Cytotoxicity of four root canal sealers in permanent 3T3 cells and primary human periodontal ligament fibroblast cultures

Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontics

Volumn 85, Issue 5, 1998, Pages 592-597

  Geurtsen, Werner ^a   Leinenbach, Frank ^a   Krage, Tracey ^a   Leyhausen, Gabriele ^a  

^a HANNOVER MEDICAL SCHOOL   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

AH 26 FILLING MATERIAL; APEXIT; BIOMATERIAL; BISMUTH; CALCIUM HYDROXIDE; COLORING AGENT; DIAGNOSTIC AGENT; EPOXY RESIN; EUGENOL; FORMALDEHYDE; GUTTA PERCHA; METHENAMINE; N2 DENTAL CEMENT; PROPIDIUM IODIDE; ROOT CANAL FILLING MATERIAL; SALICYLIC ACID DERIVATIVE; SEALAPEX; SILVER; TITANIUM; ZINC OXIDE;

ANIMAL; ARTICLE; CELL CULTURE; CELL DIVISION; CELL STRAIN 3T3; COMPARATIVE STUDY; CULTURE MEDIUM; CYTOLOGY; DRUG COMBINATION; DRUG EFFECT; EVALUATION; FIBROBLAST; HUMAN; MATERIALS TESTING; MOUSE; PERIODONTAL LIGAMENT; TIME;

3T3 CELLS; ANIMALS; BIOCOMPATIBLE MATERIALS; BISMUTH; CALCIUM HYDROXIDE; CELL DIVISION; CELLS, CULTURED; COLORING AGENTS; CULTURE MEDIA; DRUG COMBINATIONS; EPOXY RESINS; EUGENOL; EVALUATION STUDIES; FIBROBLASTS; FORMALDEHYDE; GUTTA-PERCHA; HUMANS; MATERIALS TESTING; METHENAMINE; MICE; PERIODONTAL LIGAMENT; PROPIDIUM; ROOT CANAL FILLING MATERIALS; SALICYLATES; SILVER; TIME FACTORS; TITANIUM; ZINC OXIDE;

EID: 0032059886     PISSN: 10792104     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1079-2104(98)90297-9     Document Type: Article

References (31)

1. Murphy WM. The testing of endodontic materials in vitro. Int Endod J 1988;21:170-7.
2. Fanibunda KB. Adverse response to endodontic material containing paraformaldehyde. Br Dent J 1984;157:231-5.
3. Geurtsen W, Leyhausen G. Biological aspects of root canal filling materials: histocompatibility, cytotoxicity, and mutagenicity. Clin Oral Invest 1997;1:5-11.
4. Heil J, Reifferscheid G, Waldmann P, Leyhausen G, Geurtsen W. Genotoxicity of dental materials. Mutat Res 1996;368:181-94.
5. Hong YC, Wang JT, Hong CY, Brown WE, Chow LC. The periapical tissue reactions to a calcium phosphate cement in the teeth of monkeys. J Biomed Mater Res 1991;25:485-98.
6. Lambjerg-Hansen H. Vital pulpectomy and root filling with N2 or endomethasone. Int Endod J 1987;20:194-204.
7. Sonat B, Dalat D, Günhan O. Periapical tissue reaction to root fillings with Sealapex. Int Endod J 1990;23:46-52.
8. Yesiloy C, Koren LZ, Morse DR, Kobayashi C. A comparative tissue toxicity evaluation of established and newer root canal sealers. Oral Surg Oral Med Oral Pathol 1988;65:459-67.
9. Barker BCW, Lockett BC. Reaction of dog tissue to immediate root filling with zinc oxide cement and gutta percha. Aust Dent J 1972;17:1-8.
10. Holland R, De Souza V, Nery MJ, De Mello W, Bernabe PFE, Otoboni Filho JA. Reaction of rat connective tissue to gutta-percha and silver points. A long-term histological study. Aust Dent J 1982;27:224-6.
11. Serene TP, Vessely J, Boackle RJ. Complement activation as a possible in vitro indication of the inflammatory potential of endodontic materials. Oral Surg Oral Med Oral Pathol 1988;65:354-7.
12. Sjögren U, Sundqvist G, Nair PNR. Tissue reaction to gutta-percha particles of various sizes when implanted subcutaneously in guinea pigs. Eur J Oral Sci 1995;103:313-21.
13. Bezerra Silva LA, Leonardo MR, Faccioli LH, Figueiredo F. Inflammatory response to calcium hydroxide based root canal sealers. J Endod 1997;23:86-90.
14. Boiesen J, Brodin P. Neurotoxic effect of two root canal sealers with calcium hydroxide on rat phrenic nerve in vitro. Endod Dent Traumatol 1991;7:242-5.
15. Beltes P, Koulaouzidou E, Kotoula V, Kortsaris AH. In vitro evaluation of the cytotoxicity of calcium hydroxide-based root canal sealers. Endod Dent Traumatol 1995;11:245-9.
16. Al-Nazhan S, Spangberg S. Morphological cell changes due to chemical toxicity of a dental material: an electron microscopical study on human periodontal ligament fibroblasts and L929 cells. J Endod 1990;16:129-34.
17. Matsumoto K, Inoue K, Matsumoto A. The effect of newly developed root canal sealers on rat pulp cells in primary culture. J Endod 1989;15:60-7.
18. Barbosa SV, Burkhard DH, Spangberg LSW. Cytotoxic effects of gutta-percha solvents. J Endod 1994;20:6-9.
19. McNamara JR, Heithersay GS, Wiebkin OW. Cell responses to Hydron by a new in-vitro method. Int Endod J 1992;25:205-12.
20. Nakamura H, Sakakibara F, Matsumoto Y, et al. Study on the cytotoxicity of root canal filling materials. J Endod 1986;12: 156-60.
21. Lehmann F, Leyhausen G, Geurtsen W. Usefulness of primary gingival fibroblast cultures for cytotoxicity tests (abstr 1896). J Dent Res 1996;75:254.
22. Green CE, Dabbs JE, Tyson CA. Metabolism and cytotoxicity of acetaminophen in hepatocytes isolated from resistant and susceptible species. Toxicol Appl Pharmacol 1984;76:139-49.
23. Yamamoto M, Mitsui Y, Ooka H, Yamamoto K. Appearance of the terminal senescent cell population in human diploid fibroblasts analyzed by flow cytometry. Mech Ageing Dev 1990;51: 195-214.
24. ISO: ISO/TC 106 - Dentistry, Committee draft ISO/CD TR 7405, 1994.
25. Ferracane JL, Condon JR. Rate of elution of leachable components from composite. Dent Mater 1990;6:282-7.
26. Munaco FS, Miller WA, Everett MM. A study of long-term toxicity of endodontic materials with use in an in vitro model. J Endod 1978;4:151-7.
27. Pascon EA, Spangberg LSW. In vitro cytotoxicity of root canal filling materials: 1. Gutta-percha. J Endod 1990;16:429-33.
28. 51Cr-release method in cytotoxicity assay of endodontic sealers. J Endod 1997;23:355-7.
29. Spangberg LSW, Barbosa SV, Lavigne GD. AH26 releases formaldehyde. J Endod 1993;19:596-8.
30. Antrim DD: Evaluation of the cytotoxicity of root canal sealing agents on tissue culture cells in vitro: Grossman's sealer, N2 (permanent), Rickert's sealer, and Cavit. J Endod 1976;2:111-6.
31. Lindqvist L, Otteskog P. Eugenol: liberation from dental materials and effect on human diploid fibroblast cells. Scand J Dent Res 1980;88:552-6.