Polypeptide composition of envelopes of spinach chloroplasts: Two major proteins occupy 90% of outer envelope membranes

Plant and Cell Physiology

Volumn 39, Issue 5, 1998, Pages 526-532

  Koike, Hiroyuki ^b   Yoshio, Maki ^a,b   Kashino, Yasuhiro ^b   Satoh, Kazuhiko ^b  

^a NATIONAL INSTITUTE OF INFORMATION AND COMMUNICATIONS TECHNOLOGY   (Japan)

^b UNIVERSITY OF HYOGO   (Japan)

Author keywords

Chloroplast envelope; SDS PAGE; Spinach; Sucrose density gradient centrifugation

Indexed keywords

CENTRIFUGATION; CHLOROPLAST; MEMBRANE; PEPTIDE ANALYSIS; POLYACRYLAMIDE GEL ELECTROPHORESIS; RIBULOSEBISPHOSPHATE CARBOXYLASE; THYLAKOID;

PISUM SATIVUM; SPINACIA OLERACEA;

EID: 0032077317     PISSN: 00320781     EISSN: None     Source Type: Journal    
DOI: 10.1093/oxfordjournals.pcp.a029400     Document Type: Article

References (36)

1. Altschul, S.F., Gish, W., Miller, W., Meyers, E.W. and Lipman, D.J. (1990) Basic local alignment search tool. J. Mol. Biol. 215: 403-410.
2. Andrews, J. and Keegstra, K. (1983) Acyl-CoA synthetase is located in the outer membrane and acyl-CoA thioesterase in the inner membrane of pea chloroplast envelopes. Plant Physiol. 72: 735-740.
3. Andrews, J., Ohlrogge, J.B. and Keegstra, K. (1985) Final step of phosphatidic acid synthesis in pea chloroplasts occurs in the inner envelope membrane. Plant Physiol. 78: 459-465.
4. Block, M.A., Dorne, A.J., Joyard, J. and Douce, R. (1983) Preparation and characterization of membrane fractions enriched in outer and inner envelope membranes from spinach chloroplasts. II. Biochemical characterization. J. Biol. Chem. 258: 13281-13286.
5. Cline, K., Andrews, J., Mersey, B., Newcomb, E.H. and Keegstra, K. (1981) Separation and characterization of inner and outer envelope membranes of pea chloroplasts. Proc. Natl. Acad. Sci. USA 78: 3595-3599.
6. Cline, K. and Keegstra, K. (1983) Galactosyltransferases involved in galactolipid biosynthesis are located in the outer membrane of pea chloroplast envelopes. Plant Physiol. 71: 366-372.
7. Cline, K., Keegstra, K. and Staehelin, L.A. (1985) Freeze-fracture electron microscopic analysis of ultra rapidly frozen envelope membranes on intact chloroplasts and after purification. Protoplasma 125: 111-123.
8. Cline, K. and Henry, R. (1996) Import and routing of nucleus-encoded chloroplast proteins. Annu. Rev. Cell Dev. Biol. 12: 1-26.
9. Douce, R. and Joyard, J. (1982) Purification of the chloroplast envelope. In Methods in Chloroplast Molecular Biology. Edited by Edelman, M., Hallick, R.B. and Chua, N.H. pp. 239-256. Elsevier Biomedical Press, Amsterdam.
10. Douce, R. and Joyard, J. (1990) Biochemistry and function of the plastid envelope. Annu. Rev. Cell Biol. 6: 173-216.
11. Dress-Werringloer, U., Fischer, K., Wachter, E., Link, T.A. and Flügge, U. (1991) cDNA sequence and deduced amino acid sequence of the precursor of the 37 kDa inner envelope membrane polypeptide from spinach chloroplasts. Its transit peptide contains an amphiphalic alpha-helix as the only detectable structural element. Eur. J. Biochem. 195: 361-368.
12. Flügge, U.I. and Benz, R. (1984) Pore-forming activity in the outer membrane of the chloroplast envelope. FEBS Lett. 169: 85-89.
13. Flügge, U.I., Fischer, K., Gross, A., Sebald, W., Lottspeich, F. and Eckerskorn, C. (1989) The triose phosphate-3-phosphoglycerate-phosphate translocator from spinach chloroplast: nucleotide sequence of a full-length cDNA clone and import of the in vitro synthesized precursor protein into chloroplasts. EMBO J. 8: 39-46.
14. Flügge, U.I. and Heldt, H.W. (1991) Metabolite transportors of the chloroplast envelope. Annu. Rev. Plant Physiol. Plant Mol. Biol. 42: 129-144.
15. Hirsch, S., Muckel, E., Heemeyer, F., von Heijne, G. and Soll, J. (1994) A receptor component of the chloroplast protein translocation machinery. Science 266: 1989-1992.
