Vimentin immunohistochemistry in human fetal brain: Methods of standard incubation versus thermal intensification achieve different objectives

Pediatric and Developmental Pathology

Volumn 1, Issue 3, 1998, Pages 222-229

  Sarnat, Harvey B ^a,b  

^a UNIVERSITY OF WASHINGTON SCHOOL OF MEDICINE   (United States)

^b SEATTLE CHILDREN S RESEARCH INSTITUTE   (United States)

Author keywords

Fetal CNS; Immunohistochemistry; Microwaves; Vimentin

Indexed keywords

MONOCLONAL ANTIBODY; VIMENTIN;

ARTICLE; ASTROCYTE; BRAIN LEVEL; CHEMISTRY; CHOROID PLEXUS; FETUS; GLIA; HEATING; HUMAN; IMMUNOHISTOCHEMISTRY; METHODOLOGY; MICROWAVE RADIATION; NEWBORN; PRENATAL DEVELOPMENT; SPINAL CORD;

ANTIBODIES, MONOCLONAL; ASTROCYTES; BRAIN CHEMISTRY; CHOROID PLEXUS; FETUS; HEATING; HUMANS; IMMUNOHISTOCHEMISTRY; INFANT, NEWBORN; MICROWAVES; NEUROGLIA; SPINAL CORD; VIMENTIN;

EID: 0032057093     PISSN: 10935266     EISSN: None     Source Type: Journal    
DOI: 10.1007/s100249900030     Document Type: Article

References (19)

1. Schnitzer J, Franke WW, Schachner M. Immunocytochemical demonstration of vimentin in astrocytes and ependymal cells in developing and adult mouse nervous systems. J Cell Biol 1981;90:435-447.
2. Tapscott SJ, Bennett GS, Toyama Y, Kleinhart F, Holtzer H. Intermediate filament proteins in the developing chick spinal cord. Dev Biol 1981;86:40-54.
3. Sasaki A, Hirato J, Nakazato Y, Ishida Y. Immunohistochemical study of the early human fetal brain. Acta Neuropathol 1988;76:128-134.
4. Lukás Z, Dráber P, Bucek J, Dráberova E, Viklický V, Stasková Z. Expression of vimentin and glial fibrillary acidic protein in human developing spinal cord. Histochem J 1989;21:693-701.
5. Gould SJ, Howard S, Papadaki L. The development of ependyma in the human fetal brain: an immunohistological and electron microscopic study. Dev Brain Res 1990;55: 255-267.
6. Honig LS, Herrmann K, Shatz CJ. Developmental changes revealed by immunohistochemical markers in human cerebral cortex. Cerebr Cortex 1996;6:794-806.
7. Sarnat HB. Regional differentiation of the human fetal ependyma: immunocytochemical markers. J Neuropathol Exp Neurol 1992;51:58-75.
8. Sarnat HB. Microscopic criteria to determine gestational age of the fetal and neonatal brain. In: García JH, ed. Neuropathology: The Diagnostic Approach. St. Louis: Mosby, 1997;529-540.
9. Shi SR, Key ME, Kalra KL. Antigen retrieval in formalin-fixed, paraffin-embedded tissues: an enhancement method for immunohistochemical staining based on microwave oven heating of tissue sections. J Histochem Cytochem 1991;39:741-748.
10. Kok LP, Boon ME. Microwave Cookbook for Microscopists, 3rd ed. Leyden: Coulomb Press, 1992.
11. Marani E, Horobin RW. Overview of microwave applications in the neurosciences. J Neurosci Methods 1994;55: 111-117.
12. Ainley CD, Ironside JW. Microwave technology in diagnostic neuropathology. J Neurosci Methods 1994;55:183-190.
13. Login GR, Dvorak AM. Application of microwave fixation techniques in pathology to neuroscience studies: a review. J Neurosci Methods 1994;55:1733-1782.
14. Suurmeyer AJH, Boon ME. Optimizing keratin and vimentin retrieval in formalin-fixed paraffin-embedded tissue with the use of heat and metal salts. Appl Immunohistochem 1993;1:143-148.
15. Evers P, Uylings HBM. Microwave-stimulated antigen retrieval is pH and temperature dependent. J Histochem Cytochem 1994;42:1555-1563.
16. Feirabend HKP, Ploeger S, Kok P, Chouroer H. Does microwave irradiation have other than thermal effects on histological staining of the mammalian CNS? Microwave Newsletter 7. Eur J Morphol 1992;30:312-327.
17. Roessmann U, Velasco ME, Sindely SD, Gambetti P. Glial fibrillary acidic protein (GFAP) in ependymal cells during development. An immunocytochemical study. Brain Res 1980;200:13-21.
18. Eng LF. Glial fibrillary acidic protein (GFAP): the major protein of glial intermediate filaments in differentiating astrocytes. J Neuroimmunol 1985;8:203-214.
19. Choi BH. Glial fibrillary acidic protein in radial glial of early human fetal cerebrum: a light and electron microscopic immunoperoxidase study. J Neuropathol Exp Neurol 1986;45:408-418.