Chromosomal alterations, biological features and in vitro chemosensitivity of SCLC-R1, a new cell line from human metastatic small cell lung carcinoma

European Journal of Cancer

Volumn 34, Issue 5, 1998, Pages 724-730

  Gasperi Campani, A ^a   Roncuzzi, L ^a   Zoli, W ^b   Lenzi, L ^c   Gruppioni, R ^d   Sensi, A ^e   Zini, N ^a   Farabegoli, F ^a   Amadori, D ^b  

^a UNIVERSITY OF BOLOGNA   (Italy)

^b Natl Inst for the Res on Cancer   (Italy)

^c MORGAGNI PIERANTONI HOSPITAL   (Italy)

^d UNIVERSITY OF FERRARA   (Italy)

^e CNR   (Italy)

Author keywords

Cell line establishment; Chemosensitivity; DNA index; Gene amplification; Gene mutation; Karyotype; Patient's clinical history; Small cell lung carcinoma; Tumour marker; Ultrastructural features

Indexed keywords

ARTICLE; CANCER CELL CULTURE; CHEMICAL CARCINOGENESIS; CHEMOSENSITIVITY; CHROMOSOME ABERRATION; HUMAN; HUMAN CELL; LUNG METASTASIS; LUNG SMALL CELL CANCER; PRIORITY JOURNAL;

AGED; ANTINEOPLASTIC AGENTS; CARCINOMA, SMALL CELL; CHROMOSOME ABERRATIONS; DIPLOIDY; DNA, NEOPLASM; GENE DELETION; HUMANS; LUNG NEOPLASMS; MALE; PROTO-ONCOGENE PROTEINS; TRANSLOCATION, GENETIC; TUMOR CELLS, CULTURED; TUMOR MARKERS, BIOLOGICAL;

EID: 0032055635     PISSN: 09598049     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0959-8049(97)10148-4     Document Type: Article

References (28)

1. Boring CG, Squires TS, Tong T, Montgomery S. Cancer statistics. Ca-Cancer J Clin 1994, 44, 7-26.
2. Makela TP, Mattson K, Alitalo K. Tumour markers and oncogenes in lung cancer. Eur J Cancer 1991, 27A, 1323-1327.
3. World Health Organization. International Histological Classification of Tumors, second edition. Geneva, WHO, 1982.
4. Gazdar AF. The molecular and cellular basis of human lung cancer. Anticancer Res 1994, 13, 261-268.
5. Rabbitts PH. Genetic changes in the development of lung cancer. BrMed Bull 1994, 50, 688-697.
6. Goguel AF, Fouquet F, Duverger A, et al. Evolution of chromosomal alterations and biologic features in two small cell lung carcinoma cell lines established from one patient during the course of the disease. Cancer Genet Cytogenet 1995, 80, 47-54.
7. Campling BG, Haworth AC, Baker HM, et al. Establishment and characterisation of a panel of human lung cancer cell lines. Cancer 1992, 69, 2064-2074.
8. Negrini M, Castagnoli A, Pavan JW, et al. Suppression of tumorigenicity and anchorage-independent growth of BK virustransformed mouse cells by human chromosome 11. Cancer Res 1992,52, 1297-1303.
9. Dutrillaux B, Lejeune J. New techniques in the study of human chromosomes: methods and applications. Adv Hum Genet 1975, 5, 119-156.
10. Seabright M. The use of proteolytic enzymes for the mapping of structural rearrangements in the chromosomes of man. Chromosoma 1972,36, 204-210.
11. Baens M, Aerssens J, Van Zand K, Van Der Berghe H, Marynen P. Isolation and regional assignment of human chromosome 12p cDNAs. Genomics 1995, 29, 44-52.
12. Farabegoli F, Ceccarelli G, Santini D, et al. Chromosome 1 aneusomy with Ip36 under-representation is related to histologie grade, DNA aneuploidy, high c-erb B-2 and loss of bcl-2 expression in ductal breast carcinoma. IntJ Cancer (pred Oncol) 1996, 69, 381-385.
13. Ausubel FM, Brent R, Kingston RE, et al. In Current Protocols in Molecular Biology. New York, Wiley and Sons, 1989.
14. Southern EM. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol 1975, 98, 503-517.
15. Dalla Favera R, Martinotti S, Gallo RC, Erikson J, Grace CM. Translocation and rearrangements of the c-myc oncogene locus in human undifferentiated B-cell lymphomas. Science 1983, 219, 963-967.
16. King CR, Kraus MH, Aaronson SA. Amplification of a novel verbB-related gene in a human mammary carcinoma. Science 1985, 229, 974-976.
17. Casey G, Smith R, McGillivray D, Peters G, Dickson G. Characterisation and chromosome assignment of the human homolog of int-2, a potential proto-oncogene. Mol Cell Biol 1986, 6, 502-510.
18. Watson R, Oskarsson M, Vande Woude GF. Human DNA sequence homologous to the transforming gene (mos) of Moloney sarcoma virus. Proc NatlAcadSd USA 1982, 79, 4078-4082.
19. Ranzani GN, Pellegata NS, Previderè C, et al. Heterogeneous protooncogene amplification correlates with tumor progression and presence of métastases in gastric cancer patients. Cancer Res 1990, 50, 7811-7814.
20. Taira M, Yoshida T, Miyagawa K, Sakamoto H, Terada M, Sugimura T. cDNA sequence of human transforming gene hst and identification of the coding sequence required for transforming activity. Proc NatlAcadSd USA 1987, 84, 2980-2984.
21. Pulciani S, Santos E, Lauver AV, Long LK, Barbacid M. Transforming genes in human tumors. J Cell Biochem 1982, 20, 51-61.
22. Nau MM, Brooks BJ, Battey J, et al. L-myc, a new myc-related gene amplified and expressed in human small cell lung cancer. Nature 1985,318, 69-73.
23. Schwab M, Alitalo K, Klempnauer KH, et al. Amplified DNA with limited homology to myc cellular oncogene is shared by human neuroblastoma cell lines and a neuroblastoma tumour. Nature 1983, 305, 245-248.
24. Orita M, Suzuki Y, Sekiya T, Hayashi K. Rapid and sensitive detection of point mutations and DNA polymorphisms using the polymerase chain reaction. Genomics 1989, 5, 874-879.
25. Zoli W, Roncuzzi L, Flamigni A, et al. A new cell line from human infiltrating ductal carcinoma of the breast: establishment and characterization. J Cancer Res Clin Oncol 1996, 122, 237242.
26. Tanigawa N, Kern DH, Hikasa Y, Morton DL. Rapid assay for evaluating the chemosensitivity of human tumors in soft agar culture. Cancer Res 1982, 42, 2159-2164.
27. Spinazzi A, Soresi E, Boffi R, et al. Evaluation of neuron-specific enolase, tissue polypeptide antigen, and carcinoembryonic antigen as markers for staging and monitoring response to therapy of lung cancer. Cancer Detect Prev 1994, 18, 209-220.
28. Yanagisawa K, Kondo M, Osada H, et al. Molecular analysis of FHIT gene at 3pl4.2 in lung cancer cell lines. Cancer Res 1996, 56, 5579-5582.