16. Hirsch, S. and Soll, J. (1995) Import of a new chloroplast inner envelope protein is greatly stimulated by potassium phosphate. Plant Mol. Biol. 27: 1173-1181.
17. Ikeuchi, M. and Inoue, Y. (1988) A new 4.8-kDa polypeptide intrinsic to the PSII reaction center as revealed by modified SDS-PAGE with improved resolution of low-molecular-weight proteins. Plant Cell Physiol. 29: 1233-1239.
18. Joyard, J., Billecocq, A., Bartlett, S.G., Block, M.A., Chua, N.-H. and Douce, R. (1983) Localization of polypeptides to the cytosolic side of the outer envelope membrane of spinach chloroplasts. J. Biol. Chem. 258: 10000-10006.
19. Keegstra, K. and Yousif, A.E. (1986) Isolation and characterization of chloroplast envelope membranes. Methods Enzymol. 118: 316-325.
20. Kessler, F., Blobel, G., Patel, H.A. and Schnell, D.J. (1994) Identification of two GTP-binding proteins in the chloroplast protein import machinery. Science 266: 1035-1039.
21. Laemmli, U.K. (1970) Cleavage of structural protein during the assembly of the head of bacteriophage T4. Nature 227: 680-685.
22. Lipman, D.J. and Pearson, W.R. (1985) Rapid and sensitive protein similarity searches. Science 227: 1435-1441.
23. Mackender, R.O. and Leech, R.M. (1970) Isolation of chloroplast envelope membranes. Science 228: 1347-1348.
24. McCarty, D.R., Keegstra, K. and Selman, B.R. (1984) Characterization of the ATPase associated with pea chloroplast envelope membranes. Plant Physiol. 76: 584-588.
25. Pain, D., Kanwar, Y.S. and Blobel, G. (1988) Identification of a receptor for protein import into chloroplasts and its localization to envelope contact zones. Nature 331: 232-237.
26. Pohlmeyer, K., Soll, J., Steinkemp, T., Hinnah, S. and Wagner, R. (1997) Isolation and characterization of an amino acid-selective channel protein present in the chloroplastic outer envelope membrane. Proc. Natl. Acad. Sci. USA 94: 9504-9609.
27. Robinson, S.P. (1985) Osmotic adjustment by intact isolated chloroplasts in response to osmotic stress and its effect on photosynthesis and chloroplast volume. Plant Physiol. 79: 996-1001.
28. Salomon, M., Fischer, K., Flügge, U.I. and Soll, J. (1990) Sequence analysis and protein import studies of an outer chloroplast envelope polypeptide. Proc. Natl. Acad. Sci. USA 87: 5778-5782.
29. Schnell, D.J. and Blobel, G. (1993) Identification of intermediates in the pathway of protein import into chloroplasts and their localization to envelope contact sites. J. Cell Biol. 120: 103-115.
30. Schnell, D.J., Kessler, F. and Blobel, G. (1994) Isolation of components of the chloroplast import machinery. Science 266: 1007-1012.
31. Scott, S.V. and Theg, S.M. (1996) A new intermediate on the chloroplast protein import pathway reveals distinct translocation machineries in the two envelope membranes: energetics and mechanistic implications. J. Cell Biol. 132: 63-75.
32. Seedorf, M., Wagemann, K. and Soll, J. (1995) A constituent of the chloroplast import complex represents a new type of GTP-binding protein. Plant J. 1: 401-411.
33. Siegenthaler, P.-A. and Dumont, N. (1990) Characteristics of spinach chloroplast envelope; Thylakoid and stromal polypeptides as revealed by Triton X-114 phase partition. Plant Cell Physiol. 31: 1101-1108.
34. Tranel, P.J., Froehlich, J., Goyal, A. and Keegstra, K. (1995) A component of the chloroplastic protein import apparatus is targeted to the outer envelope membrane via a novel pathway. EMBO J. 14: 2436-2446.
35. Wu, C., Seibert, F.S. and Ko, K. (1994) Identification of chloroplast envelope proteins in close physical proximity to a partially translocated chimeric precursor protein. J. Biol. Chem. 269: 32264-32271.
36. Yoshio, M., Kashino, Y., Koike, H. and Satoh, K. (1995) Identification and localization of the envelope proteins of spinach chloroplasts. In Photosynthesis;from Light to Biosphere. Vol. V. Edited by Mathis, P. pp. 479-482. Kluwer Academic Publisher, Netherlands.