchial epithelial cells have significant antioxidative capacity in vitro. Am. J. Respir. Cell Mol. Biol. 11:1994;568-576.